scholarly journals Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

2012 ◽  
pp. 4335 ◽  
Author(s):  
Hsiu-Chen Lin ◽  
Herng-Er Horng ◽  
Yang ◽  
Hsiu-Chen Lin
Keyword(s):  
Clinical Samples ◽  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein

One-step homogeneous C-reactive protein assay for saliva

2011 ◽  
Vol 373 (1-2) ◽  
pp. 19-25 ◽  
Author(s):  
Chamindie Punyadeera ◽  
Goce Dimeski ◽  
Karam Kostner ◽  
Peter Beyerlein ◽  
Justin Cooper-White
Keyword(s):  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein ◽  
One Step

scholarly journals Ultrarapid, Ultrasensitive One-Step Kinetic Immunoassay for C-Reactive Protein (CRP) in Whole Blood Samples: Measurement of the Entire CRP Concentration Range with a Single Sample Dilution

2002 ◽  
Vol 48 (2) ◽  
pp. 269-277 ◽  
Author(s):  
Piia Tarkkinen ◽  
Tom Palenius ◽  
Timo Lövgren
Keyword(s):  
Whole Blood ◽  
Solid Phase ◽  
Point Of Care ◽  
High Sensitivity ◽  
Clinical Samples ◽  
Cardiac Complications ◽  
Fluorescence Signal ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
One Step

Abstract Background: Recently, measurement of very low concentrations of C-reactive protein (CRP) has gained popularity as a potential new means for predicting the risk of future cardiac complications. In this study, we demonstrate the feasibility of a kinetic, one-step microparticle assay for quantitative determination of extremely low and high CRP concentrations in the limited timeframe typical for point-of-care testing. Methods: A noncompetitive, kinetic CRP immunoassay was developed that uses individual, porous microparticles as the solid phase. The microparticles were covalently coated with a monoclonal capture antibody, and the monoclonal detection antibody was labeled with europium. The one-step binding reaction was stopped by washing after 2 min of incubation, and the fluorescence signal of individual particles was measured. Results: The analytical detection limit (mean of zero calibrator + 3 SD) was 0.00016 mg/L CRP. Clinical samples were diluted 400-fold before assay to cover the CRP concentration range of 0.064–1200 mg/L. The assay correlated well with the Dade Behring N High Sensitivity CRP assay (for 0–10 mg/L, r = 0.969, Sy|x = 0.68, n = 54; for 0–350 mg/L, r = 0.969, Sy|x = 11.7, n = 100). The within- and between-run CVs based on calculated concentrations were, respectively, 9–16% and 14% at 0.11 mg/L, 4.5–12% and 8.2% at 4.2 mg/L, and 3.5–6.3% and 4.4% at 105 mg/L, with a CV <15% at 0.2 mg/L and above. Conclusions: Use of the kinetic microparticle approach combined with time-resolved fluorometry allows ultrasensitive quantification of CRP in whole blood in 2 min with a linear assay range spanning more than four orders of magnitude.

scholarly journals C-reactive protein assay in obese paediatric patients: comparison of two laboratory methods

2015 ◽  
Author(s):  
Jose Alves ◽  
Marta Manacas ◽  
Carolina Faria ◽  
Lia Filipe ◽  
Susana Silva ◽  
...  
Keyword(s):  
C Reactive Protein ◽  
Laboratory Methods ◽  
Protein Assay ◽  
Reactive Protein ◽  
Paediatric Patients

Serum Free Light Chain Assay: Shift Toward a Higher κ/λ Ratio

2019 ◽  
Vol 5 (1) ◽  
pp. 114-125 ◽  
Author(s):  
Barbara Rindlisbacher ◽  
Christof Schild ◽  
Florence Egger ◽  
Vera U Bacher ◽  
Thomas Pabst ◽  
...  
Keyword(s):  
Clinical Samples ◽  
Poor Correlation ◽  
Poor Agreement ◽  
C Reactive Protein ◽  
Serum Free ◽  
Reactive Protein ◽  
Serum Free Light Chain ◽  
Academic Center ◽  
Plasma Cell Disorders ◽  
Serum Free Light Chains

Abstract Background The analysis of serum free light chains (FLCs) is clinically relevant for the diagnosis and therapeutic management of clonal plasma cell disorders. This study compares the performance of monoclonal and polyclonal FLC κ and λ assays in clinical samples determined in a single academic center. Methods Serum FLCs were analyzed from 102 patients using the Freelite (Binding Site) and N Latex (Siemens) assays on the BN ProSpec System (Siemens). When available, data for protein electrophoresis, immunofixation, C-reactive protein, and estimated glomerular filtration rate (eGFR) were combined with FLC results to evaluate performance. Results Method evaluation showed acceptable imprecision and inaccuracy measures of <4.4% and 12.9%, respectively. Poor agreement between the methods was observed, including constant and proportional bias and poor correlation (Kendall τ, 0.671–0.901). The N Latex assay was not affected by the renal impairment estimated by eGFR, unlike the FLC κ/λ ratio results by the Freelite assay. With the Freelite assay, 98% of putative controls without monoclonal gammopathy (n = 42) showed a κ/λ ratio that was above the median of the standard diagnostic range or renal diagnostic range. A shift toward higher κ/λ ratios was also observed when retrospective data between 2011 and 2017 were compared. Conclusions Unlike the Freelite assay, κ/λ ratios analyzed with the N Latex assay were not affected by renal failure. Both methods showed acceptable performances using nephelometry, but they were poorly correlated. A shift toward κ/λ ratios might impair the specificity of borderline increased κ/λ results. This should be considered when interpreting FLC κ and λ results.

Performance characteristics of a point of care C-reactive protein assay

2001 ◽  
Vol 314 (1-2) ◽  
pp. 255-259 ◽  
Author(s):  
William L Roberts ◽  
Elisabeth L Schwarz ◽  
Shake Ayanian ◽  
Nader Rifai
Keyword(s):  
Point Of Care ◽  
Performance Characteristics ◽  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein

scholarly journals Validation and application of a canine-specific automated high-sensitivity C-reactive protein assay

2015 ◽  
Vol 27 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Anna Hillström ◽  
Ragnvi Hagman ◽  
Josefin Söder ◽  
Jens Häggström ◽  
Ingrid Ljungvall ◽  
...  
Keyword(s):  
High Sensitivity ◽  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein

scholarly journals An electrochemical immunosensor coupling a bamboo-like carbon nanostructure substrate with toluidine blue-functionalized Cu(ii)-MOFs as signal probes for a C-reactive protein assay

RSC Advances ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 6699-6708 ◽  
Author(s):  
Mei Li ◽  
Xiaojuan Xia ◽  
Shuang Meng ◽  
YuChan Ma ◽  
Tong Yang ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Metal Organic Framework ◽  
Electrochemical Immunosensor ◽  
C Reactive Protein ◽  
Carbon Nanostructure ◽  
Protein Assay ◽  
Nitrogen Doped ◽  
Reactive Protein ◽  
Metal Organic ◽  
Organic Framework

A sandwich immunosensor based on a toluidine blue (Tb) loaded metal organic framework (Cu(ii)-HKUST-1/Tb) as the signal label and a nitrogen-doped 3D carbon nanostructure as the immobilizing matrix was constructed for the detection of C-reactive protein.

Sign in / Sign up

Export Citation Format

Share Document