scholarly journals Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

2018 ◽  
Vol Volume 13 ◽  
pp. 4073-4082 ◽  
Author(s):  
Hongbo Tang ◽  
Ziwen Jiang ◽  
Haibo He ◽  
Xiaoqin Li ◽  
Haipeng Hu ◽  
...  
Keyword(s):  
Cell Model ◽  
Placental Barrier ◽  
Bewo B30 ◽  
Pullulan Acetate ◽  
Uptake And Transport

Effect of Green Tea and (-)-Epigallocatechin Gallate on the Pharmacokinetics of Rosuvastatin

2020 ◽  
Vol 21 (6) ◽  
pp. 471-478
Author(s):  
Shenjia Huang ◽  
Qingqing Xu ◽  
Linsheng Liu ◽  
Yicong Bian ◽  
Shichao Zhang ◽  
...  
Keyword(s):  
Green Tea ◽  
Cell Model ◽  
Hek293t Cell ◽  
Epigallocatechin Gallate ◽  
Drug Uptake ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Time Curve ◽  
Uptake And Transport

Background: Green tea can inhibit OATPs, so it may interact with the substrate of OATPs, such as rosuvastatin. Objective: This study aimed to investigate the effects of green tea on the pharmacokinetics of rosuvastatin and its mechanism. Methods: Male Sprague-Dawley rats received different doses of green tea extract (GTE) and (-)- epigallocatechin-3- gallate (EGCG). Caco-2 cells and OATP1B1-HEK293T cells were used in drug uptake and transport assay. The matrix concentrations of rosuvastatin and catechins were determined by ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS). Results: GTE and EGCG were both found to increase the area under the plasma concentration-time curve (AUC0-∞) of rosuvastatin ((p<0.050). In the Caco-2 cell model, the uptake and transport of rosuvastatin in the GTE groups were 1.94-fold (p<0.001) and 2.11-fold (p<0.050) higher, respectively, than those of the control group. However, in the EGCG group, the uptake and transport of rosuvastatin were decreased by 22.62% and 44.19%, respectively (p<0.050). In the OATP1B1- HEK293T cell model, the OATP1B1-mediated rosuvastatin uptake was decreased by GTE to 35.02% of that in the control (p<0.050) and was decreased by EGCG to 45.61% of that in the control (p<0.050). Conclusion: GTE increased the systemic rosuvastatin exposure in rats. The mechanism may include an increase in rosuvastatin absorption and a decrease in liver distribution by inhibiting OATP1B1. EGCG may be the main ingredient of green tea that affects the pharmacokinetic parameters of rosuvastatin. Our results showed the importance of conducting green tea-rosuvastatin study.

Transport and toxicity of 5-fluorouracil, doxorubicin, and cyclophosphamide in in vitro placental barrier model based on BeWo b30 cells

2019 ◽  
Vol 68 (12) ◽  
pp. 2344-2349
Author(s):  
E. N. Knyazev ◽  
S. V. Nikulin ◽  
A. Yu. Khristichenko ◽  
T. N. Gerasimenko ◽  
O. V. Kindeeva ◽  
...  
Keyword(s):  
Placental Barrier ◽  
Model Based ◽  
Bewo B30 ◽  
Barrier Model

scholarly journals Medicinal Plants for the Treatment of Mental Diseases in Pregnancy: An In Vitro Safety Assessment

Planta Medica ◽  
2021 ◽  
Author(s):  
Deborah Spiess ◽  
Moritz Winker ◽  
Antoine Chauveau ◽  
Vanessa Fabienne Abegg ◽  
Olivier Potterat ◽  
...  
Keyword(s):  
Cell Model ◽  
Lactate Production ◽  
Well Being ◽  
Placental Cell ◽  
California Poppy ◽  
Altered Glucose ◽  
Mental Diseases ◽  
Bewo B30 ◽  
In Pregnancy

AbstractPregnancy is a critical period for medical care, during which the well-being of woman and fetus must be considered. This is particularly relevant in managing non-psychotic mental disorders since treatment with central nervous system-active drugs and untreated NMDs may have negative effects. Some well-known herbal preparations (phytopharmaceuticals), including St. Johnʼs wort, California poppy, valerian, lavender, and hops, possess antidepressant, sedative, anxiolytic, or antidepressant properties and could be used to treat mental diseases such as depression, restlessness, and anxiety in pregnancy. Our goal was to assess their safety in vitro, focusing on cytotoxicity, induction of apoptosis, genotoxicity, and effects on metabolic properties and differentiation in cells widely used as a placental cell model (BeWo b30 placenta choriocarcinoma cells). The lavender essential oil was inconspicuous in all experiments and showed no detrimental effects. At low-to-high concentrations, no extract markedly affected the chosen safety parameters. At an artificially high concentration of 100 µg/mL, extracts from St. Johnʼs wort, California poppy, valerian, and hops had minimal cytotoxic effects. None of the extracts resulted in genotoxic effects or altered glucose consumption or lactate production, nor did they induce or inhibit BeWo b30 cell differentiation. This study suggests that all tested preparations from St. Johnʼs wort, California poppy, valerian, lavender, and hops, in concentrations up to 30 µg/mL, do not possess any cytotoxic or genotoxic potential and do not compromise placental cell viability, metabolic activity, and differentiation. Empirical and clinical studies during pregnancy are needed to support these in vitro data.

Laminin 521 Alters the SNAI1, ZNF708 and GRN Gene Expression in BeWo b30 Cells and Creates Physiological Conditions for the Placental Barrier

2019 ◽  
Vol 35 (5) ◽  
pp. 87-93
Author(s):  
E.N. Knyazev ◽  
T.N. Gerasimenko ◽  
O.V. Kindeeva ◽  
D.V. Maltseva ◽  
A. Turchinovich ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Monolayer ◽  
Snorna Gene ◽  
Placental Barrier ◽  
Ministry Of Education ◽  
Multilayered Structures ◽  
Russian Scientific ◽  
First Case ◽  
Bewo B30 ◽  
Microrna Genes

A human choriocarcinoma cell line BeWo b30 imitates cytotrophoblast cells and thus allows to model the placental barrier. Since these cells can form multilayered structures, a method to control the number of cells based on the MTS test has been developed. It was found that during the cell growth on a laminin-521-containing extracellular matrix a significant change in the transepithelial electrical resistance (TEER) and the electrical capacitance of the cell monolayer is observed as compared to the cultivation without the extracellular matrix. The transcriptome analysis of the cells revealed that in the presence of laminin-521, the expression of the ZNF708 gene decreases and that of the SNAI1 and progranuline gene GRN increases as compared to the data obtained without the extracellular matrix. This may indicate that in the first case the cells were prepared to fuse into syncytiotrophoblast. A change in the expression of five microRNA genes and one snoRNA gene was also observed. The above mentioned effects can be associated with the used laminin. BeWo b30, placenta, barrier, microRNA, laminin 521 (laminin-11), SNAI1 The study was supported by the Ministry of Education and Science of the Russian Federation in the framework of the Federal Targeted Program for Research and Development in Priority Areas of Advancement of the Russian Scientific and Technological Complex for 2014-2020 (RFMEFI58817X0007).

scholarly journals Arsenic accumulation, speciation and bioavailability in rice cultivated in arsanilic acid exposed soil

2021 ◽  
Vol 67 (No. 5) ◽  
pp. 307-316
Author(s):  
Wanling He ◽  
Xiaoli Li ◽  
Shu Guo ◽  
Longbang Yang ◽  
Dong Li
Keyword(s):  
Cell Model ◽  
Dry Weight ◽  
In Vitro Digestion ◽  
Dimethylarsinic Acid ◽  
Arsenic Accumulation ◽  
Arsanilic Acid ◽  
Monomethylarsonic Acid ◽  
As Species ◽  
Uptake And Transport

The present study used various amounts of P-arsanilic acid (AsA) in pot experiments to evaluate the effects of AsA on arsenic (As) accumulation, speciation and meanwhile using the in vitro digestion/Caco-2 cell model to evaluate the bioavailability of As in rice. The results indicated a linear relationship between As in rice and As in soil, and at 75 mg AsA/kg of soil, As content in rice exceeded the statutory permissible limit of 0.2 mg As/kg dry weight in China. Speciation studies indicated that inorganic As (Asi), dimethylarsinic acid (DMA), and monomethylarsonic acid (MMA) were the main As species in rice. Bioavailability of As experiment indicated that As uptake and transport amount by Caco-2 cells increased with increasing As accumulation in rice. In general, the content of AsA in soil reached or exceeded 75 mg/kg, which is not suitable for growing rice.  

scholarly journals Chemical form of selenium affects its uptake and transport in the human intestinal cell model, Caco‐2

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Huawei Zeng ◽  
Matthew I Jackson ◽  
Wen‐Hsing Cheng ◽  
Gerald F Combs
Keyword(s):  
Cell Model ◽  
Chemical Form ◽  
Intestinal Cell ◽  
Uptake And Transport

Bioaccessibility, Uptake, and Transport of Carotenoids from Peppers (Capsicum Spp.) Using the Coupled in Vitro Digestion and Human Intestinal Caco-2 Cell Model

2010 ◽  
Vol 58 (9) ◽  
pp. 5374-5379 ◽  
Author(s):  
Laurie O’Sullivan ◽  
Marvin A. Jiwan ◽  
Trevor Daly ◽  
Nora M. O’Brien ◽  
S. Aisling Aherne
Keyword(s):  
Cell Model ◽  
In Vitro Digestion ◽  
Capsicum Spp ◽  
Uptake And Transport

Use of SEM, X-ray analysis and TEM to localize Fe3+ reduction in roots

1988 ◽  
Vol 46 ◽  
pp. 392-393 ◽  
Author(s):  
William P. Wergin ◽  
P. F. Bell ◽  
Rufus L. Chaney
Keyword(s):  
Electron Microscopy ◽  
Root Hairs ◽  
Rapid Reduction ◽  
X Ray ◽  
Physical Evidence ◽  
Transmission Electron ◽  
Young Root ◽  
Insoluble Precipitate ◽  
Uptake And Transport ◽  
Scanning Electron

In dicotyledons, Fe3+ must be reduced to Fe2+ before uptake and transport of this essential macronutrient can occur. Ambler et al demonstrated that reduction along the root could be observed by the formation of a stain, Prussian blue (PB), Fe4 [Fe(CN)6]3 n H2O (where n = 14-16). This stain, which is an insoluble precipitate, forms at the reduction site when the nutrient solution contains Fe3+ and ferricyanide. In 1972, Chaney et al proposed a model which suggested that the Fe3+ reduction site occurred outside the cell membrane; however, no physical evidence to support the model was presented at that time. A more recent study using the PB stain indicates that rapid reduction of Fe3+ occurs in a region of the root containing young root hairs. Furthermore the most pronounced activity occurs in plants that are deficient in Fe. To more precisely localize the site of Fe3+ reduction, scanning electron microscopy (SEM), x-ray analysis, and transmission electron microscopy (TEM) were utilized to examine the distribution of the PB precipitate that was induced to form in roots.

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

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