scholarly journals In vivo evaluation of a novel nanocomposite porous 3D scaffold in a rabbit model: histological analysis

2017 ◽  
Vol Volume 12 ◽  
pp. 8587-8598 ◽  
Author(s):  
Saffanah Mahmood ◽  
Abdul Razak Intan-Shameha ◽  
Mustafa Ghaji ◽  
Loqman Mohamad Yusof ◽  
Zaid Mahmood ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Rabbit Model ◽  
3D Scaffold ◽  
In Vivo Evaluation

In Vivo Evaluation of The Novel Nanocomposite Porous 3D Scaffold in a Rabbit Model

2018 ◽  
Vol 11 (19) ◽  
pp. 1-15
Author(s):  
Saffanah Khuder Mahmood ◽  
Intan Shameha Binti Abdul Razak ◽  
Sahar Mohammed Ibrahim ◽  
Loqman Mohamed Yusof ◽  
Adamu Abdul Abubakar ◽  
...  
Keyword(s):  
Rabbit Model ◽  
The Novel ◽  
3D Scaffold ◽  
In Vivo Evaluation

scholarly journals In Vivo evaluation of the novel nanocomposite porous 3D scaffold in a rabbit model: hematology and biochemistry analysis

2019 ◽  
Vol 32 (2) ◽  
pp. 219-230
Author(s):  
S. Mahmood ◽  
Md. Zakaria ◽  
I. Abdul Razak ◽  
L. Yusof ◽  
A. Abubakar ◽  
...  
Keyword(s):  
Rabbit Model ◽  
The Novel ◽  
3D Scaffold ◽  
In Vivo Evaluation

scholarly journals Development of Triamcinolone Acetonide-Loaded Microemulsion as a Prospective Ophthalmic Delivery System for Treatment of Uveitis: In Vitro and In Vivo Evaluation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 444
Author(s):  
Alaa Mahran ◽  
Sayed Ismail ◽  
Ayat A. Allam
Keyword(s):  
Triamcinolone Acetonide ◽  
Delivery System ◽  
Histopathological Examination ◽  
Rabbit Model ◽  
Subconjunctival Injection ◽  
In Vivo Evaluation ◽  
Ternary Phase Diagrams ◽  
Ophthalmic Delivery

Treatment of uveitis (i.e., inflammation of the uvea) is challenging due to lack of convenient ophthalmic dosage forms. This work is aimed to determine the efficiency of triamcinolone acetonide (TA)-loaded microemulsion as an ophthalmic delivery system for the treatment of uveitis. Water titration method was used to construct different pseudo-ternary phase diagrams. Twelve microemulsion formulations were prepared using oleic acid, Cremophor EL, and propylene glycol. Among all tested formulations, Formulation F3, composed of oil: surfactant-co-surfactant (1:1): water (15:35:50% w/w, respectively), was found to be stable and showed acceptable pH, viscosity, conductivity, droplet size (211 ± 1.4 nm), and zeta potential (−25 ± 1.7 mV) and almost complete in vitro drug release within 24 h. The in vivo performance of the optimized formulation was evaluated in experimentally uveitis-induced rabbit model and compared with a commercial TA suspension (i.e., Kenacort®-A) either topically or by subconjunctival injection. Ocular inflammation was evaluated by clinical examination, white blood cell count, protein content measurement, and histopathological examination. The developed TA-loaded microemulsion showed superior therapeutic efficiency in the treatment of uveitis with high patient compliance compared to commercial suspension. Hence, it could be considered as a potential ocular treatment option in controlling of uveitis.

In vivo evaluation of a new hydrophobic acrylic intraocular lens in the rabbit model

2018 ◽  
Vol 44 (12) ◽  
pp. 1497-1502 ◽  
Author(s):  
Liliana Werner ◽  
Nathan Ellis ◽  
Joshua Bo Heczko ◽  
Marcia Ong ◽  
Rakhi Jain ◽  
...  
Keyword(s):  
Intraocular Lens ◽  
Rabbit Model ◽  
In Vivo Evaluation ◽  
Hydrophobic Acrylic

scholarly journals In Vivo Evaluation of the Mechanical Strength of a Slide Lengthening Technique With a Locking Mechanism Using a Rabbit Model

Cureus ◽  
2020 ◽  
Author(s):  
Dai Iwase ◽  
Kentaro Uchida ◽  
Yukie Metoki ◽  
Hiroyuki Sekiguchi ◽  
Jun Aikawa ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Rabbit Model ◽  
In Vivo Evaluation ◽  
Locking Mechanism

scholarly journals In vivo evaluation of the new PHIL low viscosity in a swine rete mirabile model

2018 ◽  
Vol 24 (6) ◽  
pp. 706-712 ◽  
Author(s):  
Edgar A Samaniego ◽  
Colin P Derdeyn ◽  
Minako Hayakawa ◽  
David Hasan ◽  
Santiago Ortega-Gutierrez
Keyword(s):  
Histological Analysis ◽  
Embolic Agent ◽  
In Vivo Evaluation ◽  
Rete Mirabile ◽  
Low Viscosity ◽  
Liquid Embolic ◽  
Optimal Average ◽  
The Right ◽  
Inflammatory Changes

Introduction Few liquid embolic materials are available for treatment of arteriovenous malformations. We describe the in vivo experience with the new PHIL low viscosity (LV) liquid embolic agent in a swine rete mirabile model. Methods Eight swine were treated. Two animals underwent embolization of a rete with PHIL LV and the contralateral rete with Squid 12 (euthanized the same day). Six animals underwent embolization of the right rete: two with balloon flow arrest (euthanized at 14 d) and four with a microcatheter alone (euthanized at 14 and 90 d). Performance characteristics of the embolic agents were evaluated. Microscopic and histological analysis of the harvested retia was performed. Macroscopic examinations and high contrast digital-based radiographs of the central nervous system were obtained. Results We did not experience any technical complication during embolization of each rete. Overall occlusion ability, on/off injection and ease to retrieve the microcatheter/balloon with PHIL LV were optimal. Fluoroscopic visualization of the PHIL LV cast was adequate to optimal. Average embolization time with flow arrest was 9.5 min versus 19.5 min with microcatheter plugging. Embolizations with PHIL LV required less volume and were shorter when compared to Squid 12. Subacute (14 d) and chronic (90 d) microscopic and histological analysis demonstrated minimal inflammatory changes in the perivascular tissues and permanent occlusion of the embolized vasculature. Conclusion In this swine rete model, the new PHIL LV embolic agent had an excellent embolization performance. Vessels embolized remained occluded up to 90 d from the procedure with minimal inflammatory changes.

In Vivo Evaluation of Nano-HA/PDLLA Composite

2005 ◽  
Vol 284-286 ◽  
pp. 881-884 ◽  
Author(s):  
Yu Mei Xiao ◽  
Hong Song Fan ◽  
Yao Wu ◽  
Jin Rui Xu ◽  
Y. Tan ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Sem Analysis ◽  
Albino Rats ◽  
Degradation Behavior ◽  
Porous Network ◽  
In Vivo Evaluation ◽  
Good Biocompatibility ◽  
Wistar Albino Rats ◽  
And Control

The purpose of this study was to evaluate the behavior of nano-hydroxyapatite/ poly(D,L)lactide (n-HA/PDLLA) composite in vivo. The composite rods containing about 40wt% n-HA and control HA rods with a diameter of 2mm and a length of 6mm were implanted into the femora of 16 New Zealand rabbits. Composite wafers with a diameter of 5mm and a thickness of 1mm were implanted into the dorsal subcutis of 18 Wistar Albino rats. After definite intervals, the histological analysis was completed by light microscopy and the degradation behavior was observed by scanning electron microscopy. The histological analysis showed no obvious difference between n-HA /PDLLA composite and pure HA that had good biocompatibility and osteoconductivity. SEM analysis of the surface and cross section of the samples showed that the degradation of the composite started from surface, then into the inner gradually and formed multiple pores at surface. The pore size and porosity gradually increased along with time and a porous network may be formed.

Bone regeneration strategy by different sized multichanneled biphasic calcium phosphate granules: In vivo evaluation in rabbit model

2018 ◽  
Vol 32 (10) ◽  
pp. 1406-1420 ◽  
Author(s):  
Mirana Taz ◽  
Sang Ho Bae ◽  
Hae Il Jung ◽  
Hyun-Deuk Cho ◽  
Byong-Taek Lee
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Rabbit Model ◽  
Morphological Characteristics ◽  
Confocal Imaging ◽  
Bone Tissue Regeneration ◽  
Micro Computed Tomography ◽  
In Vivo Evaluation

A variety of synthetic materials are currently in use as bone substitutes, among them a new calcium phosphate-based multichannel, cylindrical, granular bone substitute that is showing satisfactory biocompatibility and osteoconductivity in clinical applications. These cylindrical granules differ in their mechanical and morphological characteristics such as size, diameter, surface area, pore size, and porosity. The aim of this study is to investigate whether the sizes of these synthetic granules and the resultant inter-granular spaces formed by their filling critical-sized bone defects affect new bone formation characteristics and to determine the best formulations from these individual types by combining the granules in different proportions to optimize the bone tissue regeneration. We evaluated two types of multichanneled cylindrical granules, 1 mm and 3 mm in diameter, combined the granules in two different proportions (wt%), and compared their different mechanical, morphological, and in vitro and in vivo biocompatibility characteristics. We assessed in vitro biocompatibility and cytotoxicity using MC3T3-E1 osteoblast-like cells using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal imaging. In vivo investigation in a rabbit model indicated that all four samples formed significantly better bone than the control after four weeks and eight weeks of implantation. Micro-computed tomography analysis showed more bone formation by the 1 mm cylindrical granules with 160 ± 10 µm channeled pore and 50% porosity than the other three samples ( p<.05), which we confirmed by histological analysis.

scholarly journals In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent

Bioimpacts ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Cécilia Delattre ◽  
Diego Velazquez ◽  
Caroline Roques ◽  
Graciela Pavon-Djavid ◽  
Véronique Ollivier ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Neointimal Hyperplasia ◽  
Rabbit Model ◽  
Metallic Stent ◽  
In Vivo Evaluation ◽  
Endothelial Colony Forming Cells ◽  
Cell Coverage ◽  
Stent Coating

Introduction: The major complications of stent implantation are restenosis and late stent thrombosis. PBMA polymers are used for stent coating because of their mechanical properties. We previously synthesized and characterized Dextrangraft-polybutylmethacrylate copolymer (Dex-PBMA) as a potential stent coating. In this study, we evaluated the haemocompatibility and biocompatibility properties of Dex-PBMA in vitro and in vivo. Methods: Here, we investigated: (1) the effectiveness of polymer coating under physiological conditions and its ability to release Tacrolimus®, (2) the capacity of Dex-PBMA to inhibit Staphylococcus aureus adhesion, (3) the thrombin generation and the human platelet adhesion in static and dynamic conditions, (4) the biocompatibility properties in vitro on human endothelial colony forming cells ( ECFC) and on mesenchymal stem cells (MSC) and in vivo in rat models, and (5) we implanted Dex-PBMA and Dex-PBMATAC coated stents in neointimal hyperplasia restenosis rabbit model. Results: Dex-PBMA coating efficiently prevented bacterial adhesion and release Tacrolimus®. Dex-PBMA exhibit haemocompatibility properties under flow and ECFC and MSC compatibility. In vivo, no pathological foreign body reaction was observed neither after intramuscular nor intravascular aortic implantation. After Dex-PBMA and Dex-PBMATAC coated stents 30 days implantation in a restenosis rabbit model, an endothelial cell coverage was observed and the lumen patency was preserved. Conclusion: Based on our findings, Dex-PBMA exhibited vascular compatibility and can potentially be used as a coating for metallic coronary stents.

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