scholarly journals Novel antimicrobial peptide–modified azithromycin-loaded liposomes against methicillin-resistant Staphylococcus aureus

2016 ◽  
Vol Volume 11 ◽  
pp. 6781-6794 ◽  
Author(s):  
Xiaowei Liu ◽  
Zhan Li ◽  
Xiaodong Wang ◽  
Yujuan Chen ◽  
Fengbo Wu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Methicillin Resistant

scholarly journals Melittin, a honeybee venom-derived antimicrobial peptide, may target methicillin-resistant Staphylococcus aureus

2015 ◽  
Vol 12 (5) ◽  
pp. 6483-6490 ◽  
Author(s):  
JI HAE CHOI ◽  
A YEUNG JANG ◽  
SHUNMEI LIN ◽  
SANGYONG LIM ◽  
DONGHO KIM ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Honeybee Venom ◽  
Methicillin Resistant

scholarly journals Use of the Antimicrobial Peptide Pardaxin (GE33) To Protect against Methicillin-Resistant Staphylococcus aureus Infection in Mice with Skin Injuries

2013 ◽  
Vol 58 (3) ◽  
pp. 1538-1545 ◽  
Author(s):  
Han-Ning Huang ◽  
Chieh-Yu Pan ◽  
Yi-Lin Chan ◽  
Jyh-Yih Chen ◽  
Chang-Jer Wu
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Bacterial Infections ◽  
Lethal Dose ◽  
Antimicrobial Activities ◽  
Methicillin Resistant ◽  
Content Type ◽  
Antimicrobial Function ◽  
Monocytes And Macrophages ◽  
Endothelial Growth Factor

ABSTRACTAntimicrobial peptides (AMPs) have recently been determined to be potential candidates for treating drug-resistant bacterial infections. Pardaxin (GE33), a marine antimicrobial peptide, has been reported to possess antimicrobial function. In this study, we investigated whether pardaxin promoted healing of contaminated wounds in mice. One square centimeter of outer skin was excised from the ventral region of mice, and a lethal dose of methicillin-resistantStaphylococcus aureus(MRSA) was applied in the presence or absence of methicillin, vancomycin, or pardaxin. While untreated mice and mice treated with methicillin died within 3 days, mice treated with pardaxin survived infection. Pardaxin decreased MRSA bacterial counts in the wounded region and also enhanced wound closure. Reepithelialization and dermal maturation were also faster in mice treated with pardaxin than in mice treated with vancomycin. In addition, pardaxin treatment controlled excess recruitment of monocytes and macrophages and increased the expression of vascular endothelial growth factor (VEGF). In conclusion, these results suggest that pardaxin is capable of enhancing wound healing. Furthermore, this study provides an excellent platform for comparing the antimicrobial activities of peptide and nonpeptide antibiotics.

scholarly journals Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251514
Author(s):  
Mohamad Malik Al-adil Baharudin ◽  
Mohamad Syazwan Ngalimat ◽  
Fairolniza Mohd Shariff ◽  
Zetty Norhana Balia Yusof ◽  
Murni Karim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
High Performance ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Proteinase K ◽  
Tween 20 ◽  
Bacillus Velezensis ◽  
Promising Alternative ◽  
Methicillin Resistant

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have reached epidemic proportions globally. Therefore, there is an urgent need for a continuous supply of antibiotics to combat the problem. In this study, bacteria initially identified as species belonging to the Bacillus amyloliquefaciens operational group were re-identified based on the housekeeping gene, gyrB. Cell-free supernatants (CFS) from the strains were used for antimicrobial tests using the agar well diffusion assay against MRSA and various types of pathogenic bacteria. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and physicochemical characteristics of the CFS were determined. Based on gyrB sequence analysis, five strains (PD9, B7, PU1, BP1 and L9) were identified as Bacillus velezensis. The CFS of all B. velezensis strains showed broad inhibitory activities against Gram-negative and -positive as well as MRSA strains. Strain PD9 against MRSA ATCC 33742 was chosen for further analysis as it showed the biggest zone of inhibition (21.0 ± 0.4 mm). The MIC and MBC values obtained were 125 μl/ml. The crude antimicrobial extract showed bactericidal activity and was stable at various temperatures (40–80°C), pH (4–12), surfactants (Tween 20, Tween 80, SDS and Triton X-100) and metal ions (MgCI2, NaCI2, ZnNO3 and CuSO4) when tested. However, the crude extract was not stable when treated with proteinase K. All these properties resembled the characteristics of peptides. The antimicrobial compound from the selected strain was purified by using solvent extraction method and silica gel column chromatography. The purified compound was subjected to High Performance Liquid Chromatography which resulted in a single peak of the anti-MRSA compound being detected. The molecular weight of the anti-MRSA compound was determined by using SDS-PAGE and zymogram. The size of the purified antimicrobial peptide was approximately ~ 5 kDa. The antimicrobial peptide produced from B. velezensis strain PD9 is a promising alternative to combat the spread of MRSA infections in the future.

In vitro activity of the antimicrobial peptide AP7121 against the human methicillin-resistant biofilm producers Staphylococcus aureus and Staphylococcus epidermidis

Biofouling ◽  
2020 ◽  
Vol 36 (3) ◽  
pp. 266-275
Author(s):  
Gastón Delpech ◽  
Mónica Ceci ◽  
Sabina Lissarrague ◽  
Leonardo García Allende ◽  
Beatriz Baldaccini ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptide ◽  
Staphylococcus Epidermidis ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Methicillin Resistant
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