scholarly journals Antibacterial activity and mechanism of silver nanoparticles against multidrug-resistant Pseudomonas aeruginosa

2019 ◽  
Vol Volume 14 ◽  
pp. 1469-1487 ◽  
Author(s):  
Shijing Liao ◽  
Yapeng Zhang ◽  
Xuanhe Pan ◽  
Feizhou Zhu ◽  
Congyuan Jiang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Multidrug Resistant

Conjugation of imipenem to silver nanoparticles for enhancement of its antibacterial activity against multidrug-resistant isolates of Pseudomonas aeruginosa

2021 ◽  
Vol 46 (1) ◽  
Author(s):  
Mahsa Shahbandeh ◽  
Anoosh Eghdami ◽  
Mehrdad Moosazadeh Moghaddam ◽  
Mohammadreza Jalali Nadoushan ◽  
Ali Salimi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Multidrug Resistant

scholarly journals A Novel Cecropin D-Derived Short Cationic Antimicrobial Peptide Exhibits Antibacterial Activity Against Wild-Type and Multidrug-Resistant Strains of Klebsiella pneumoniae and Pseudomonas aeruginosa

2020 ◽  
Vol 16 ◽  
pp. 117693432093626
Author(s):  
Iván Darío Ocampo-Ibáñez ◽  
Yamil Liscano ◽  
Sandra Patricia Rivera-Sánchez ◽  
José Oñate-Garzón ◽  
Ashley Dayan Lugo-Guevara ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Health Concern ◽  
Wild Type ◽  
Cationic Antimicrobial Peptide ◽  
Promising Alternative ◽  
Resistant Strains

Infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa and Klebsiella pneumoniae are a serious worldwide public health concern due to the ineffectiveness of empirical antibiotic therapy. Therefore, research and the development of new antibiotic alternatives are urgently needed to control these bacteria. The use of cationic antimicrobial peptides (CAMPs) is a promising candidate alternative therapeutic strategy to antibiotics because they exhibit antibacterial activity against both antibiotic susceptible and MDR strains. In this study, we aimed to investigate the in vitro antibacterial effect of a short synthetic CAMP derived from the ΔM2 analog of Cec D-like (CAMP-CecD) against clinical isolates of K pneumoniae (n = 30) and P aeruginosa (n = 30), as well as its hemolytic activity. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of CAMP-CecD against wild-type and MDR strains were determined by the broth microdilution test. In addition, an in silico molecular dynamic simulation was performed to predict the interaction between CAMP-CecD and membrane models of K pneumoniae and P aeruginosa. The results revealed a bactericidal effect of CAMP-CecD against both wild-type and resistant strains, but MDR P aeruginosa showed higher susceptibility to this peptide with MIC values between 32 and >256 μg/mL. CAMP-CecD showed higher stability in the P aeruginosa membrane model compared with the K pneumoniae model due to the greater number of noncovalent interactions with phospholipid 1-Palmitoyl-2-oleyl-sn-glycero-3-(phospho-rac-(1-glycerol)) (POPG). This may be related to the boosted effectiveness of the peptide against P aeruginosa clinical isolates. Given the antibacterial activity of CAMP-CecD against wild-type and MDR clinical isolates of P aeruginosa and K pneumoniae and its nonhemolytic effects on human erythrocytes, CAMP-CecD may be a promising alternative to conventional antibiotics.

Antibacterial Activity of Wild Xylaria sp. Strain R005 (Ascomycetes) Against Multidrug-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2012 ◽  
Vol 14 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Veluchamy Ramesh ◽  
U. S. Ezhil Arivudainambi ◽  
Annamalai Thalavaipandian ◽  
Chandran Karunakaran ◽  
Ayyappan Rajendran
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Multidrug Resistant

scholarly journals ANTIBACTERIAL ACTIVITY OF THREE PLANT EXTRACTS AGAINST MULTIDRUG RESISTANCE PSEUDOMONAS AERUGINOSA

2017 ◽  
Vol 10 (12) ◽  
pp. 193 ◽  
Author(s):  
Yassir Haider Almawlah ◽  
Hadeel Alaa ◽  
Sanaa O Aljelawi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Multidrug Resistance ◽  
Plant Extracts ◽  
Punica Granatum ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Active Components ◽  
Burn Wound ◽  
Test Organisms

Objective: The increasing of antibiotic resistance of Pseudomonas aeruginosa causes serious worldwide infection problems. Hence, the objective of the study was to investigate the antibacterial activity of some plants against multidrug resistance P. aeruginosa isolated from burn-wound infections.Methods: About 30 burn-wound swab samples taken from hospitalized patients in Al-Hillah Teaching Hospital, Babylon Province. P. aeruginosa isolates were isolated and identified depending on cultural, microbial, and biochemical characteristics. Then, the drug susceptibility was detected using different available antibiotics (8) to select multidrug resistance P. aeruginosa strains for using as test organisms. Three types of plants, including onion bulbs (Allium cepa), leaves of mint (Mentha asiatica), and outer peel of pomegranate (Punica granatum), were extracted by three types of solvent. The plant extracts were tested as antimicrobial substances.Results: About 9 samples were found positive by causing bacterial infection who presented invasive burn-wound infection from both sex and average age of 9 to 45 years. P. aeruginosa was found to be the most common isolates, 10 samples out of 30 samples. The most of multidrug resistance P. aeruginosa was used as test organisms to investigate the antimicrobial activity of three types of plant extracts. The plant extract of P. granatum showed the highest antibacterial activity, followed by A. cepa, and finally, M. asiatica.Conclusion: From the study, all three studied plants had antibacterial activity against multidrug-resistant P. aeruginosa isolated from burn wound. It is a recommendation that natural products can use as therapeutic agents will probably not elicit resistance in bacteria. More research must continue to isolate and purify the active components and applied in experimental animal models. 

scholarly journals Trp-Containing Antibacterial Peptides Impair Quorum Sensing and Biofilm Development in Multidrug-Resistant Pseudomonas aeruginosa and Exhibit Synergistic Effects With Antibiotics

2021 ◽  
Vol 12 ◽  
Author(s):  
Dejing Shang ◽  
Xue Han ◽  
Wanying Du ◽  
Zhiru Kou ◽  
Fengquan Jiang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Efflux Pump ◽  
Synergistic Effects ◽  
Multidrug Resistant ◽  
Biofilm Development ◽  
Antibiotic Efflux

Pseudomonas aeruginosa uses quorum sensing (QS) to control virulence, biofilm formation and antibiotic efflux pump expression. The development of effective small molecules targeting the QS system and biofilm formation represents a novel attractive strategy. In this present study, the effects of a series of Trp-containing peptides on the QS-regulated virulence and biofilm development of multidrug-resistant P. aeruginosa, as well as their synergistic antibacterial activity with three classes of traditional chemical antibiotics were investigated. The results showed that Trp-containing peptides at low concentrations reduced the production of QS-regulated virulence factors by downregulating the gene expression of both the las and rhl systems in the strain MRPA0108. Biofilm formation was inhibited in a concentration-dependent manner, which was associated with extracellular polysaccharide production inhibition by downregulating pelA, algD, and pslA transcription. These changes correlated with alterations in the extracellular production of pseudomonal virulence factors and swarming motility. In addition, the combination of Trp-containing peptides at low concentration with the antibiotics ceftazidime and piperacillin provided synergistic effects. Notably, L11W and L12W showed the highest synergy with ceftazidime and piperacillin. A mechanistic study demonstrated that the Trp-containing peptides, especially L12W, significantly decreased β-lactamase activity and expression of efflux pump genes OprM, MexX, and MexA, resulting in a reduction in antibiotic efflux from MRPA0108 cells and thus increasing the antibacterial activity of these antibiotics against MRPA0108.

scholarly journals Antibacterial ativity of biosynthesized silver nanoparticles against Pseudomonas aeruginosa in vitro

2017 ◽  
Vol 41 (1) ◽  
pp. 60-65
Author(s):  
Ahmad N. A. Salih ◽  
Mohammad J. Eesa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Sulfadiazine ◽  
Diffusion Method ◽  
Olive Leaves ◽  
Scanning Electron

     This study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-friendly and easy process and UV-Visible absorption spectra of the silver nanoparticles revealed maximum absorbance at 420 and 430 nm. The Scanning Electron Microscopy analysis demonstrated the mean of the silver particles diameter was 26 nm. The antibacterial findings of the synthesized silver nanoparticles against Pseudomonas aeruginosa in vitro showed that the silver nanoparticles were more effective than silver sulfadiazine against Pseudomonas aeruginosa. It could be concluded that olive leaves extract can be used effectively in the production of silver nanoparticles and these synthesized nanoparticles had considerable antibacterial activity against Pseudomonas aeruginosa in vitro.                                                        

scholarly journals Effect of Storage Period on Silver Nanoparticles Biosynthesized By Pseudomonas Aeroginosa

2019 ◽  
Vol 9 (04) ◽  
pp. 678-681
Author(s):  
Ashraf S Hassan ◽  
Khawlah J Khalaf ◽  
Hamzia A Ajah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Storage Period ◽  
Before And After ◽  
Pathogenic Microbes

The present study demonstrates the effect of storage period on silver nanoparticles (AgNPs), which synthesized by Pseudomonas aeruginosa and their antibacterial activity. The result shows that the size of (AgNPs) which synthesis by Pseudomonas aeruginosa was 93.55nm after 4-72hour, and when storage about 2 years, we found that the size of AgNPs was stable and reduced to 69.0nm. Antibacterial activity against pathogenic microbes: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia sp,Streptococcus sp , Klebsiella sp, Candida albicans was performed before and after storage and found that AgNPs have activity against this microbes.

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