scholarly journals Controlled release of paclitaxel from a self-assembling peptide hydrogel formed in situ and antitumor study in vitro

2011 ◽  
pp. 2143 ◽  
Author(s):  
Jingping Liu
Keyword(s):  
Controlled Release ◽  
Self Assembling ◽  
Peptide Hydrogel

scholarly journals The interaction between self – assembling peptides and emodin and the controlled release of emodin from in-situ hydrogel

2019 ◽  
Vol 47 (1) ◽  
pp. 3961-3975 ◽  
Author(s):  
Weipeng Wei ◽  
Cui Meng ◽  
Yuhe Wang ◽  
Yongsheng Huang ◽  
Wenbin Du ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembling ◽  
In Situ Hydrogel

scholarly journals Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

2016 ◽  
Vol Volume 10 ◽  
pp. 3043-3051 ◽  
Author(s):  
Ao Zhou ◽  
Shuo Chen ◽  
Bin He ◽  
Weikang Zhao ◽  
Xiaojun Chen ◽  
...  
Keyword(s):  
Controlled Release ◽  
Tgf Beta ◽  
Hydrogel Scaffolds ◽  
Self Assembling ◽  
Peptide Hydrogel

scholarly journals Self-assembling peptides and their applications in tissue engineering

2018 ◽  
Author(s):  
Graziano Deidda
Keyword(s):  
Tissue Engineering ◽  
Direct Laser Writing ◽  
Laser Writing ◽  
Self Assembling ◽  
Direct Laser

Τα πρωτεϊνικά ικριώματα τα οποία σχηματίζονται από αυτοοργανωμένα πεπτίδια μπορούν να χρησιμοποιηθούν ως εναλλακτικά των πρωτεϊνών της εξωκυττάριας μήτρας (ECM) και θα μπορούσαν να βρουν εφαρμογή στην αναγεννητική ιατρική. Στο πρώτο κεφάλαιο της παρούσας διατριβής ένα πεπτίδιο που περιέχει την αμυλοειδή αλληλουχία NSGAITIG η οποία έχει ανακτηθεί από το μίσχο της πρωτεΐνης, ίνας του αδενοϊού ανασχεδιάστηκε για την κατασκευή τρισδιάστατων (3D) ικριωμάτων ανθρώπινου ιστού και μοντέλων κυτταρικής καλλιέργειας in vitro. Εν συνεχεία αυτή η ακολουθία μελετήθηκε έτσι ώστε να διαμορφωθεί ένα εντεκαπεπτίδιο το οποίο περιέχει το μοτίβο RGD στο αμινοτελικό άκρο και ένα κομβικό αμινοξύ όπως η κυστεΐνη στο καρβοξυτελικό άκρο, έτσι ώστε να σχηματιστεί η αλληλουχία RGDSGAITIGC ως τελικό προϊόν. Το αμυλοειδές αυτό πεπτίδιο μπορεί να παίξει έναν πολλά υποσχόμενο διλειτουργικό ρόλο ως βιολογικό ικρίωμα καθώς επάγει την κυτταρική προσκόλληση και λόγω του αμινοξέα της κυστεΐνης μπορεί να χρησιμοποιηθεί ως ενδιάμεσος προσδέτης βιομορίων. Εναλλακτικά, στο δεύτερο κεφάλαιο, φωτοευαίσθητα κατάλοιπα όπως η τυροσίνη ή η τρυπτοφάνη σχεδιάστηκαν για να εισαχθούν στο καρβοξυτελικό άκρο ώστε να δώσουν τις αλληλουχίες RGDSGAITIGY και RGDSGAITIGW. Αυτά τα κατάλοιπα μπορούν να πυροδοτήσουν ένα φαινόμενο πολυφωτονικού πολυμερισμού (MPP/MPA) μέσω της χρήσης της τεχνικής της Direct Laser Writing (DLW) με απώτερο στόχο την εφαρμογή σε υψηλά ανταποκρινόμενες βιολογικές θεραπείες, όπως η αναγέννηση του μυοκαρδίου. Εν συνεχεία, στο τρίτο κεφάλαιο, τα πεπτιδικά παράγωγα τα οποία χαρακτηρίστηκαν προηγουμένως, μελετήθηκαν ως υποστρώματα στη θεραπεία βλαστοκυττάρων. Επιπλέον τροποποιημένα εμβρυϊκά βλαστοκύτταρα μελετήθηκαν για κυτταρική προσκόλληση και πολλαπλασιασμό. Επιπροσθέτως, η μελέτη των ρεολογικών ιδιοτήτων όλων των υποστρωμάτων και η περαιτέρω αξιολόγηση της λειτουργικής έγχυσης μελετήθηκε με σκοπό την προοπτική θεραπευτικής χρησιμοποίησής τους in situ.

Targeted Chemotherapy for Breast Cancer Using an Intelligent Doxorubicin-Loaded Hexapeptide Hydrogel

2020 ◽  
Vol 16 (6) ◽  
pp. 842-852
Author(s):  
Shiyao Luo ◽  
Ying Zhu ◽  
Yiping Li ◽  
Li Chen ◽  
Shunzhong Lv ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Solid Phase ◽  
Synthesis Method ◽  
High Water ◽  
High Water Content ◽  
Normal Tissues ◽  
Self Assembling ◽  
Peptide Hydrogel

Self-assembling peptide hydrogels have a high water content, good biocompatibility and have become a competitive research object in the fields of tissue engineering, cancer treatment and drug delivery. In our research, a hexapeptide with high pH sensitivity was designed and synthesized by utilizing a solid-phase synthesis method. Under physiological conditions, the peptide could self-assemble into a hydrogel. When it reached the tumor acidic microenvironment, the peptide was degraded and doxorubicin was released to exert its antitumor effect. A series of physicochemical properties were investigated, including gelling ability, secondary structure, micromorphology, rheological properties and drug release studies. The results illustrated that PIDO peptide hydrogel has good pH responsiveness and injectability. In vitro cytotoxicity experiments and in vivo antitumor experiments showed that PIDO peptide hydrogel has a highly effective therapeutic effect on tumor cells and is less toxic to normal tissues. Our research provides a promising option for targeted drug delivery and sustainable release.

scholarly journals Study of the interaction between self-assembling peptide and mangiferin and in vitro release of mangiferin from in situ hydrogel

2019 ◽  
Vol Volume 14 ◽  
pp. 7447-7460 ◽  
Author(s):  
Cui Meng ◽  
Weipeng Wei ◽  
Yuhe Wang ◽  
Kunqin Zhang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
In Vitro Release ◽  
Self Assembling ◽  
In Situ Hydrogel ◽  
Vitro Release

scholarly journals Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System

2020 ◽  
Vol 21 (12) ◽  
pp. 4261
Author(s):  
Raffaele Pugliese ◽  
Fabrizio Gelain
Keyword(s):  
Mechanical Properties ◽  
Self Assembly ◽  
Self Healing ◽  
One Pot ◽  
Self Assembling ◽  
In Situ Gelation ◽  
Wide Range ◽  
Peptide Hydrogels ◽  
Peptide Hydrogel

Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo–NHS) coupling, to tune its biomechanics. EDC/sulfo–NHS coupling led to limited changes in storage modulus (from 0.9 to 2 kPa), but it significantly increased both the strain (from 6% to 60%) and failure stress (from 19 to 35 Pa) of peptide hydrogel without impairing the spontaneous formation of β-sheet-containing nano-filaments. Furthermore, EDC/sulfo–NHS cross-linking bestowed self-healing and thixotropic properties to the peptide hydrogel. Lastly, we demonstrated that this strategy can be used to incorporate bioactive functional motifs after self-assembly on pre-formed nanostructures by functionalizing an Ac-LDLKLDLKLDLK-CONH2 (LDLK12) self-assembling peptide with the phage display-derived KLPGWSG peptide involved in the modulation of neural stem cell proliferation and differentiation. The incorporation of a functional motif did not alter the peptide’s secondary structure and its mechanical properties. The work reported here offers new tools to both fine tune the mechanical properties of and tailor the biomimetic properties of self-assembling peptide hydrogels while retaining their nanostructures, which is useful for tissue engineering and regenerative medicine applications.

Facile in situ growth of Fe3O4 nanoparticles on hydroxyapatite nanorods for pH dependent adsorption and controlled release of proteins

RSC Advances ◽  
2014 ◽  
Vol 4 (92) ◽  
pp. 50510-50520 ◽  
Author(s):  
G. Bharath ◽  
D. Prabhu ◽  
D. Mangalaraj ◽  
C. Viswanathan ◽  
N. Ponpandian
Keyword(s):  
Controlled Release ◽  
Protein Adsorption ◽  
Fe3o4 Nanoparticles ◽  
In Situ Growth ◽  
Hydrothermal Technique ◽  
Ph Dependent

The magnetic hydroxyapatite nanostructures were prepared by hydrothermal technique and studied their protein adsorption and in vitro cytotoxicity in humen MGC-803 cell.

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