scholarly journals Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

2017 ◽  
Vol Volume 12 ◽  
pp. 3207-3220 ◽  
Author(s):  
Johanna Poller ◽  
Jan Zaloga ◽  
Eveline Schreiber ◽  
Harald Unterweger ◽  
Christina Janko ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Iron Oxide ◽  
Cancer Treatment ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Breast Cancer Treatment ◽  
In Vitro Cytotoxicity ◽  
Oxide Nanoparticles ◽  
Selection Of

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

Mass Imaging of Iron Oxide Nanoparticles Inside Cells for In Vitro Cytotoxicity

2011 ◽  
Vol 11 (1) ◽  
pp. 638-641 ◽  
Author(s):  
Hyun Kyong Shon ◽  
Jungsin Park ◽  
Inhong Choi ◽  
Hyun Min Park ◽  
Dae Won Moon ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Oxide Nanoparticles

scholarly journals Development, Characterization and In Vitro Evaluation of Paclitaxel and Anastrozole Co-Loaded Liposome

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1110
Author(s):  
Minh Thanh Vu ◽  
Dinh Tien Dung Nguyen ◽  
Ngoc Hoi Nguyen ◽  
Van Thu Le ◽  
The Nam Dao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Combination Therapy ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Breast Cancer Treatment ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Studies ◽  
Combined Drugs

Paclitaxel (PTX) and anastrozole (ANA) have been frequently applied in breast cancer treatment. PTX is well-known for its anti-proliferative effect meanwhile ANA has just been discovered to act as an estrogen receptor α (ERα) ligand. The combination therapy of PTX and ANA is expected to improve treating efficiency, as ANA would act as a ligand binding with the ERα gene expressed in breast cancer cells and thereafter PTX would inhibit the division and cause death to those cancer cells. In this study, liposome-based nanocarriers (LP) were developed for co-encapsulation of PTX and ANA to improve the efficacy of the combined drugs in an Estrogen receptor-responsive breast cancer study. PTX-ANA co-loaded LP was prepared using thin lipid film hydration method and was characterized for morphology, size, zeta potential, drug encapsulation and in vitro drug release. In addition, cell proliferation (WST assay) and IN Cell Analyzer were used for in vitro cytotoxicity studies on a human breast cancer cell line (MCF-7). Results showed that the prepared LP and PTX-ANA-LP had spherical vesicles, with a mean particle size of 170.1 ± 13.5 nm and 189.0 ± 22.1 nm, respectively. Controlled and sustained releases were achieved at 72 h for both of the loaded drugs. The in vitro cytotoxicity study found that the combined drugs showed higher toxicity than each single drug separately. These results suggested a new approach to breast cancer treatment, consisting of the combination therapy of PTX and ANA in liposomes based on ER response.

In vitro selection of RNA sequences capable of mediating the formation of iron oxide nanoparticles

2009 ◽  
Vol 19 (44) ◽  
pp. 8320 ◽  
Author(s):  
Carly J. Carter ◽  
Magda Dolska ◽  
Alina Owczarek ◽  
Christopher J. Ackerson ◽  
Bruce E. Eaton ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
In Vitro Selection ◽  
Oxide Nanoparticles ◽  
Rna Sequences ◽  
Selection Of

Evaluation of multilayer coated magnetic nanoparticles as biocompatible curcumin delivery platforms for breast cancer treatment

RSC Advances ◽  
2015 ◽  
Vol 5 (107) ◽  
pp. 88096-88107 ◽  
Author(s):  
Mohammad Akrami ◽  
Mehdi Khoobi ◽  
Masoud Khalilvand-Sedagheh ◽  
Ismaeil Haririan ◽  
Abbas Bahador ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Cancer Treatment ◽  
Cellular Uptake ◽  
Breast Cancer Treatment ◽  
Release Profile ◽  
Iron Oxide Magnetic Nanoparticles ◽  
Layer Iron

A novel biocompatible multi-layer iron oxide magnetic nanoparticles with sustained sensitive release profile, and improved cellular uptake.

scholarly journals Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2799
Author(s):  
Iulia Ioana Lungu ◽  
Simona Nistorescu ◽  
Mădălina Andreea Badea ◽  
Andreea-Mihaela Petre ◽  
Ana-Maria Udrea ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
Oxide Nanoparticles ◽  
Laser Pyrolysis ◽  
Biological Studies

Even today, breast cancer remains a global public problem, with a high mortality rate among women. Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. By using iron oxide nanoparticles for cancer treatment, several advantages were highlighted: the ability to target specific locations derived from their magnetic properties and reduced side effects. The aim of this study was to examine on breast cancer cell line the anticancer potential of γ-Fe2O3 NPs loaded with doxorubicin (DOX) and stabilized with carboxymethylcellulose sodium (CMCNa). The γ-Fe2O3 NPs were synthesized by laser pyrolysis technique and their nanometric size and crystallinity were confirmed by X-ray diffraction and transmission electron microscopy. The loading efficiency was estimated by using absorption and fluorescence spectroscopy. The DOX conjugated//CMCNa coated γ-Fe2O3 NPs proved through the biological studies to have a good anticancer effect through the inhibition of tumoral cell proliferation, disruption of the cellular membrane, induction of cell death and reduced effects on normal breast cells. Our data showed that DOX cytotoxicity increases significantly when conjugated with ɣ-Fe2O3 and ɣ-Fe2O3_CMCNa, a 50% reduction of cancer cell viability was obtained with a concentration around 0.1 µg/mL.

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