scholarly journals Plasma protein adsorption on Fe3O4-PEG nanoparticles activates the complement system and induces an inflammatory response

2019 ◽  
Vol Volume 14 ◽  
pp. 2055-2067 ◽  
Author(s):  
V. Escamilla-Rivera ◽  
A. Solorio-Rodriguez ◽  
M. Uribe-Ramirez ◽  
O. Lozano ◽  
S. Lucas ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Protein Adsorption ◽  
Plasma Protein ◽  
Complement System ◽  
The Complement System ◽  
Plasma Protein Adsorption

Plasma protein adsorption and biological identity of systemically administered nanoparticles

Nanomedicine ◽  
2017 ◽  
Vol 12 (17) ◽  
pp. 2113-2135 ◽  
Author(s):  
Dongyu Chen ◽  
Shanthi Ganesh ◽  
Weimin Wang ◽  
Mansoor Amiji
Keyword(s):  
Protein Adsorption ◽  
Plasma Protein ◽  
Plasma Protein Adsorption

Human plasma protein adsorption onto alumina nanoparticles relevant to orthopedic wear

2015 ◽  
Vol 13 (2) ◽  
pp. 0-0 ◽  
Author(s):  
John P. Gibbons ◽  
Marco P. Monopoli ◽  
Martin Lundqvist ◽  
Iker Montes-Burgos ◽  
Eilis E. McGrath ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Human Plasma ◽  
Plasma Protein ◽  
Alumina Nanoparticles ◽  
Human Plasma Protein ◽  
Plasma Protein Adsorption

scholarly journals Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jing Guo ◽  
Min Li ◽  
Yi Yang ◽  
Lin Zhang ◽  
Li-wei Zhang ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Inflammatory Response ◽  
Protein Content ◽  
Complement System ◽  
Complement Activation ◽  
Lung Inflammation ◽  
Acute Lung Inflammation ◽  
Tnf Α ◽  
The Complement System

Abstract Background The complement system plays a critical role as the pathogenic factor in the models of acute lung injury due to various causes. Cobra venom factor (CVF) is a commonly used complement research tool. The CVF can cause acute inflammation in the lung by producing complement activation components. Atorvastatin (ATR) is a 3-hydroxy-3-methylglutaryl coenzyme A inhibitor approved for control of plasma cholesterol levels. This inhibitor can reduce the acute pulmonary inflammatory response. However, the ability of ATR in treating acute lung inflammation caused by complement activation is still unknown. Therefore, we investigated the effect of ATR on lung inflammation in mice induced by activation of the complement alternative pathway in this study. Methods ATR (10 mg/kg/day via oral gavage) was administered for 7 days before tail vein injection of CVF (25 μg/kg). On the seventh day, all mice were sacrificed 1 h after injection. The lung lobe, bronchoalveolar lavage fluid (BALF), and blood samples were collected. The myeloperoxidase (MPO) activity of the lung homogenate, the leukocyte cell count, and the protein content of BALF were measured. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), P-selectin, and Intercellular cell adhesion molecule-1 (ICAM-1) in BALF and serum were determined by enzyme-linked immunosorbent assay. The pathological change of the lung tissue was observed by hematoxylin and eosin staining. The deposition of C5b-9 in the lung tissue was detected by immunohistochemistry. The phosphorylation of NF-κB p65 in the lung tissues was examined by immunohistochemistry and western blotting. Results The lung inflammation levels were determined by measuring the leukocyte cell numbers and protein content of BALF, the lung MPO activity, and expression and staining of the inflammatory mediators (IL-6 and TNF-α), and adhesion molecules (P-selectin and ICAM-1) for lung lesion. A significant reduction in the lung inflammation levels was observed after 7 days in ATR pre-treated mice with a CVF-induced lung disease. Deposition of C5b-9 was significantly alleviated by ATR pretreatment. Early intervention with ATR significantly reduced the development of acute lung inflammation on the basis of phosphorylation of NF-κB p65 in the lung. Conclusion These findings suggest the identification of ATR treatment for the lung inflammation induced by activating the complement system on the basis of its anti-inflammatory response. Together with the model replicating the complement activating characteristics of acute lung injury, the results may be translatable to the overactivated complement relevant diseases.

Probing the mechanism of plasma protein adsorption on Au and Ag nanoparticles with FT-IR spectroscopy

Nanoscale ◽  
2015 ◽  
Vol 7 (37) ◽  
pp. 15191-15196 ◽  
Author(s):  
Mengmeng Wang ◽  
Cuiping Fu ◽  
Xingang Liu ◽  
Zhipeng Lin ◽  
Ning Yang ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Protein Adsorption ◽  
Plasma Protein ◽  
Ag Nanoparticles ◽  
Dynamic Changes ◽  
P Protein ◽  
Ft Ir ◽  
Plasma Protein Adsorption ◽  
Ft Ir Spectroscopy

Protein structural and dynamic changes mainly depend on the interaction between sulphur and Au when proteins are absorbed on AuNPs.

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