scholarly journals Low molecular weight chitosan conjugated with folate for siRNA delivery in vitro: optimization studies

2012 ◽  
pp. 5833 ◽  
Author(s):  
Qin Shi ◽  
Julio Fernandes ◽  
Francoise Winnik ◽  
Mohamed Benderdour ◽  
Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Sirna Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan

scholarly journals Low-Molecular-Weight Polyethyleneimine Grafted Polythiophene for Efficient siRNA Delivery

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Pan He ◽  
Kyoji Hagiwara ◽  
Hui Chong ◽  
Hsiao-hua Yu ◽  
Yoshihiro Ito
Keyword(s):  
Molecular Weight ◽  
Sirna Delivery ◽  
Fluorescent Microscopy ◽  
Molar Ratio ◽  
Low Molecular Weight ◽  
Label Free ◽  
Endosomal Membrane ◽  
Cationic Copolymers ◽  
Interfering Rna

Owing to its hydrophilicity, negative charge, small size, and labile degradation by endogenous nucleases, small interfering RNA (siRNA) delivery must be achieved by a carrier system. In this study, cationic copolymers composed of low-molecular-weight polyethylenimine and polythiophenes were synthesized and evaluated as novel self-tracking siRNA delivery vectors. The concept underlying the design of these copolymers is that hydrophobicity and rigidity of polythiophenes should enhance the transport of siRNA across the cell membrane and endosomal membrane. A gel retardation assay showed that the nanosized complexes formed between the copolymers and siRNA were stable even at a molar ratio of 1 : 2. The high cellular uptake (>80%) and localization of the copolymer vectors inside the cells were easily analyzed by tracking the fluorescence of polythiophene using fluorescent microscopy and cytometry. Anin vitroluciferase knockdown (KD) assay in A549-luc cells demonstrated that the siRNA complexes with more hydrophobic copolymers achieved a higher KD efficiency of 52.8% without notable cytotoxicity, indicating protein-specific KD activity rather than solely the cytotoxicity of the materials. Our polythiophene copolymers should serve as novel, efficient, low cell toxicity, and label-free siRNA delivery systems.

scholarly journals Nematicidal activity of a biopolymer chitosan at different molecular weights against root-knot nematode, Meloidogyne incognita

2012 ◽  
Vol 48 (No. 4) ◽  
pp. 170-178 ◽  
Author(s):  
M.S. Khalil ◽  
M.E.I. Badawy
Keyword(s):  
Molecular Weight ◽  
Meloidogyne Incognita ◽  
Nematicidal Activity ◽  
Egg Mass ◽  
Low Molecular Weight ◽  
Root Knot Nematode ◽  
Molecular Weights ◽  
Tomato Seedlings ◽  
Low Molecular Weight Chitosan

The nematicidal activity of four molecular weights (2.27 &times; 10<sup>5</sup>, 3.60 &times; 10<sup>5</sup>, 5.97 &times; 10<sup>5</sup>, and 9.47 &times; 10<sup>5</sup> g/mol) of a biopolymer chitosan was assayed against the root-knot nematode, Meloidogyne incognita, in vitro and in pot experiments. In laboratory assays, the nematode mortality was significantly influenced by exposure times and chitosan molecular weight. Low molecular weight chitosan (2.27 &times; 10<sup>5</sup> g/mol) was the most effective in killing the nematode with EC<sub>50</sub> of 283.47 and 124.90 mg/l after 24 and 48 h of treatment, respectively. In a greenhouse bioassay, all the compounds mixed in soil at one- and five-fold concentrations of the LC<sub>50</sub> value significantly reduced population, egg mass, and root galling of tomato seedlings compared with the untreated control. In general, the nematicidal activity of these compounds was increased dramatically with a decrease in the molecular weight. The results suggest that the chitosan at low molecular weight may serve as a natural nematicide

Low molecular weight chitosan–protamine conjugate for siRNA delivery with enhanced stability and transfection efficiency

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110951-110963 ◽  
Author(s):  
Sushilkumar Patil ◽  
Priyanka Bhatt ◽  
Rohan Lalani ◽  
Jitendra Amrutiya ◽  
Imran Vhora ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Sirna Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan ◽  
Low Cytotoxicity ◽  
Enhanced Stability

Chitosan is among the few polymers with high biocompatibility and low cytotoxicity.

The effect of low molecular weight Chitosan on bone resorption in vitro and in vivo

Phytomedicine ◽  
1999 ◽  
Vol 6 (5) ◽  
pp. 305-310 ◽  
Author(s):  
Huiying Li ◽  
Tatsuro Miyahara ◽  
Yasuhiro Tezuka ◽  
Makoto Watanabe ◽  
Nobuo Nemoto ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Resorption ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan

A novel tyrosine-modified low molecular weight polyethylenimine (P10Y) for efficient siRNA delivery in vitro and in vivo

2016 ◽  
Vol 230 ◽  
pp. 13-25 ◽  
Author(s):  
Alexander Ewe ◽  
Susanne Przybylski ◽  
Jana Burkhardt ◽  
Andreas Janke ◽  
Dietmar Appelhans ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Sirna Delivery ◽  
Low Molecular Weight
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