scholarly journals MDM2–MDM4 molecular interaction investigated by atomic force spectroscopy and surface plasmon resonance

2016 ◽  
Vol Volume 11 ◽  
pp. 4221-4229 ◽  
Author(s):  
Salvatore Cannistraro ◽  
Ilaria Moscetti ◽  
Emanuela Teveroni ◽  
Fabiola Moretti ◽  
Anna Rita Bizzarri
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Force Spectroscopy ◽  
Atomic Force Spectroscopy ◽  
Atomic Force

Interaction of mutant p53 with p73: A Surface Plasmon Resonance and Atomic Force Spectroscopy study

2014 ◽  
Vol 1840 (6) ◽  
pp. 1958-1964 ◽  
Author(s):  
Simona Santini ◽  
Silvia Di Agostino ◽  
Emilia Coppari ◽  
Anna Rita Bizzarri ◽  
Giovanni Blandino ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Force Spectroscopy ◽  
Mutant P53 ◽  
Spectroscopy Study ◽  
Atomic Force Spectroscopy ◽  
Atomic Force

scholarly journals Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Duygu Çimen ◽  
Nilay Bereli ◽  
Adil Denizli
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Kinetic Studies ◽  
Force Microscopy ◽  
Spr Sensor ◽  
Atomic Force ◽  
Analysis Technique ◽  
Molecular Imprinting Technology

In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.

Probing protein–peptide–protein molecular architecture by atomic force microscopy and surface plasmon resonance

The Analyst ◽  
2000 ◽  
Vol 125 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Molly M. Stevens ◽  
Stephanie Allen ◽  
Martyn C. Davies ◽  
Clive J. Roberts ◽  
Saul J. B. Tendler ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Architecture ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Lectins at Interfaces—An Atomic Force Microscopy and Multi-Parameter-Surface Plasmon Resonance Study

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2348 ◽  
Author(s):  
Katrin Niegelhell ◽  
Thomas Ganner ◽  
Harald Plank ◽  
Evelyn Jantscher-Krenn ◽  
Stefan Spirk
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Protein Adsorption ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Spectroscopy ◽  
Carbohydrate Binding ◽  
Force Microscopy ◽  
Atomic Force ◽  
Slow Kinetics

Lectins are a diverse class of carbohydrate binding proteins with pivotal roles in cell communication and signaling in many (patho)physiologic processes in the human body, making them promising targets in drug development, for instance, in cancer or infectious diseases. Other applications of lectins employ their ability to recognize specific glycan epitopes in biosensors and glycan microarrays. While a lot of research has focused on lectin interaction with specific carbohydrates, the interaction potential of lectins with different types of surfaces has not been addressed extensively. Here, we screen the interaction of two specific plant lectins, Concanavalin A and Ulex Europaeus Agglutinin-I with different nanoscopic thin films. As a control, the same experiments were performed with Bovine Serum Albumin, a widely used marker for non-specific protein adsorption. In order to test the preferred type of interaction during adsorption, hydrophobic, hydrophilic and charged polymer films were explored, such as polystyrene, cellulose, N,-N,-N-trimethylchitosan chloride and gold, and characterized in terms of wettability, surface free energy, zeta potential and morphology. Atomic force microscopy images of surfaces after protein adsorption correlated very well with the observed mass of adsorbed protein. Surface plasmon resonance spectroscopy studies revealed low adsorbed amounts and slow kinetics for all of the investigated proteins for hydrophilic surfaces, making those resistant to non-specific interactions. As a consequence, they may serve as favorable supports for biosensors, since the use of blocking agents is not necessary.

scholarly journals Nanostructured Chitosan/Maghemite Composites Thin Film for Potential Optical Detection of Mercury Ion by Surface Plasmon Resonance Investigation

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1497
Author(s):  
Nurul Illya Muhamad Fauzi ◽  
Yap Wing Fen ◽  
Nur Alia Sheh Omar ◽  
Silvan Saleviter ◽  
Wan Mohd Ebtisyam Mustaqim Mohd Daniyal ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mercury Ion ◽  
Resonance Spectroscopy ◽  
Force Microscopy ◽  
Sensing Applications ◽  
Atomic Force ◽  
Fe2o3 Thin Film

In this study, synthesis and characterization of chitosan/maghemite (Cs/Fe2O3) composites thin film has been described. Its properties were characterized using Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet-visible spectroscopy (UV-Vis). FTIR confirmed the existence of Fe–O bond, C–N bond, C–C bond, C–O bond, O=C=O bond and O–H bond in Cs/Fe2O3 thin film. The surface morphology of the thin film indicated the relatively smooth and homogenous thin film, and also confirmed the interaction of Fe2O3 with the chitosan. Next, the UV-Vis result showed high absorbance value with an optical band gap of 4.013 eV. The incorporation of this Cs/Fe2O3 thin film with an optical-based method, i.e., surface plasmon resonance spectroscopy showed positive response where mercury ion (Hg2+) can be detected down to 0.01 ppm (49.9 nM). These results validate the potential of Cs/Fe2O3 thin film for optical sensing applications in Hg2+ detection.

scholarly journals Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

2017 ◽  
Vol 10 (02) ◽  
pp. 1650040 ◽  
Author(s):  
Tao Ma ◽  
Xiaoxia Chen ◽  
Qing Peng ◽  
Pengfei Zhang ◽  
Yonghong He
Keyword(s):  
Aqueous Solution ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Surface Functionalization ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Flow Condition ◽  
Interaction Analysis ◽  
Low Cost ◽  
Protein A

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

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