scholarly journals Long noncoding RNA expression analysis reveals the regulatory effects of nitinol-based nanotubular coatings on human coronary artery endothelial cells

2019 ◽  
Vol Volume 14 ◽  
pp. 3297-3309 ◽  
Author(s):  
Pan Shang ◽  
Gan Chen ◽  
Guannan Zu ◽  
Xiang Song ◽  
Peng Jiao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Coronary Artery ◽  
Expression Analysis ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Rna Expression ◽  
Human Coronary Artery ◽  
Regulatory Effects

scholarly journals Temporal Gene Expression Analysis of Human Coronary Artery Endothelial Cells Treated With Simvastatin

2008 ◽  
Vol 14 (4) ◽  
pp. 229-239 ◽  
Author(s):  
LI QIN ZHANG ◽  
SHWU-FAN MA ◽  
DMITRY GRIGORYEV ◽  
TERA L. LAVOIE ◽  
HUI QING XIAO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Coronary Artery ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Human Coronary Artery ◽  
Temporal Gene Expression

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

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