scholarly journals The exposure of cancer cells to hyperthermia, iron oxide nanoparticles, and mitomycin C influences membrane multidrug resistance protein expression levels

2013 ◽  
pp. 351 ◽  
Author(s):  
Ingrid Hilger ◽  
Franke ◽  
Kettering ◽  
Lange ◽  
Kaiser
Keyword(s):  
Multidrug Resistance ◽  
Iron Oxide ◽  
Protein Expression ◽  
Cancer Cells ◽  
Mitomycin C ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Multidrug Resistance Protein ◽  
Expression Levels ◽  
Resistance Protein

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

scholarly journals Doxorubicin loaded iron oxide nanoparticles overcome multidrug resistance in cancer in vitro

2011 ◽  
Vol 152 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Forrest M. Kievit ◽  
Freddy Y. Wang ◽  
Chen Fang ◽  
Hyejung Mok ◽  
Kui Wang ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Effects of quercetin-conjugated with superparamagnetic iron oxide nanoparticles on learning and memory improvement through targeting microRNAs/NF-κB pathway

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shiva Ebrahimpour ◽  
Abolghasem Esmaeili ◽  
Fariba Dehghanian ◽  
Siamak Beheshti
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Diabetic Rats ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Expression Levels ◽  
Memory Improvement ◽  
Tnf Α ◽  
Ameliorative Effect

Abstract Quercetin-conjugated superparamagnetic iron oxide nanoparticles (QCSPIONs) have an ameliorative effect on diabetes-induced memory impairment. The current study aimed to compare the effect of quercetin (QC) and QCSPIONs on inflammation-related microRNAs and NF-κB signaling pathways in the hippocampus of diabetic rats. The expression levels of miR-146a, miR-9, NF-κB, and NF-κB-related downstream genes, including TNF-α, BACE1, AβPP, Bax, and Bcl-2 were measured using quantitative real-time PCR. To determine the NF-κB activity, immunohistochemical expression of NF-κB/p65 phosphorylation was employed. Computer simulated docking analysis also performed to find the QC target proteins involved in the NF-κB pathway. Results indicate that diabetes significantly upregulated the expression levels of miR-146a, miR-9, TNF-α, NF-κB, and subsequently AβPP, BACE1, and Bax. Expression analysis shows that QCSPIONs are more effective than pure QC in reducing the expression of miR-9. Interestingly, QCSPIONs reduce the pathological activity of NF-κB and subsequently normalize BACE1, AβPP, and the ratio of Bax/Bcl-2 expression better than pure QC. Comparative docking analyses also show the stronger binding affinity of QC to IKK and BACE1 proteins compared to specific inhibitors of each protein. In conclusion, our study suggests the potent efficacy of QCSPIONs as a promising drug delivery system in memory improvement through targeting the NF-κB pathway.

scholarly journals Size-Dependent Thermotherapy of Iron Oxide Nanoparticles on Human Breast Adenocarcinoma Cancer Cells

2013 ◽  
Vol 19 (S2) ◽  
pp. 218-219
Author(s):  
T. Mustafa ◽  
Y. Xu ◽  
F. Watanabe ◽  
Y. Zhang ◽  
M. Asar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Adenocarcinoma ◽  
Oxide Nanoparticles ◽  
Human Breast ◽  
Size Dependent ◽  
Human Breast Adenocarcinoma

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

scholarly journals Dendrimer-Functionalized Iron Oxide Nanoparticles for Specific Targeting and Imaging of Cancer Cells

2007 ◽  
Vol 17 (16) ◽  
pp. 3043-3050 ◽  
Author(s):  
S. H. Wang ◽  
X. Shi ◽  
M. Van Antwerp ◽  
Z. Cao ◽  
S. D. Swanson ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Specific Targeting

Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells

2010 ◽  
Vol 322 (15) ◽  
pp. 2244-2250 ◽  
Author(s):  
Mar Creixell ◽  
Adriana P. Herrera ◽  
Vanessa Ayala ◽  
Magda Latorre-Esteves ◽  
Marianela Pérez-Torres ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Iron Oxide ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Colon Cancer Cells ◽  
Epidermal Growth

scholarly journals Quercetin-conjugated superparamagnetic iron oxide nanoparticles (QCSPIONs) increases Nrf2 expression via miR-27a mediation to prevent memory dysfunction in diabetic rats

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shiva Ebrahimpour ◽  
Seyedeh Bahar Shahidi ◽  
Mahnoosh Abbasi ◽  
Zahra Tavakoli ◽  
Abolghasem Esmaeili
Keyword(s):  
Oxidative Stress ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Diabetic Rats ◽  
Expression Level ◽  
Oxide Nanoparticles ◽  
Antioxidant Genes ◽  
Expression Levels ◽  
Nrf2 Expression

Abstract Oxidative stress is one of the earliest defects involved in the development of diabetes-induced cognitive impairment. Nrf2 is the master regulator of the cellular antioxidant system can be regulated by some microRNAs. The study aimed to evaluate the effects of quercetin (QC) and quercetin-conjugated superparamagnetic iron oxide nanoparticles (QCSPIONs) on Nrf2-controlled antioxidant genes through the redox-sensitive miR-27a. Expression levels of miR-27a, Nrf2, SOD1, GPX1, and CAT were measured by quantitative real-time PCR. Moreover, the oxidative stress parameters including total antioxidant capacity (TAC) and histological alterations were investigated. The expression level of miR-27a was significantly up-regulated in diabetic rats. While expression levels of Nrf2, SOD1, GPX1, and CAT were significantly down-regulated under diabetic condition. Interestingly, QCSPIONs decreased expression level of miR-27a and subsequently enhanced the expression levels of Nrf2, SOD1, and CAT to the control level. No significant difference was observed in the expression level of GPX1. Besides, QC in pure and especially conjugated form was able to normalize TAC and regenerate pathological lesions in STZ-diabetic rats. Our result demonstrates that QCSPIONs as an effective combined therapy can decrease miR-27a expression, which in turn increases the Nrf2 expression and responsive antioxidant genes, resulting in improvement of memory dysfunction in diabetic rats.

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