scholarly journals Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects

2020 ◽  
Vol Volume 15 ◽  
pp. 3039-3056 ◽  
Author(s):  
Hongbin Zhao ◽  
Junjie Tang ◽  
Dong Zhou ◽  
Yiping Weng ◽  
Wen Qin ◽  
...  
Keyword(s):  
Bone Defects ◽  
Core Shell ◽  
Composite Scaffolds ◽  
Tibia Bone ◽  
Hydroxyapatite Composite ◽  
Rabbit Tibia

scholarly journals Evaluation of Vertical Guided Bone Regeneration Using a Particulate Form of Experimental Bioactive Glass in a Rabbit: A Case Report with Literature Review

2021 ◽  
Vol 11 (3) ◽  
pp. 308-314
Author(s):  
Timur V. Melkumyan ◽  
Nuritdin Kh. Kamilov ◽  
Fatima Yu. Daurova ◽  
Angela D. Dadamova
Keyword(s):  
Bioactive Glass ◽  
Soft Tissues ◽  
Surgical Techniques ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Tibia Bone ◽  
Barrier Membrane ◽  
Dental Implantation ◽  
Rabbit Tibia ◽  
Osteoplastic Material

Background: There are a large number of different types of bone-grafting materials that are used for the regeneration of atrophied alveolar ridges in order to make dental implantation possible. However, available surgical techniques and materials for bone augmentation do not contribute to the achievement of the desired reliable results and require a search for new solutions to an existing problem. A group of synthetic osteoplastic materials based on bioactive glass (BAG) may become a matter of choice in bone tissue regeneration because of special osteogenic properties. The aim of this study was to visually and histologically evaluate the behavior of an experimental BAG in rabbit tibia bone samples, which were collected from the animal 6 weeks after filling the bone defects. Methods and Results: The observation was carried out on one outbred rabbit whose tibia bone defects were filled with an experimental osteoplastic material based on the BAG. The chemical composition of the experimental osteoplastic material included SiO2 (41%), Na2O (21%), CaO (28.5%), P2O5 (6%), CaF2 (1.5%), MgO (1%), Al2O3 (1%). For histological analysis, H&E staining of paraffin-embedded tissues was performed according to the standard technique. Light microscopy of tissue samples was performed using a Leitz HM-LUX microscope (Germany). Six weeks after filling the bone defects, a strong bond between the augmented hard tissue and rabbit tibia was recognized. Also, a dense fusion of adjacent soft tissues with a newly formed bone without signs of chronic inflammation or graft particles in granular tissue was noted. Microscopic examination of the stained sections showed the presence of mature viable BT with a uniform distribution of osteocytes. Also, residual fragments of the degraded biomaterial surrounded by the fibers of a woven bone were revealed in several slices. Conclusion: In accordance with the results of this experiment, it can be concluded that the usage of BAG related to the system SiO2(41%)-Na2O(21%)-CaO(28.5%)-P2O5(6%)-CaF2(1.5%)-MgO(1%)-Al2O3(1%) may increase the volume of bone without application of barrier membrane. However, further research involving more animals needs to be done to estimate the scientific significance of the obtained data and to evaluate the mechanical properties of augmented bone.

Effect of mesoporous silica and its combination with hydroxyapatite on the regeneration of rabbit’s bone defects: A pilot study

2021 ◽  
pp. 1-14
Author(s):  
Mario Pérez Sayans ◽  
Berta Rivas Mundiña ◽  
Cintia M. Chamorro Petronacci ◽  
Abel García García ◽  
Francisco José Gómez García ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Surgical Techniques ◽  
Biological Response ◽  
Bone Defects ◽  
Powdered Material ◽  
Histopathological Study ◽  
Radiographic Images ◽  
Osteogenic Response ◽  
Rabbit Tibia ◽  
Hybrid Scaffolds

BACKGROUND: Bone volume augmentation is a routine technique used in oral implantology and periodontology. Advances in the surgical techniques and the biomaterials field have allowed a greater accessibility to these treatments. Nevertheless, dehiscence and fenestrations incidence during dental implant procedures are still common in patients with bone loss. OBJECTIVE: The main objective is to evaluate in a pilot experimental study the biological response to mesoporous silica (MS) hybrid scaffolds and its regenerative capacity in different formulations. METHODS: Two defects per rabbit tibia were performed (one for control and other for test) and the biomaterials tested in this study have been used to fill the bone defects, prepared in two different formulations (3D hybrid scaffolds or powdered material, in 100% pure MS form, or 50% MS with 50% hydroxyapatite (HA). Euthanasia was performed 4 months after surgery for bone histopathological study and radiographic images were acquired by computerized microtomography. RESULTS: Results showed that radiographically and histopathologically pure MS formulations lead to a lower biological response, e.g when formulated with HA, the osteogenic response in terms of osteoconduction was greater. CONCLUSIONS: We observed tolerance and lack of toxicity of the MS and HA, without registering any type of local or systemic allergic reaction.

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

Composite Scaffolds for Large Bone Defects

2019 ◽  
pp. 250-257
Author(s):  
Evangelos Daskalakis ◽  
Enes Aslan ◽  
Fengyuan Liu ◽  
Glen Cooper ◽  
Andrew Weightman ◽  
...  
Keyword(s):  
Bone Defects ◽  
Composite Scaffolds ◽  
Large Bone ◽  
Large Bone Defects

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals Fabrication and Characterization of Ta–GelMA–BG Scaffolds by Chemical Crosslinking Processing for Promotion Osteointegration

2021 ◽  
Vol 8 ◽  
Author(s):  
Fujian Zhao ◽  
Xiongfa Ji ◽  
Yang Yan ◽  
Zhen Yang ◽  
Xiaofeng Chen ◽  
...  
Keyword(s):  
Early Stage ◽  
Bone Defects ◽  
Chemical Crosslinking ◽  
Composite Scaffolds ◽  
Load Bearing ◽  
3D Printed ◽  
Fabrication And Characterization ◽  
Cell Adhesion And Proliferation

The repair of bone defects in load-bearing positions still faces great challenges. Tantalum (Ta) has attempted to repair bone defects based on the excellent mechanical properties. However, the osseointegration of Ta needs to be improved due to the lack of osteoinduction. Herein, tantalum–gelatin–methacryloyl–bioactive glass (Ta–GelMA–BG) scaffolds were successfully fabricated by loading BG in 3D-printed Ta scaffolds through a chemical crosslinking method. The results showed that the composite scaffolds have the ability to promote cell adhesion and proliferation. The incorporation of BG resulted in a significant increase in apatite-forming and osteogenesis differentiation abilities. In vivo results indicated that the Ta–GelMA–BG scaffolds significantly enhanced the osteointegration at the early stage after implantation. Overall, the Ta–GelMA–BG scaffolds are a promising platform for the load bearing bone regeneration field.

scholarly journals Comparison of Ex-Vivo X-Ray changes in different groups of injectable biomaterials (JectOS, MIIG®X3, Osteopaste) in critical size defect of Rabbit Tibia Bone

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Wan Maihan Wan Salleh ◽  
Mohamed Azril Mohamed Amin ◽  
Wan Muhamad Salahudin Wan Salleh ◽  
Zunariah Buyong
Keyword(s):  
Critical Size ◽  
Ex Vivo ◽  
Control Group ◽  
Sham Operation ◽  
Critical Size Defect ◽  
X Ray ◽  
Tibia Bone ◽  
Local Product ◽  
Rabbit Tibia ◽  
Radiological Changes

Introduction: Critical size defect (CSD) is defined as a defect that will not heal without intervention within the lifetime. The gold standard treatment for CSD is bone graft, although with some limitations. Substitute biomaterials were introduced to overcome the limitations. JectOS and MIIG® X3 are commercially available biomaterials in the market. Osteopaste is a local product produced by SIRIM. The objective of this study is to compare the radiological changes between Osteopaste, JectOS and MIIG® X3 in CSD in rabbit tibia bone. Methods: New Zealand White rabbits were divided into four groups: control group (Sham operation, n=3); Osteopaste treatment (n=12); JectOS treatment (n=12) and MIIG® X3 treatment (n=12). CSD was created at the right proximal tibia bone of the rabbits in each of the groups and the defects were filled with the biomaterial as assigned. Four animals from each group were sacrificed at 6 weeks, 12 weeks and 24 weeks respectively. The bones were harvested and x-ray imaging performed using SkyScan 1176 at 90kV, 281µA, resolution 4000x2672 with Aluminium 1.0mm. Results: The radiographic density at the CSD area was more prominent in the JectOS group throughout the 24 weeks. Meanwhile, in the MIIG® X3, full resorption occurred at 24 weeks. The Osteopaste group exhibited radiographic density in between that of JectOS and MIIG® X3. Conclusions: Different types of biomaterial exhibit different radiological changes over the period of bone healing.

scholarly journals Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold

2018 ◽  
Vol 7 (1) ◽  
pp. 46-57 ◽  
Author(s):  
J. Zhou ◽  
X. G. Zhou ◽  
J. W. Wang ◽  
H. Zhou ◽  
J. Dong
Keyword(s):  
Controlled Release ◽  
Tricalcium Phosphate ◽  
Chronic Osteomyelitis ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Therapeutic Drug ◽  
Porous Scaffolds ◽  
Composite Scaffolds ◽  
Extended Duration ◽  
Thorough Debridement

Objective In the present study, we aimed to assess whether gelatin/β-tricalcium phosphate (β-TCP) composite porous scaffolds could be used as a local controlled release system for vancomycin. We also investigated the efficiency of the scaffolds in eliminating infections and repairing osteomyelitis defects in rabbits. Methods The gelatin scaffolds containing differing amounts of of β-TCP (0%, 10%, 30% and 50%) were prepared for controlled release of vancomycin and were labelled G-TCP0, G-TCP1, G-TCP3 and G-TCP5, respectively. The Kirby-Bauer method was used to examine the release profile. Chronic osteomyelitis models of rabbits were established. After thorough debridement, the osteomyelitis defects were implanted with the scaffolds. Radiographs and histological examinations were carried out to investigate the efficiency of eliminating infections and repairing bone defects. Results The prepared gelatin/β-TCP scaffolds exhibited a homogeneously interconnected 3D porous structure. The G-TCP0 scaffold exhibited the longest duration of vancomycin release with a release duration of eight weeks. With the increase of β-TCP contents, the release duration of the β-TCP-containing composite scaffolds was decreased. The complete release of vancomycin from the G-TCP5 scaffold was achieved within three weeks. In the treatment of osteomyelitis defects in rabbits, the G-TCP3 scaffold showed the most efficacious performance in eliminating infections and repairing bone defects. Conclusions The composite scaffolds could achieve local therapeutic drug levels over an extended duration. The G-TCP3 scaffold possessed the optimal porosity, interconnection and controlled release performance. Therefore, this scaffold could potentially be used in the treatment of chronic osteomyelitis defects. Cite this article: J. Zhou, X. G. Zhou, J. W. Wang, H. Zhou, J. Dong. Treatment of osteomyelitis defects by a vancomycin-loaded gelatin/β-tricalcium phosphate composite scaffold. Bone Joint Res 2018;7:46–57. DOI: 10.1302/2046-3758.71.BJR-2017-0129.R2.

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