scholarly journals Arginine, glycine, aspartic acid peptide-modified paclitaxel and curcumin co-loaded liposome for the treatment of lung cancer: in vitro/vivo evaluation

2018 ◽  
Vol Volume 13 ◽  
pp. 2561-2569 ◽  
Author(s):  
Kanqiu Jiang ◽  
Mingjing Shen ◽  
Weihua Xu
Keyword(s):  
Lung Cancer ◽  
Aspartic Acid ◽  
Arginine Glycine Aspartic Acid

Molybdenum Dioxide (MoS2)/Gadolinium (Gd) Containing Arginine-Glycine-Aspartic Acid (RGD) Sequences as New Nano-Contrast Agent for Cancer Magnetic Resonance Imaging (MRI)

2021 ◽  
Vol 21 (3) ◽  
pp. 1403-1412
Author(s):  
Xiaoguang Hao ◽  
Weijing Li
Keyword(s):  
Particle Size ◽  
Contrast Agent ◽  
Contrast Agents ◽  
Aspartic Acid ◽  
Nude Mice ◽  
Molybdenum Dioxide ◽  
Signal Value ◽  
Arginine Glycine Aspartic Acid

Molybdenum dioxide-gadolinium-arginine/glycine/aspartic acid (MoS2-Gd-RGD) sequences targeting nano-contrast agents that specifically bind to human hepatocellular carcinoma (HCC) HepG2 cells were synthesized, and their targeting imaging effects on HCC cells and models were evaluated. Zeta potential, particle size and Fourier Transform Infrared Spectrometer (FTIR) were used to characterize the nano-contrast agent, and its cytotoxicity was evaluated. The MoS2-Gd nanoparticles were used as control in vitro to determine the targeting capability of the MoS2-Gd-RGD nanoparticles toward integrin αvβ3. During in vivo animal experiments, 12 nude mice with tumors were randomly divided into three groups to compare the imaging effects of the MoS2-Gd-RGD and MoS2-Gd groups. The hydrodynamic diameter of MoS2-Gd-RGD nanoparticles was approximately 336.43±6.43 nm, and the polydispersity index (PDI) value reached 0.132. Transmission electron microscopy showed the uniform particle size and good dispersion of the nanoparticles. The relaxation rate totaled 1.39 mM−1S−1. The signal value of the T1-weighted image of the HepG2 cells treated with MoS2-Gd-RGD was higher than that of the non-targeted materials (MoS2-Gd) (P < 0.01). The signal value of the tumor increased significantly 15 min after the tail vein injection with MoS2-Gd-RGD, and it peaked at 60 min after injection. A significant difference in tumor signal values was observed between the two groups of nude mice injected with MoS2-Gd-RGD and MoS2- Gd (P < 0.01). At the in vitro and in vivo experiments, the MoS2-Gd-RGD nanoparticles presented the characteristics of integrin αvβ3 targeting. Thus, MoS2-Gd-RGD nanoparticles feature potential as contrast agents for MRI.

Polymer chains incorporating caprolactone and arginine–glycine–aspartic acid functionalities: Synthesis, characterization and biological response in vitro of the Schwann cell

2013 ◽  
Vol 28 (1) ◽  
pp. 50-65 ◽  
Author(s):  
Eduard Rodriguez Pérez ◽  
Dunia M. García Cruz ◽  
Maria C. Araque Monrós ◽  
U Gómez-Pinedo ◽  
Manuel Monleón Pradas ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Aspartic Acid ◽  
Biological Response ◽  
Polymer Chains ◽  
Arginine Glycine Aspartic Acid

Cell Function on Substrates Containing Immobilized Bioactive Peptides

1993 ◽  
Vol 331 ◽  
Author(s):  
Kay C Dee ◽  
Thomas T. Andersen ◽  
R. Bizios
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Aspartic Acid ◽  
Bioactive Peptides ◽  
Cell Function ◽  
Substrate Surface ◽  
Cellular Functions ◽  
Arginine Glycine Aspartic Acid ◽  
Glass Surfaces

AbstractAdhesion, proliferation and motility of bovine pulmonary artery endothelial cells and of rat calvarial osteoblasts were examined in vitro and on glass surfaces modified with immobilized bioactive peptides. The peptides Arginine-Glycine-Aspartic Acid-Serine (RGDS), Arginine-Aspartic Acid-Glycine-Serine (RDGS), and Tyrosine-Isoleucine-Glycine-Serine-Arginine-Glycine (YIGSRG) were covalently bound to aminophase glass. The results of this study showed that modification of the substrate surface with immobilized peptides affected each cell line in different ways. Incorporation of this knowledge in the design of implant materials could result in biomaterials which promote and/or sustain a number of desirable cellular functions at the tissue-implant interface.

Cyclic arginine-glycine-aspartic acid peptide inhibits macrophage infiltration of the kidney and carotid artery lesions in apo-E-deficient mice

2006 ◽  
Vol 290 (1) ◽  
pp. F159-F166 ◽  
Author(s):  
Saban Elitok ◽  
Sergey V. Brodsky ◽  
Daniel Patschan ◽  
Tatyana Orlova ◽  
Kenneth M. Lerea ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Aspartic Acid ◽  
Lipid Accumulation ◽  
Macrophage Infiltration ◽  
Artery Ligation ◽  
Apo E ◽  
Arginine Glycine Aspartic Acid ◽  
Deficient Mice

Interactions of leukocytes with the vascular endothelium culminating in their diapedesis represent not only a crucial event in immune surveillance and defense but are also critically involved in the pathogenesis of many inflammatory diseases, including atherosclerosis. Our previous in vitro studies using atomic force microscopy measurement of monocyte-endothelial cell interaction have demonstrated that a cyclic arginine-glycine-aspartic acid peptide (cRGD) inhibited their adhesion through very late antigen (α4β1-integrin; VLA4)-vascular cell adhesion molecule-1 by 60% with the IC50= 100 nM. To elucidate the potential efficacy of this peptide in vivo in preventing atherogenesis, experiments were performed in apolipoprotein E (ApoE)-deficient (−/−) mice fed a Western diet and receiving chronic treatment with cRGD peptide for 2–4 wk. In addition, some animals were subjected to a temporary carotid artery ligation while receiving the above treatment. Formation of fatty streaks and infiltration of the vascular wall with macrophages were not affected by cRGD treatment. Infiltration of the carotid artery postligation was significantly reduced in the cRGD-treated animals, as was the lipid accumulation. Furthermore, cRGD-treated ApoE−/−mice exhibited significantly lesser macrophage infiltration and lipid accumulation in the kidneys, the site of the highest expression of VLA4. These data demonstrated that cRGD peptide is a potent inhibitor of monocyte/macrophage infiltration of the injured macrovasculature and of the renal microvasculature, where it results in the attenuation of lipid accumulation. Formation of fatty streaks in the aortic root was not inhibitable by this treatment.

scholarly journals 271INFLUENCE OF ARGININE-GLYCINE-ASPARTIC ACID (RGD) IN BOVINE SPERM-EGG BINDING, AND FERTILIZATION IN VITRO

2004 ◽  
Vol 16 (2) ◽  
pp. 256 ◽  
Author(s):  
R.F. Gonçalves ◽  
D. Erickson ◽  
A.D. Ealy ◽  
G.J. Killian
Keyword(s):  
Aspartic Acid ◽  
Rgd Peptide ◽  
Fertilization In Vitro ◽  
Binding Assays ◽  
Rgd Peptides ◽  
Sperm Binding ◽  
Bovine Sperm ◽  
Arginine Glycine Aspartic Acid ◽  
Bovine Oocytes

Osteopontin (OPN), a secretory RGD-containing phosphoprotein, has been identified in cow oviductal ephitelium and fluid, but its role in fertilization is unclear. RGD peptide is capable of blocking fertilization, inducing intracellular Ca2+ transients, and initiating parthenogenetic development when present during bovine fertilization in vitro.This study was conducted to determine whether in vitro sperm binding to the zona pellucida (ZP) and fertilization of bovine oocytes were affected by treating the sperm or oocytes with RGD (arginine–glycine–aspartic acid, a sequence recognized by integrins) or non-RGD-containing peptides. In vitro matured oocytes were incubated (39°C, 5% CO2 in air) for 2 hours in fertilization medium with: (1) no peptides;; (2) 50μgmL−1 RGD (Calbiochem®, San Diego, CA, USA); (3) 1000μgmL−1; (4) 50μgmL−1 non-RGD (Calbiochem®); (5) 1000μgmL−1 non-RGD. The bovine sperm from two differents bulle was collected by artificial vagina, pooled, washed twice with MTM at 500g for 10min and incubated (39°C, 5% CO2 in air) for two h at 5×107 concentration in MTM with: (6) no peptides;; (7) 50μgmL−1 RGD;; (8) 1000μgmL−1; (9) 50μgmL−1 non-RGD; (10) 1000μgmL−1 non-RGD. Following incubation, treated and untreated oocytes were washed and inseminated with 1×105 treated or untreated fresh spermatozoa per 10 oocytes;; after the sperm were recovered from a Percoll gradient (45%/90%). After 18–20h, oocytes were removed from co-culture, and washed in TL-HEPES. Oocytes used to assess sperm binding were stained with Hoescht 33342, and the number of sperm bound per ZP counted. The remaining oocytes were fixed in acid alcohol, stained with 1% acetato-orcein and observed for the presence of pronuclei. For the five replicates, 100–120 oocytes were used for fertilization and 150–170 oocytes were used for sperm-egg binding assays. Data were analized by SAS. Treatment of sperm or oocytes with the RGD peptide significantly decreased (P&lt;0.05) fertilization compared to the non-treated controls or those treated with non-RGD peptides: (1) 80%±3.0; (2) 42%±3.0; (3) 30.2%±3.0; (4) 78.5%±3.0; (5) 79.1%±3.0; (6) 78.9%±3.0; (7) 41.3%±3.0; (8) 29.1%±3.0; (9) 79.2%±3.0; (10) 80.2%±3.0. More sperm bound to the ZP of untreated or non-RGD-treated oocytes or sperm than those incubated with the RGD peptide: (1) 71.2±4.1; (2) 33.2±4.2; (3) 24.2±4.1; (4) 69.5±4.1; (5) 70.2±4.2; (6) 71.9±4.2; (7) 29.8±4.2; (8) 19.8±4.2; (9) 68.9±4.2; (10) 70.6±4.2. These studies demonstrated that incubation of bovine oocytes or spermatozoa with a RGD peptide inhibits sperm-egg binding and fertilization in vitro. These findings support the notion that the role of osteopontin in bovine fertilization may involve inteaction with integrins via its RGD sequence.

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