scholarly journals Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers

2012 ◽  
pp. 4861 ◽  
Author(s):  
Ming-Kung Yeh ◽  
Chieh-shen Hu ◽  
Chiao-Hsi Chiang ◽  
Po-da Hong
Keyword(s):  
Chondroitin Sulfate ◽  
Chitosan Nanoparticles ◽  
Cell Uptake ◽  
Cell Monolayers

Novel protein-loaded chondroitin sulfate-N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan nanoparticles with reverse zeta potential: preparation, characterization, and ex vivo assessment

2015 ◽  
Vol 3 (44) ◽  
pp. 8729-8737 ◽  
Author(s):  
Tsung-Neng Tsai ◽  
Hui-Ju Yen ◽  
Cheng-cheung Chen ◽  
Ying-chuan Chen ◽  
Yen-an Young ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Chondroitin Sulfate ◽  
Ex Vivo ◽  
Chitosan Nanoparticles ◽  
Charged Nanoparticles ◽  
Potential Preparation ◽  
Novel Protein

Positively and negatively surface charged nanoparticles were prepared by a facile PEC method composed of chondroitin sulfate and N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan.

scholarly journals Fibroblasts induce heparin synthesis in chondroitin sulfate E containing human bone marrow-derived mast cells

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1188-1195
Author(s):  
L Gilead ◽  
O Bibi ◽  
E Razin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Chondroitin Sulfate ◽  
Alcian Blue ◽  
Proliferation Rate ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Thymidine Uptake ◽  
Cell Monolayers ◽  
Chondroitin Sulfate E

Human bone marrow-derived mast cells (hBMMCs), differentiated in vitro in suspension culture and under the influence of human peripheral blood mononuclear cells conditioned medium (hCM), were tested for their response to recombinant human interleukin-3 (rhIL-3) and for their behavior in different microenvironments. The hBMMCs were incubated in the presence of rhIL-3 and the changes in their proliferation rate were determined. Recombinant hIL-3 induced a more than sixfold increase in 3H-thymidine uptake into the hBMMC DNA in a dose-dependent manner. Human CM used as a control for proliferation response induced a more than eightfold maximal proliferation rate increase. Rabbit anti-rhIL-3 completely inhibited hBMMC 3H-thymidine uptake induced by rhIL-3 and decreased the hCM-induced proliferation by approximately 50%. These hBMMCs were cocultured with four different mytomicin C-treated cell monolayers and assayed for phenotypic changes. After only 2 days in coculture with either embryonic mouse skin-derived fibroblasts (MESFs) or human skin-derived fibroblasts (HSFs), a marked increase in granule number and density was noted on staining with toluidine blue. Mast cells that initially stained alcian blue+/safranin- at day 0 of coculture became alcian blue+/safranin+ during the coculture period. Human BMMC proteoglycan synthesis shifted from approximately 85% chondroitin sulfate E to approximately 60% heparin within 14 to 19 days of coculture with the MESF monolayer and to approximately 50% heparin within 19 days of coculture with the HSF monolayer. None of the above- mentioned changes were noted in cocultures of hBMMCs with 3T3 cell line fibroblast monolayers or in cocultures with bovine vascular endothelium (BVE) cell monolayers. These results demonstrate microenvironmental effects exerted by the MESF and HSF monolayers on IL-3-dependent hBMMCs similar to those reported in the conversion of murine mast cell phenotype.

scholarly journals Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1862 ◽  
Author(s):  
Julieta C. Imperiale ◽  
Inbar Schlachet ◽  
Marianela Lewicki ◽  
Alejandro Sosnik ◽  
Mirna M. Biglione
Keyword(s):  
Interferon Alpha ◽  
Viral Infections ◽  
Chitosan Nanoparticles ◽  
Area Under The Curve ◽  
Hydrodynamic Diameter ◽  
Cell Compatibility ◽  
Cell Monolayers ◽  
Pegylated Nanoparticles ◽  
Oral Pharmacokinetics ◽  
Poly Ethylene

Interferon alpha (IFNα) is a protein drug used to treat viral infections and cancer diseases. Due to its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability, which is associated with severe side effects. We hypothesized that the nanoencapsulation of IFNα within nanoparticles of the mucoadhesive polysaccharide chitosan would improve the oral bioavailability of this drug. In this work, we produced IFNα-loaded chitosan nanoparticles by the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index and concentration were characterized by dynamic light scattering and nanoparticle tracking analysis. After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability of unmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured in monoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated that the nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the study of the oral pharmacokinetics of nanoencapsulated IFNα in BalbC mice revealed two maxima and area-under-the-curve of 56.9 pg*h/mL.

scholarly journals Effect of surface hydrophobicity of therapeutic protein loaded in polyelectrolyte nanoparticles on transepithelial permeability

2018 ◽  
Vol 68 (3) ◽  
pp. 275-293 ◽  
Author(s):  
Ana Miklavžin ◽  
Mateja Cegnar ◽  
Janez Kerč ◽  
Julijana Kristl
Keyword(s):  
Oral Delivery ◽  
Chitosan Nanoparticles ◽  
Hydrophobic Surface ◽  
Surface Hydrophobicity ◽  
Recombinant Erythropoietin ◽  
Protein Drugs ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Transepithelial Permeability ◽  
Polyelectrolyte Complexation

Abstract Oral delivery of protein drugs is greatly limited by low hydrophobicity, an important determinant for intestinal epithelial permeation and bioavailability. Herein, surface properties of recombinant erythropoietin were investigated using the fluorescent dye bis-ANS to monitor relative hydrophobicity for correlation with permeabilities with Caco-2 cells. At various pHs, bis-ANS fluorescence intensity indicated different surface hydrophobicities of erythropoietin molecules. Erythropoietin incorporated in chitosan or chitosan-trimethylchitosan (CS-TMC) nanoparticles prepared by polyelectrolyte complexation and ionotropic gelation with tripolyphosphate also showed different surface hydrophobicities. Chitosan nanoparticles with erythropoietin provided the most hydrophobic surface, followed by free erythropoietin (in water) and that loaded into CS-TMC nanoparticles. Chitosan nanoparticles were more effective than CS-TMC nanoparticles for permeation of erythropoietin across Caco-2 cell monolayers; the lowest permeability was shown by erythropoietin itself. Thus, hydrophilic protein molecules complexed with polyelectrolytes can provide more hydrophobic surfaces that enhance transepithelial permeability. This bis-ANS method also provides valuable information for the design of polyelectrolyte nanoparticules for oral delivery of protein drugs.

Novel protein-loaded chondroitin sulfate–chitosan nanoparticles: Preparation and characterization

2011 ◽  
Vol 7 (10) ◽  
pp. 3804-3812 ◽  
Author(s):  
Ming-kung Yeh ◽  
Kuang-ming Cheng ◽  
Chieh-shen Hu ◽  
Yu-chuan Huang ◽  
Jenn-jong Young
Keyword(s):  
Chondroitin Sulfate ◽  
Chitosan Nanoparticles ◽  
Novel Protein

scholarly journals Effects of Saponins from the Root Bark of Aralia elata on the Transport of Chondroitin Sulfate in Caco-2 Cell Monolayers and Rats

2005 ◽  
Vol 28 (6) ◽  
pp. 1043-1048 ◽  
Author(s):  
Joon-Soo Sim ◽  
Hai Lin Zhao ◽  
Da Wei Li ◽  
So Yean Cho ◽  
Choon Sik Jeong ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Root Bark ◽  
Cell Monolayers ◽  
Aralia Elata

scholarly journals Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 298 ◽  
Author(s):  
Jing M. Yong ◽  
Julia Mantaj ◽  
Yiyi Cheng ◽  
Driton Vllasaliu
Keyword(s):  
Intestinal Epithelium ◽  
Epithelial Transport ◽  
Cell Uptake ◽  
Intestinal Epithelial ◽  
Biological Transport ◽  
Transport Pathway ◽  
Polystyrene Nanoparticles ◽  
Cell Monolayers ◽  
Intestinal Delivery ◽  
Cells Cultured

The aim of this study was to probe whether the transferrin (Tf) transport pathway can be exploited for intestinal delivery of nanoparticles. Tf was adsorbed on 100 nm model polystyrene nanoparticles (NP), followed by size characterisation of these systems. Cell uptake of Tf and Tf-adsorbed NP was investigated in intestinal epithelial Caco-2 cells cultured on multi-well plates and as differentiated polarised monolayers. Tf-NP demonstrated a remarkably higher cell uptake compared to unmodified NP in both non-polarised (5-fold) and polarised cell monolayers (16-fold difference). Application of soluble Tf significantly attenuated the uptake of Tf-NP. Notably, Tf-NP displayed remarkably higher rate (23-fold) of epithelial transport across Caco-2 monolayers compared to unmodified NP. This study therefore strongly suggests that the Tf transport pathway should be considered as a candidate biological transport route for orally-administered nanomedicines and drugs with poor oral bioavailability.

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