scholarly journals Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

2014 ◽  
pp. 775 ◽  
Author(s):  
Sara Svensson ◽  
Magnus Forsberg ◽  
Mats Hulander ◽  
Forugh Vazirisani ◽  
Peter Thomsen ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Monocyte Activation ◽  
Gold Surfaces ◽  
Nanostructured Gold

scholarly journals Staphylococcus epidermidis in Orthopedic Device Infections: The Role of Bacterial Internalization in Human Osteoblasts and Biofilm Formation

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67240 ◽  
Author(s):  
Florent Valour ◽  
Sophie Trouillet-Assant ◽  
Jean-Philippe Rasigade ◽  
Sébastien Lustig ◽  
Emmanuel Chanard ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Human Osteoblasts ◽  
Orthopedic Device

Role of proton-motive force in adhesion and biofilm formation by staphylococcus epidermidis

Microbiology ◽  
2016 ◽  
Vol 85 (4) ◽  
pp. 506-508
Author(s):  
D. V. Eroshenko ◽  
T. V. Polyudova ◽  
V. P. Korobov
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Proton Motive Force ◽  
Motive Force

scholarly journals The Teicoplanin-Associated Locus Regulator (TcaR) and the Intercellular Adhesin Locus Regulator (IcaR) Are Transcriptional Inhibitors of the ica Locus in Staphylococcus aureus

2004 ◽  
Vol 186 (8) ◽  
pp. 2449-2456 ◽  
Author(s):  
Kimberly K. Jefferson ◽  
Danielle B. Pier ◽  
Donald A. Goldmann ◽  
Gerald B. Pier
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Negative Regulator ◽  
Regulatory Proteins ◽  
Polysaccharide Intercellular Adhesin ◽  
Topoisomerase Iv ◽  
Exogenous Factors ◽  
Dna Affinity Chromatography

ABSTRACT Infections involving Staphylococcus aureus are often more severe and difficult to treat when the organism assumes a biofilm mode of growth. The polysaccharide poly-N-acetylglucosamine (PNAG), also known as polysaccharide intercellular adhesin, is synthesized by the products of the intercellular adhesin (ica) locus and plays a key role in biofilm formation. Numerous conditions and exogenous factors influence ica transcription and PNAG synthesis, but the regulatory factors and pathways through which these environmental stimuli act have been only partially characterized. We developed a DNA affinity chromatography system to purify potential regulatory proteins that bind to the ica promoter region. Using this technique, we isolated four proteins, including the staphylococcal gene regulator SarA, a MarR family transcriptional regulator of the teicoplanin-associated locus TcaR, DNA-binding protein II, and topoisomerase IV, that bound to the ica promoter. Site-directed deletion mutagenesis of tcaR indicated that TcaR was a negative regulator of ica transcription, but deletion of tcaR alone did not induce any changes in PNAG production or in adherence to polystyrene. We also investigated the role of IcaR, encoded within the ica locus but divergently transcribed from the biosynthetic genes. As has been shown previously in Staphylococcus epidermidis, we found that IcaR was also a negative regulator of ica transcription in S. aureus. We also demonstrate that mutation of icaR augmented PNAG production and adherence to polystyrene. Transcription of the ica locus, PNAG production, and adherence to polystyrene were further increased in a tcaR icaR double mutant. In summary, TcaR appeared to be a weak negative regulator of transcription of the ica locus, whereas IcaR was a strong negative regulator, and in their absence PNAG production and biofilm formation were enhanced.

scholarly journals The Glycosyltransferases sdgA and sdgB Expression in Staphylococcus Epidermidis Depends On The Conditions of Biofilm Formation.

2021 ◽  
Author(s):  
Itzia S. Gómez-Alonso ◽  
Ilse D. Estrada-Alemán ◽  
Sergio Martínez-García ◽  
Humberto Peralta ◽  
Erika T. Quintana ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Cathepsin G ◽  
Dynamic Conditions ◽  
Static Conditions ◽  
Sessile Cells ◽  
In Cells

Abstract The Staphylococcus aureus’ SdrG protein is glycosylated by SdgA and SdgB for their protection against its degradation by the neutrophil’s cathepsin G. So far, there is not information about the role of Staphylococcus epidermidis’ SdgA nor SdgB in the production of biofilm, therefore the main of this work was to determine the distribution and expression of sdrG, sdgA and sdgB genes in S. epidermidis in conditions of biofilm. The frequency of the genes sdrG, sdgA and sdgB were evaluated by PCR in a collection of 75 isolates. The isolates were grown in dynamic conditions (in agitation) or static conditions (biofilm productor: planktonic or sessile cells). The expression of sdrG, sdgA and sdgB were determined by RT-qPCR in cells grown under dynamic conditions (CGDC), as well as planktonic and sessile cells, and in cells adhered to a catheter (in vivo). The genes sdrG and sdgB were detected in 100% of isolates, meanwhile the gene sdgA was detected in 71% of the samples (p<0.001). The CGDC did not expressed the sdrG, sdgA and sdgB mRNAs. The planktonic and sessile cells expressed sdrG and sdgB, and the same was seen in cells adhered to the catheter. In particular, one isolate, able to induce biofilm under cathepsin G treatment, expressed sdrG and sdgB in planktonic, sessile and in cells adhered to the catheter. This suggests that the state of cells adherence is an important factor for the transcription of sdgA, sdgB and sdrG.

scholarly journals The Terminal A Domain of the Fibrillar Accumulation-Associated Protein (Aap) of Staphylococcus epidermidis Mediates Adhesion to Human Corneocytes

2009 ◽  
Vol 191 (22) ◽  
pp. 7007-7016 ◽  
Author(s):  
Robin L. Macintosh ◽  
Jane L. Brittan ◽  
Ritwika Bhattacharya ◽  
Howard F. Jenkinson ◽  
Jeremy Derrick ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Domain Architecture ◽  
Opportunistic Pathogen ◽  
Additional Function ◽  
Artificial Joints ◽  
A Cell ◽  
Skin Colonization ◽  
Accumulation Associated Protein

ABSTRACT The opportunistic pathogen Staphylococcus epidermidis colonizes indwelling medical devices by biofilm formation but is primarily a skin resident. In many S. epidermidis strains biofilm formation is mediated by a cell wall-anchored protein, the accumulation-associated protein (Aap). Here, we investigate the role of Aap in skin adhesion. Aap is an LPXTG protein with a domain architecture including a terminal A domain and a B-repeat region. S. epidermidis NCTC 11047 expresses Aap as localized, lateral tufts of fibrils on one subpopulation of cells (Fib+), whereas a second subpopulation does not express these fibrils of Aap (Fib−). Flow cytometry showed that 72% of NCTC 11047 cells expressed Aap and that 28% of cells did not. Aap is involved in the adhesion of Fib+ cells to squamous epithelial cells from the hand (corneocytes), as the recombinant A-domain protein partially blocked binding to corneocytes. To confirm the role of the Aap A domain in corneocyte attachment, Aap was expressed on the surface of Lactococcus lactis MG1363 as sparsely distributed, peritrichous fibrils. The expression of Aap increased corneocyte adhesion 20-fold compared to L. lactis carrying Aap without an A domain. S. epidermidis isolates from catheters, artificial joints, skin, and the nose also used the A domain of Aap to adhere to corneocytes, emphasizing the role of Aap in skin adhesion. In addition, L. lactis expressing Aap with different numbers of B repeats revealed a positive correlation between the number of B repeats and adhesion to corneocytes, suggesting an additional function for the B region in enhancing A-domain-dependent attachment to skin. Therefore, in addition to its established role in biofilm formation, Aap can also promote adhesion to corneocytes and is likely to be an important adhesin in S. epidermidis skin colonization.

Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis

2007 ◽  
Vol 9 (11) ◽  
pp. 1376-1383 ◽  
Author(s):  
Chongzhen Wang ◽  
Min Li ◽  
Dandan Dong ◽  
Jianping Wang ◽  
Jun Ren ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation

scholarly journals Essential Functional Role of the Polysaccharide Intercellular Adhesin of Staphylococcus epidermidis in Hemagglutination

1999 ◽  
Vol 67 (2) ◽  
pp. 1004-1008 ◽  
Author(s):  
Dietrich Mack ◽  
Joachim Riedewald ◽  
Holger Rohde ◽  
Tim Magnus ◽  
Hubert H. Feucht ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Functional Role ◽  
Common Property ◽  
Polysaccharide Intercellular Adhesin ◽  
Functional Component ◽  
Negative Phenotype

ABSTRACT Hemagglutination of erythrocytes is a common property ofStaphylococcus epidermidis strains, which is related to adherence and biofilm formation and may be essential for the pathogenesis of biomaterial-associated infections caused byS. epidermidis. In three independent biofilm-producing, hemagglutination-positive S. epidermidis isolates, interruption of the icaADBC operon essential for polysaccharide intercellular adhesin (PIA) synthesis by Tn917 insertions led to a hemagglutination-negative phenotype. An immunoglobulin G fraction of antiserum to PIA greatly reduced hemagglutination. Purified PIA led to a 64-fold decrease of hemagglutination titers of these strains; however, it did not mediate hemagglutination by itself. These observations define PIA as the hemagglutinin of S. epidermidis or at least as its major functional component.

scholarly journals The Possible Role of Staphylococcus epidermidis LPxTG Surface Protein SesC in Biofilm Formation

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146704 ◽  
Author(s):  
Laleh Khodaparast ◽  
Ladan Khodaparast ◽  
Mohammad Shahrooei ◽  
Benoit Stijlemans ◽  
Rita Merckx ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Surface Protein

scholarly journals Identification of the sigB Operon inStaphylococcus epidermidis: Construction and Characterization of a sigB Deletion Mutant

2001 ◽  
Vol 69 (12) ◽  
pp. 7933-7936 ◽  
Author(s):  
Stefanie Kies ◽  
Michael Otto ◽  
Cuong Vuong ◽  
Friedrich Götz
Keyword(s):  
Staphylococcus Epidermidis ◽  
Sigma Factor ◽  
Biofilm Formation ◽  
Deletion Mutant ◽  
Alternative Sigma Factor ◽  
Global Regulator ◽  
Profound Influence ◽  
Cytoplasmic Proteins

ABSTRACT The role of the alternative sigma factor ςB inStaphylococcus epidermidis was investigated by the construction, complementation, and characterization of asigB deletion mutant. Electrophoretic analyses confirmed a profound influence of ςB on the expression of exoproteins and cytoplasmic proteins. Detailed investigation revealed reduced lipase and enhanced protease activity in the ςB mutant. Furthermore, no significant influence of ςB on heterologous biofilm formation or on the activity of the global regulator agr was detected.

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