scholarly journals Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

2012 ◽  
pp. 3245 ◽  
Author(s):  
Asad Khan ◽  
Khan ◽  
Azam ◽  
Alam
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Therapeutic Approach ◽  
Novel Therapeutic

In vitro comparison of the effect of photodynamic therapy with curcumin and methylene blue on Candida albicans colonies

2019 ◽  
Vol 26 ◽  
pp. 193-198 ◽  
Author(s):  
Franak Daliri ◽  
Arash Azizi ◽  
Mehdi Goudarzi ◽  
Shirin Lawaf ◽  
Arash Rahimi
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
In Vitro Comparison

Synergic Effect of Photodynamic Therapy with Methylene Blue and Surfactants in the Inhibition of Candida albicans

2012 ◽  
Vol 175 (1-2) ◽  
pp. 159-164 ◽  
Author(s):  
Juliana Pereira Lyon ◽  
Rafael Reis Rezende ◽  
Mariana Penido Rabelo ◽  
Carlos José de Lima ◽  
Leonardo Marmo Moreira
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Synergic Effect

scholarly journals Biogenic Gold Nanoparticles Decrease Methylene Blue Photobleaching and Enhance Antimicrobial Photodynamic Therapy

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 623
Author(s):  
Irena Maliszewska ◽  
Ewelina Wanarska ◽  
Alex C. Thompson ◽  
Ifor D. W. Samuel ◽  
Katarzyna Matczyszyn
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Photodynamic Therapy ◽  
Bactericidal Effect ◽  
Antimicrobial Photodynamic Therapy ◽  
Plasmon Band ◽  
Escherichia Coli Bacteria ◽  
Average Size ◽  
20 Nm ◽  
Nanoparticle Sizes

Antibiotic resistance is a growing concern that is driving the exploration of alternative ways of killing bacteria. Here we show that gold nanoparticles synthesized by the mycelium of Mucor plumbeus are an effective medium for antimicrobial photodynamic therapy (PDT). These particles are spherical in shape, uniformly distributed without any significant agglomeration, and show a single plasmon band at 522–523 nm. The nanoparticle sizes range from 13 to 25 nm, and possess an average size of 17 ± 4 nm. In PDT, light (from a source consisting of nine LEDs with a peak wavelength of 640 nm and FWMH 20 nm arranged in a 3 × 3 array), a photosensitiser (methylene blue), and oxygen are used to kill undesired cells. We show that the biogenic nanoparticles enhance the effectiveness of the photosensitiser, methylene blue, and so can be used to kill both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. The enhanced effectiveness means that we could kill these bacteria with a simple, small LED-based light source. We show that the biogenic gold nanoparticles prevent fast photobleaching, thereby enhancing the photoactivity of the methylene blue (MB) molecules and their bactericidal effect.

scholarly journals Candida albicans Biofilm Inhibition by Ethnobotanicals and Ethnobotanically-Synthesized Gold Nanoparticles

2021 ◽  
Vol 12 ◽  
Author(s):  
Khristina G. Judan Cruz ◽  
Eleonor D. Alfonso ◽  
Somar Israel D. Fernando ◽  
Kozo Watanabe
Keyword(s):  
Gold Nanoparticles ◽  
Drug Resistance ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Inhibition Activity ◽  
Therapeutic Approaches ◽  
Biofilm Inhibition ◽  
Crude Extracts ◽  
Novel Therapeutic

The virulence and drug resistance of globally prevalent Candida albicans has presented complications toward its control while advances in effective antivirulence drugs remain critical. Emerging methods are now being evaluated to facilitate development of novel therapeutic approaches against this pathogen. This study focuses on the biofilm formation inhibition of ethnobotanical crude extracts and the use of nanotechnology through the ethnobotanically-synthesized gold nanoparticles to control C. albicans. Control on biofilm formation was compared using crude extracts (CEs) and biologically synthesized gold nanoparticles (CEs + AuNPs). Significantly lower biofilm formation was exhibited in thirteen (13) CEs and fourteen (14) CEs + AuNPs. Biofilm-linked genes Bcr1 and HSP90 expression were consequently downregulated. Higher biofilm inhibition activity was noted in some CEs + AuNPs compared to its counterpart CEs. This study emphasizes the biofilm inhibition activity of ethnobotanicals and the use of nanoparticles to enhance delivery of compounds, and points to its prospects for developing anti-pathogenic drugs without evolving resistance.

scholarly journals Photoinactivation of Candida albicans using methylene blue as photosensitizer

2015 ◽  
Vol 63 (4) ◽  
pp. 411-417 ◽  
Author(s):  
João Nilton Lopes de SOUSA ◽  
Bruna Honório de QUEIROGA ◽  
Patrícia de Oliveira KOCERGINSKY ◽  
Petrusk Homero Campos MARINHO ◽  
Ângela Toshie ARAKI
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Planktonic Cells ◽  
Power Laser ◽  
Antifungal Effect ◽  
Microtiter Plates ◽  
Colony Forming Units ◽  
Laser Control ◽  
Fungal Suspension

Objective: To evaluate the effect of photodynamic therapy in the inactivation of Candida albicans planktonic cells, using methylene blue, 150 mg/mL, as a photosensitizer. Methods: Aliquots of 100 (μg/mL of the fungal suspension in a 106 cells/mL concentration were seeded in microtiter plates with 96 wells, where the same volume of methylene blue was deposited, remaining for 5 min pre-irradiation. Then, the low power laser light was applied (wavelength: 660 nm, power: 100 mW and dose: 426 J/cm2) for 128s using a portable semiconductor laser. Control experiments were performed without illumination and in the absence of methylene blue, and another in the presence of methylene blue without illumination and with illumination, replacing the photosensitizer by saline. In each experimental condition, serial dilutions (10-1 to 10-3) were obtained and 25 μL aliquots were seeded in Sabouraud Dextrose Agar duplicate. After this period, the number of colony forming units per milliliter (CFU/mL) was determined and the data were submitted to variance analysis and Kruskal Wallis test (p <0.05). Results: At the concentration of 150 μg/mL, and time of 5 min incubation, the reduction was significant (p˂0.05). In the absence of irradiation, methylene blue produced no reduction in CFU/mL. Conclusion: Photodynamic Therapy presented antifungal effect against Candida albicans and can be used as an adjunct to conventional treatment.

scholarly journals Photodynamic Eradication of Trichophyton rubrum and Candida albicans

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 263
Author(s):  
Anton Valkov ◽  
Michael Zinigrad ◽  
Marina Nisnevitch
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Trichophyton Rubrum ◽  
Nail Plate ◽  
Correct Choice ◽  
Individual Response ◽  
Glycerol Water ◽  
New Methods ◽  
Malachite Green Oxalate

Conventional methods of onychomycosis treatment are ineffective in some cases because the cure of onychomycosis very often depends on the patient’s individual response to the treatment; therefore, there is a crucial need to research and develop new methods of onychomycosis therapy. One of the most innovative treatments is photodynamic therapy (PDT) using photosensitizers (PSs). However, effective treatment depends on the correct choice of photosensitizer and substances that improve the characteristics of the final formulation. The aim of our work was to find an effective formulation for the treatment of onychomycosis. To achieve this goal, we tested the effect of three types of PSs, rose Bengal (RB), malachite green oxalate (MGO), and methylene blue (MB), on Candida albicans. The most effective PS was RB, and so the study was continued with Trichophyton rubrum. Additional comparative studies were carried out on substances included in the formulation (urea and thiourea), focusing on their antifungal activity, which can improve penetration through the nail plate. The composition of the formulation that achieved 100% eradication of Trichophyton rubrum under our conditions consisted of 150 μM RB, 5% urea, and 0.5% thiourea in glycerol/water (70/30%, w/w) solution. A white luminescent lamp was used as a light source (1.9 ± 0.1 mW cm−2). Stability of the formulation was checked. The selected formulation shows potential for future simplification and acceleration of PDT treatment of onychomycosis.

scholarly journals Photodynamic Inactivation of Candida albicans in Blood Plasma and Whole Blood

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 221 ◽  
Author(s):  
Vera Sousa ◽  
Ana T. P. C. Gomes ◽  
Américo Freitas ◽  
Maria A. F. Faustino ◽  
Maria G. P. M. S. Neves ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Blood Plasma ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Antimicrobial Photodynamic Therapy ◽  
Photodynamic Inactivation ◽  
Blood Components

The few approved disinfection techniques for blood derivatives promote damage in the blood components, representing risks for the transfusion receptor. Antimicrobial photodynamic therapy (aPDT) seems to be a promising approach for the photoinactivation of pathogens in blood, but only three photosensitizers (PSs) have been approved, methylene blue (MB) for plasma and riboflavin and amotosalen for plasma and platelets. In this study, the efficiency of the porphyrinic photosensitizer Tri-Py(+)-Me and of the porphyrinic formulation FORM was studied in the photoinactivation of Candida albicans in plasma and in whole blood and the results were compared to the ones obtained with the already approved PS MB. The results show that FORM and Tri-Py(+)-Me are promising PSs to inactivate C. albicans in plasma. Although in whole blood the inactivation rates obtained were higher than the ones obtained with MB, further improvements are required. None of these PSs had promoted hemolysis at the isotonic conditions when hemolysis was evaluated in whole blood and after the addition of treated plasma with these PSs to concentrates of red blood cells.

The Synthesis of Methylene Blue Photosensitiser Conjugated with Gold Nanoparticles

2013 ◽  
Vol 829 ◽  
pp. 299-303 ◽  
Author(s):  
Hasan Kariminezhad ◽  
Sona Khayat Omrani ◽  
Setareh Habibzadeh ◽  
Maria Agheb
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Photodynamic Therapy ◽  
Infrared Spectroscopy ◽  
Cellular Uptake ◽  
Force Microscopy ◽  
Atomic Force ◽  
Uv Visible

Nowadays, nanocarriers possess growing interest for medical usage such as photodynamic therapy. Conjunction of these nanoparticles with photosensitising drugs leads to more cellular uptake, higher photocytotoxicty, higher biocompatibility and more selectivity. Previous papers conclude that the 50 nm size nanoparticles give the highest cellular uptake for the purpose of photodynamic therapy. In this paper the gold nanoparticles were synthesized based on TurkevichFrens method. Moreover, methylene blue as a relevant photosensitiser was conjugated to achieved AuNPs. The spectrometric results of UV-visible and Fourier Transform Infrared spectroscopy (FTIR) as well as Atomic Force Microscopy (AFM) emphasis on the presence of MB sensitizers connected to resulting 50 nm gold nanoparticles.

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