scholarly journals Apoptosis and oxidative stress as relevant mechanisms of antitumor activity and genotoxicity of ZnO-NPs alone and in combination with N-acetyl cysteine in tumor-bearing mice

2019 ◽  
Vol Volume 14 ◽  
pp. 3911-3928 ◽  
Author(s):  
Haidan M. El-Shorbagy ◽  
Shaymaa M. Eissa ◽  
Salwa Sabet ◽  
Akmal A. El-Ghor
Keyword(s):  
Oxidative Stress ◽  
Antitumor Activity ◽  
Zno Nps ◽  
Tumor Bearing ◽  
Acetyl Cysteine ◽  
And Oxidative Stress

scholarly journals Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tao Zhang ◽  
Cheng Ni ◽  
Cheng Li ◽  
Pan Lu ◽  
Dan Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Germ Cell ◽  
Fluorescence Imaging ◽  
Cell Apoptosis ◽  
Germ Cell Apoptosis ◽  
C Elegans ◽  
Interaction Studies ◽  
Acetyl Cysteine ◽  
Induced Changes ◽  
And Oxidative Stress

AbstractAnesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.

A Pilot Study to Evaluate the Effects of Oral N-Acetyl Cysteine on Inflammatory and Oxidative Stress Biomarkers in Rheumatoid Arthritis

2019 ◽  
Vol 15 (3) ◽  
pp. 246-253 ◽  
Author(s):  
Ghazal Hashemi ◽  
Mahtabalsadat Mirjalili ◽  
Zahra Basiri ◽  
Ahmad Tahamoli-Roudsari ◽  
Nejat Kheiripour ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Placebo Group ◽  
Inflammatory Cytokines ◽  
Serum Levels ◽  
Stress Factors ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
Acetyl Cysteine ◽  
And Oxidative Stress

<P>Background: Rheumatoid Arthritis (RA) is a common inflammatory disease of the joints. Due to the importance of inflammation and oxidative stress in the pathogenesis of RA, drugs that have anti-oxidant and anti-inflammatory properties, such as N-acetyl Cysteine (NAC), can be used as adjunctive therapy in patients with RA. </P><P> Aims: The aim of this study was to evaluate the effects of oral NAC on inflammatory cytokines and oxidative stress in patients with RA. </P><P> Methods: Adjunct to standard treatment, the NAC group (23 patients) received 600 mg of NAC twice daily and the placebo group (19 patients) received identical placebo twice daily for 12 weeks. Serum levels of Total Oxidant Status (TOS), Total Antioxidant Capacity (TAC), nitric oxide (NO), Total Thiol Groups (TTG), Malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-&#945;), interleukin- 6 (IL-6), C-reactive Protein (CRP), and Erythrocyte Sedimentation Rate (ESR) were measured at baseline and at the end of the study. </P><P> Results: Results showed that in the NAC group, the serum levels of MDA, NO, IL-6, TNF-&#945;, ESR and CRP were significantly lower than the baseline. Also, the serum level of TAC and TTG, as antioxidant parameters, increased significantly. However, only NO, MDA and TTG showed a significant difference in the NAC group as compared to the placebo group at the end of study. </P><P> Conclusion: According to the results of this study, oral NAC can significantly reduce the several oxidative stress factors and inflammatory cytokines. These results need to be confirmed in larger studies while considering clinical outcomes of RA patients.</P>

Evaluation of N-acetyl-cysteine against tetrachlorobenzoquinone-induced genotoxicity and oxidative stress in HepG2 cells

2014 ◽  
Vol 64 ◽  
pp. 291-297 ◽  
Author(s):  
Hui Dong ◽  
Demei Xu ◽  
Lihua Hu ◽  
Lingrui Li ◽  
Erqun Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Acetyl Cysteine ◽  
And Oxidative Stress

scholarly journals N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM

2020 ◽  
Vol 21 (19) ◽  
pp. 7318
Author(s):  
Gili Kaufman ◽  
Drago Skrtic
Keyword(s):  
Oxidative Stress ◽  
Stress Responses ◽  
Dental Pulp ◽  
Tissue Necrosis ◽  
Oxidative Balance ◽  
Maturation Stages ◽  
Acetyl Cysteine ◽  
Oxidative Stress Responses ◽  
Triethyleneglycol Dimethacrylate ◽  
And Oxidative Stress

Dental pulp is exposed to resin monomers leaching from capping materials. Toxic doses of the monomer, triethyleneglycol dimethacrylate (TEGDMA), impact cell growth, enhance inflammatory and oxidative stress responses, and lead to tissue necrosis. A therapeutic agent is required to rescue growth-arrested tissues by continuing their development and modulating the exacerbated responses. The functionality of N-Acetyl Cysteine (NAC) as a treatment was assessed by employing a 3D dental pulp microtissue platform. Immortalized and primary microtissues developed and matured in the extracellular matrix (ECM). TEGDMA was introduced at various concentrations. NAC was administered simultaneously with TEGDMA, before or after monomer addition during the development and after the maturation stages of the microtissue. Spatial growth was validated by confocal microscopy and image processing. Levels of inflammatory (COX2, NLRP3, IL-8) and oxidative stress (GSH, Nrf2) markers were quantified by immunoassays. NAC treatments, in parallel with TEGDMA challenge or post-challenge, resumed the growth of the underdeveloped microtissues and protected mature microtissues from deterioration. Growth recovery correlated with the alleviation of both responses by decreasing significantly the intracellular and extracellular levels of the markers. Our 3D/ECM-based dental pulp platform is an efficient tool for drug rescue screening. NAC supports compromised microtissues development, and immunomodulates and maintains the oxidative balance.

scholarly journals Distribution of Selenium and Oxidative Stress in Breast Tumor-Bearing Mice

Nutrients ◽  
2013 ◽  
Vol 5 (2) ◽  
pp. 594-607 ◽  
Author(s):  
Chih-Hung Guo ◽  
Simon Hsia ◽  
Pei-Chung Chen
Keyword(s):  
Oxidative Stress ◽  
Breast Tumor ◽  
Tumor Bearing ◽  
And Oxidative Stress

Association between calcitriol and paricalcitol with oxidative stress in patients with hemodialysis

2020 ◽  
pp. 1-8
Author(s):  
Hasan Haci Yeter ◽  
Berfu Korucu ◽  
Elif Burcu Bali ◽  
Ulver Derici
Keyword(s):  
Oxidative Stress ◽  
Vitamin D ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Total Oxidant Status ◽  
Vitamin D Analog ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

Abstract. Background: The pathophysiological basis of chronic kidney disease and its complications, including cardiovascular disease, are associated with chronic inflammation and oxidative stress. We investigated the effects of active vitamin D (calcitriol) and synthetic vitamin D analog (paricalcitol) on oxidative stress in hemodialysis patients. Methods: This cross-sectional study was composed of 83 patients with a minimum hemodialysis vintage of one year. Patients with a history of any infection, malignancy, and chronic inflammatory disease were excluded. Oxidative markers (total oxidant and antioxidant status) and inflammation markers (C-reactive protein and interleukin-6) were analyzed. Results: A total of 47% (39/83) patients were using active or analog vitamin D. Total antioxidant status was significantly higher in patients with using active or analog vitamin D than those who did not use (p = 0.006). Whereas, total oxidant status and oxidative stress index were significantly higher in patients with not using vitamin D when compared with the patients who were using vitamin D preparation (p = 0.005 and p = 0.004, respectively). On the other hand, total antioxidant status, total oxidant status, and oxidative stress index were similar between patients who used active vitamin D or vitamin D analog (p = 0.6; p = 0.4 and p = 0.7, respectively). Conclusion: The use of active or selective vitamin D analog in these patients decreases total oxidant status and increases total antioxidant status. Also, paricalcitol is as effective as calcitriol in decreasing total oxidant status and increasing total antioxidant status in patients with chronic kidney disease.

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