scholarly journals Novel Aptamer-Functionalized Nanoparticles Enhances Bone Defect Repair By Improving Stem Cell Recruitment

2019 ◽  
Vol Volume 14 ◽  
pp. 8707-8724 ◽  
Author(s):  
Meng Wang ◽  
Haibin Wu ◽  
Qiao Li ◽  
Ying Yang ◽  
Fengyu Che ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Defect ◽  
Functionalized Nanoparticles ◽  
Cell Recruitment ◽  
Bone Defect Repair ◽  
Defect Repair

scholarly journals Novel Aptamer-Functionalized Nanoparticles Enhances Bone Defect Repair by Improving Stem Cell Recruitment [Corrigendum]

2020 ◽  
Vol Volume 15 ◽  
pp. 6093-6094
Author(s):  
Meng Wang ◽  
Haibin Wu ◽  
Qiao Li ◽  
Ying Yang ◽  
Fengyu Che ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Defect ◽  
Functionalized Nanoparticles ◽  
Cell Recruitment ◽  
Bone Defect Repair ◽  
Defect Repair

scholarly journals Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Francesca Diomede ◽  
Agnese Gugliandolo ◽  
Paolo Cardelli ◽  
Ilaria Merciaro ◽  
Valeria Ettorre ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Defect ◽  
Extracellular Vesicles ◽  
Three Dimensional ◽  
Bone Defect Repair ◽  
Defect Repair

Critical-size bone defect repair using amniotic fluid stem cell/collagen constructs: Effect of oral ferutinin treatment in rats

Life Sciences ◽  
2015 ◽  
Vol 121 ◽  
pp. 174-183 ◽  
Author(s):  
Manuela Zavatti ◽  
Laura Bertoni ◽  
Tullia Maraldi ◽  
Elisa Resca ◽  
Francesca Beretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Bone Defect ◽  
Critical Size ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Amniotic Fluid Stem Cell

Stem cell-seeded 3D-printed scaffolds combined with self-assembling peptides for bone defect repair

2021 ◽  
Author(s):  
Haixia Xu ◽  
Chengqiang Wang ◽  
Chun Liu ◽  
Jianjun Li ◽  
Ziyue Peng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Defect ◽  
Bone Defect Repair ◽  
Self Assembling ◽  
Defect Repair ◽  
3D Printed

scholarly journals Mitochondria transfer enhances bone marrow mesenchymal stem cell functions and promotes bone defect healing

2020 ◽  
Author(s):  
Yusi Guo ◽  
Xiaopei Chi ◽  
Yifan Wang ◽  
Boon Chin Heng ◽  
Yan Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Bone Defect ◽  
Atp Production ◽  
Bone Defect Repair ◽  
Mitochondria Transfer ◽  
Defect Repair

Abstract Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is considered a promising therapeutic approach for bone defect repair. However, during the transplantation procedure, the functions and viability of BMSCs may be impaired due to extended durations of in vitro culture, aging and disease conditions of patients. Inspired by spontaneous intercellular mitochondria transfer that naturally occurs within injured tissues to rescue cellular or tissue function, we investigated whether artificial mitochondria transfer into pre-transplant BMSCs in vitro could improve cellular function and enhance their therapeutic effects on bone defect repair in situ. Methods: First, mitochondria were isolated from donor BMSCs and transferred into recipient BMSCs of the same passage. Afterwards, changes in proliferative capability was evaluated by Cell Counting Kit-8, Ki67 staining, etc., while Transwell, wound scratch healing and cell motility tests were conducted to determine migration ability. Then, alkaline phosphatase (ALP) staining, Alizarin Red staining, combined with qPCR and Western Blot experiments of Runx2 and BMP2 were performed to elucidate the effect of mitochondria transfer on the osteogenic potential of BMSCs in vitro. After that, the in vivo experiments were completed by transplanting mitochondria-recipient BMSCs into a rat cranial critical-size bone defect model. Micro CT scanning and histological analysis were conducted 4 weeks and 8 weeks after transplantation to evaluate the osteogenesis effect in situ. Finally, in order to discover the potential connection between cellular behavioral changes and aerobic metabolism, OXPHOS (oxidative phosphorylation) and ATP production were assessed and inhibition of aerobic respiration by oligomycin was proceeded. Results: Mitochondria-recipient BMSCs exhibited significantly enhanced proliferation and migration, and increased osteogenic differentiation upon osteogenic induction. The in vivo results showed more new bone formation after transplantation of mitochondria-recipient BMSCs in situ. Increased OXPHOS activity and ATP production were further observed, whereas the inhibition of which impaired the enhancement of proliferation, migration and osteogenic differentiation induced by mitochondria transfer. Conclusions: Mitochondria transfer is a feasible technique to enhance BMSCs function in vitro and promote bone defect repair in situ through the up-regulation of aerobic metabolism. The results indicated that mitochondria transfer may be a novel promising technique for optimizing stem cell function.

scholarly journals Mitochondria transfer enhances proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cell and promotes bone defect healing

2020 ◽  
Author(s):  
Yusi Guo ◽  
Xiaopei Chi ◽  
Yifan Wang ◽  
Boon Chin Heng ◽  
Yan Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Bone Defect ◽  
Atp Production ◽  
Bone Defect Repair ◽  
Mitochondria Transfer ◽  
Defect Repair

Abstract Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is considered a promising therapeutic approach for bone defect repair. However, during the transplantation procedure, the functions and viability of BMSCs may be impaired due to extended durations of in vitro culture, aging and disease conditions of patients. Inspired by spontaneous intercellular mitochondria transfer that naturally occurs within injured tissues to rescue cellular or tissue function, we investigated whether artificial mitochondria transfer into pre-transplant BMSCs in vitro could improve cellular function and enhance their therapeutic effects on bone defect repair in situ . Methods: Mitochondria were isolated from donor BMSCs and transferred into recipient BMSCs of the same batch and passage. Subsequently, changes in proliferative capacity and cell senescence were evaluated by live cell imaging, Cell Counting Kit-8 assay, cell cycle analysis, Ki67 staining, qPCR and Western Blot analysis of C-myc expression, and β-galactosidase staining. Migration ability was evaluated by the transwell migration assay, wound scratch healing and cell motility tests. Alakine phosphatase (ALP) staining, Alizarin Red staining, combined qPCR and Western Blot analyses of Runx2 and BMP2 were performed to elucidate the effects of mitochondria transfer on the osteogenic potential of BMSCs in vitro . After that, in vivo experiments were performed by transplanting mitochondria-recipient BMSCs into a rat cranial critical-size bone defect model. Micro CT scanning and histological analysis were conducted at 4 and 8 weeks after transplantation to evaluate osteogenesis in situ . Finally, in order to establish the correlation between cellular behavioral changes and aerobic metabolism, OXPHOS (oxidative phosphorylation) and ATP production were assessed and inhibition of aerobic respiration by oligomycin was performed. Results: Mitochondria-recipient BMSCs exhibited significantly enhanced proliferation and migration, and increased osteogenesis upon osteogenic induction. The in vivo results showed more new bone formation after transplantation of mitochondria-recipient BMSCs in situ . Increased OXPHOS activity and ATP production were observed, which upon inhibition by oligomycin attenuated the enhancement of proliferation, migration and osteogenic differentiation induced by mitochondria transfer. Conclusions: Mitochondria transfer is a feasible technique to enhance BMSCs function in vitro and promote bone defect repair in situ through the up-regulation of aerobic metabolism. The results indicated that mitochondria transfer may be a novel promising technique for optimizing stem cell therapeutic function.

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