scholarly journals Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay

2020 ◽  
Vol Volume 15 ◽  
pp. 9975-9985
Author(s):  
Jin Man ◽  
Jiajia Dong ◽  
Yilin Wang ◽  
Leiliang He ◽  
Songcheng Yu ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Time Resolved ◽  
Aptamer Assay

Development of a Dual-Label Time-Resolved Fluorometric Immunoassay for the Simultaneous Detection of Two Recombinant Proteins in Potato

2000 ◽  
Vol 48 (12) ◽  
pp. 5868-5873 ◽  
Author(s):  
Jeffrey T. Bookout ◽  
Tony R. Joaquim ◽  
Kimberly M. Magin ◽  
Glennon J. Rogan ◽  
Ronald P. Lirette
Keyword(s):  
Recombinant Proteins ◽  
Simultaneous Detection ◽  
Time Resolved ◽  
Dual Label ◽  
Label Time

scholarly journals Automated Time-resolved Immunofluorometric Assay for Toxoplasma gondii-specific IgM and IgA Antibodies: Study of More Than 130 000 Filter-Paper Blood-Spot Samples from Newborns

2002 ◽  
Vol 48 (11) ◽  
pp. 1981-1986 ◽  
Author(s):  
Tina Sørensen ◽  
Johanne Spenter ◽  
Irakli Jaliashvili ◽  
Michael Christiansen ◽  
Bent Nørgaard-Pedersen ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Filter Paper ◽  
Simultaneous Detection ◽  
Congenital Toxoplasmosis ◽  
Comparison Method ◽  
Time Resolved ◽  
Iga Antibodies ◽  
Reference Serum ◽  
Filter Paper Blood Spot ◽  
Blood Spot

Abstract Background: To screen for congenital toxoplasmosis, we developed a time-resolved immunofluorometric assay for the simultaneous detection of Toxoplasma gondii-specific IgM and IgA in filter-paper samples collected from newborns 4–7 days after birth. Methods: The assay was performed on the AutoDELFIATM, and results were calculated based on the ToxoM WHO Third International Reference Serum. Comparison with an in-house μ-capture immunoassay was carried out retrospectively on filter-paper samples from children with confirmed congenital toxoplasmosis. Prospectively the assay was compared with a μ-capture immunoassay on 68 394 samples and a commercially available assay on another 69 467 samples. Before serum was requested from the newborn, positive samples were tested for IgA and IgM separately and in an IgM-immunosorbent agglutination assay developed for filter-paper samples. Results: Intra- and interassay variations (CVs) were 8% and 16%, respectively. The cutoff of 5 units/mL produced a 0.5% retest rate. The assay detected 13 of 18 (72%) samples from newborns diagnosed with congenital toxoplasmosis in the retrospective study. Prospectively, the assay identified 24 newborns who were later diagnosed with congenital toxoplasmosis. Results for all 24 cases were positive by the respective comparison method. No cases were detected solely by the IgA antibodies in the sample. Conclusion: Neonatal screening for congenital toxoplasmosis can be automated by use of purified europium-labeled antigen for detection of T. gondii-specific IgM and IgA eluted from filter-paper samples.

Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay

2015 ◽  
Vol 22 (19) ◽  
pp. 14882-14890 ◽  
Author(s):  
Haiyan Shi ◽  
Enze Sheng ◽  
Lu Feng ◽  
Liangliang Zhou ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Time Resolved

Simultaneous detection of Mycoplasma pneumoniae IgG and IgM using dual-label time resolved fluoroimmunoassay

2018 ◽  
Vol 548 ◽  
pp. 1-6 ◽  
Author(s):  
Yi Zhang ◽  
Xue Yang ◽  
Jun Qian ◽  
Xiaohong Gu ◽  
Jue Zhang ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Simultaneous Detection ◽  
Time Resolved ◽  
Dual Label ◽  
Label Time
Sign in / Sign up

Export Citation Format

Share Document