scholarly journals Histopathological changes induced in an animal model by potentially pathogenic Enterococcus faecalis strains recovered from ready-to-eat food outlets in Osun State, Nigeria

2015 ◽  
pp. 181
Author(s):  
O Famurewa ◽  
Adetunji Olawale ◽  
Oluwole David ◽  
Adekemi Oluyege ◽  
Richard Osuntoyinbo ◽  
...  
Keyword(s):  
Animal Model ◽  
Enterococcus Faecalis ◽  
Histopathological Changes ◽  
Food Outlets

scholarly journals A Novel Swine Model to Evaluate Arterial Vessel Injury after Mechanical Endovascular Thrombectomy

2013 ◽  
Vol 19 (2) ◽  
pp. 147-152 ◽  
Author(s):  
B. Gory ◽  
D. Bresson ◽  
A. Rouchaud ◽  
C. Yardin ◽  
C. Mounayer
Keyword(s):  
Animal Model ◽  
Vessel Wall ◽  
Arterial Injury ◽  
Swine Model ◽  
Histopathological Changes ◽  
Endovascular Thrombectomy ◽  
Vessel Injury ◽  
Intracranial Arteries ◽  
Device Interaction

Few animal models have been reported to evaluate and compare mechanical endovascular thrombectomy (MET) devices used to treat human ischemic stroke. These models may contribute to the understanding of arterial injury induced by a MET device and potentially by extrapolation to human intracranial arteries. We have developed a novel swine model for MET that allows visualization of the thrombus/device interaction and characterization of mechanical impact on the vessel wall. Twenty superficial femoral arteries were occluded with radiopaque thrombus, and 20 without thrombus were treated with thrombectomy devices. Acute histopathological changes were evaluated. The swine femoral artery, which is comparable in size to the human middle cerebral artery or basilar artery, may offer a useful animal model for the study of histologic alterations induced by MET.

scholarly journals Gambaran Histopatologik Paru pada Hewan Coba Postmortem

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Sekar N. Ali ◽  
Sunny Wangko ◽  
Sonny J.R. Kalangi
Keyword(s):  
Animal Model ◽  
Smooth Muscle ◽  
Observational Study ◽  
Histological Change ◽  
Postmortem Changes ◽  
Time Of Death ◽  
Histopathological Changes ◽  
Alveolar Cells ◽  
Time Intervals ◽  
Histological Changes

Abstract: Postmortem changes play an important role in estimation of the time of death. This study was aimed to obtain the histopathological changes of lungs at several time intervals postmortem. This was a descriptive observational study using a local pig as the animal model killed by stabbing in the heart. Lung samples were taken at 15 minutes, 30 minutes, 45 minutes, 60 minutes, 75 minutes, 90 minutes, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 12 hours, and 24 hours postmortem. The results showed that the earliest histological change could be identified at 60 minutes postmortem in the form of alveolar dilatation. At 2 hours postmortem, congestion of smooth muscle layers of bronchioles was observed. The epithelial cells of the alveoli were undetected at 3 hours postmortem meanwhile the smooth muscle layers were undetected at 12 hours postmortem. At 24 hours postmortem, the bronchioles were still detected but the structure of their layers could not be identified. Conclusion: Histopathological changes were observed as alveolar dilatation at 60 minutes postmortem, followed by congestion of muscle layers, and undetected epithelial alveolar cells as well as smooth muscle layers. At 24 hours postmortem, bronchioles were still detected but the structure of their layers could not be identified.Keywords: histological changes, lungs, postmorterm Abstrak: Perubahan postmortem berperan penting untuk memperkirakan waktu kematian. Penelitian ini bertujuan untuk mendapatkan perubahan histopatologik paru hewan coba postmortem pada beberapa interval waktu. Jenis penelitian ialah deskriptif-observasional menggunakan hewan coba satu ekor babi lokal yang dimatikan dengan tikaman pada jantung. Sampel paru diambil dalam waktu 15 menit, 30 menit, 45 menit, 60 menit, 75 menit, 90 menit, 2 jam, 3 jam, 4 jam, 5 jam, 6 jam, 12 jam dan 24 jam postmortem. Hasil penelitian mendapatkan pada 60 menit postmortem terjadi dilatasi alveoli. Pada 2 jam postmortem tampak kongesti lapisan otot polos. Pada 3 jam postmortem sel-sel epitel alveoli tidak tampak lagi dan pada 12 jam postmortem lapisan otot polos tidak terdeteksi. Pada 24 jam postmortem bronkiolus masih dapat dideteksi tetapi struktur lapisannya tidak teridentifikasi. Simpulan: Perubahan awal histopatologik paru babi postmotem ini dimulai pada 60 menit postmortem berupa dilatasi alveoli, diikuti kongesti lapisan otot polos, serta tidak terdeteksinya sel-sel epitel alveoli dan lapisan otot polos. Pada 24 jam postmortem bronkiolus masih terdeteksi tetapi struktur lapisannya tidak teridentifikasi lagi.Kata kunci: perubahan histologik, paru, postmortem

scholarly journals Group A Rotavirus Infection and Age-Dependent Diarrheal Disease in Rats: a New Animal Model To Study the Pathophysiology of Rotavirus Infection

2002 ◽  
Vol 76 (1) ◽  
pp. 41-57 ◽  
Author(s):  
Max Ciarlet ◽  
Margaret E. Conner ◽  
Milton J. Finegold ◽  
Mary K. Estes
Keyword(s):  
Animal Model ◽  
Small Intestine ◽  
Virus Antigen ◽  
Neonatal Rats ◽  
Histopathological Changes ◽  
Rotavirus Infection ◽  
Age Dependent ◽  
Group A Rotaviruses ◽  
Group A ◽  
Old Rats

ABSTRACT Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, WI61, and HAL1166), simian (rhesus rotavirus [RRV] and SA11), bovine (WC3), lapine (ALA), or porcine (OSU) rotavirus strains, wild-type murine (ECwt) rotavirus strain, or phosphate-buffered saline (PBS). Rotavirus infection in rats was evaluated by (i) clinical findings, (ii) virus antigen shedding or infectious virus titers in the feces or intestinal contents measured by enzyme-linked immunosorbent assay or fluorescent-focus assay, (iii) histopathological changes in the small intestine, (iv) distribution of rotavirus antigen in small-intestine sections by immunofluorescence, and (v) growth rate. Rotavirus infection of 5-day-old but not ≥21-day-old rats resulted in diarrhea that lasted from 1 to 10 days postinoculation. The severity of disease and spread of infection to naÏve littermates differed depending on the virus strain used for inoculation. The duration of virus antigen shedding following infection was considerably prolonged (up to 10 days) in neonatal rats compared to that in 21-day-old rats (1 or 2 days). Based on lack of virus antigen shedding and disease induction, the murine ECwt rotavirus was the only strain tested that did not infect rats. Histopathological changes in the small-intestine mucosa of 5-day-old RRV-inoculated rats but not of PBS-inoculated rats was limited to extensive enterocyte vacuolation in the ileum. In RRV-inoculated neonatal rats, rotavirus antigen was detected in the epithelial cells on the upper half of the intestinal villi of the jejunum and ileum. In addition, infection of neonatal rats with RRV but not with PBS resulted in reduced weight gain. Rats infected with group A rotaviruses provide a new animal model with unique features amenable to investigate rotavirus pathogenesis and the molecular mechanisms of intestinal development, including physiological factors that may regulate age-dependent rotavirus-induced diarrhea.

In Vivo Biocompatibility Studies of Nano TiO2 Materials

2009 ◽  
Vol 79-82 ◽  
pp. 389-392 ◽  
Author(s):  
Wei Han ◽  
Yue Dan Wang ◽  
Yu Feng Zheng
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Catalase Activity ◽  
Histopathological Changes ◽  
Vacuolar Degeneration ◽  
Tio2 Nanomaterials ◽  
Liver And Kidney ◽  
Different Dimensions ◽  
And Oxidative Stress

TiO2 nanomaterials with different dimensions(zero and one), sizes(20nm, 50nm and 100nm in diameter) and crystal structures(100% rutile, 100% anatase and combination of 20% rutile and 80% anatase) were confected to suspensions and ointment with varied concentrations and evaluated in animal model (Balb-c mouse). These mouse were divided into various groups randomly, with suspension intraperitoneally injected or ointment transdermally daubed. Heart, lung, liver and kidney were collected and prepared to HE sample after one week. Spectrophotometry was applied to study total antioxide capability and catalase activity of blood and tissues. It has been shown that all TiO2 nanomaterial groups had no effect on lives’ morphology and oxidative stress, with no obvious histopathological changes observed in heart, lung, liver and kidney, and these tissues presented no vacuolar degeneration, necrosis edema, engorgement and inflammation.

scholarly journals Transfer of the Pheromone-Inducible Plasmid pCF10 among Enterococcus faecalis Microorganisms Colonizing the Intestine of Mini-Pigs

2002 ◽  
Vol 68 (1) ◽  
pp. 187-193 ◽  
Author(s):  
Tine Rask Licht ◽  
Dorthe Laugesen ◽  
Lars Bogø Jensen ◽  
Bodil Lund Jacobsen
Keyword(s):  
Animal Model ◽  
Enterococcus Faecalis ◽  
Recipient Strain ◽  
Donor Strain ◽  
Fecal Samples ◽  
Donor Cells ◽  
Resistance Markers ◽  
Resistant Strains ◽  
Selective Plating ◽  
Mini Pigs

ABSTRACT A new animal model, the streptomycin-treated mini-pig, was developed in order to allow colonization of defined strains of Enterococcus faecalis in numbers sufficient to study plasmid transfer. Transfer of the pheromone-inducible pCF10 plasmid between streptomycin-resistant strains of E. faecalis OG1 was investigated in the model. The plasmid encodes resistance to tetracycline. Numbers of recipient, donor, and transconjugant bacteria were monitored by selective plating of fecal samples, and transconjugants were subsequently verified by PCR. After being ingested by the mini-pigs, the recipient strain persisted in the intestine at levels between 106 and 107 CFU per g of feces throughout the experiment. The donor strain, which carried different resistance markers but was otherwise chromosomally isogenic to the recipient strain, was given to the pigs 3 weeks after the recipient strain. The donor cells were initially present in high numbers (106 CFU per g) in feces, but they did not persist in the intestine at detectable levels. Immediately after introduction of the donor bacteria, transconjugant cells appeared and persisted in fecal samples at levels between 103 and 104 CFU per g until the end of the experiment. These observations showed that even in the absence of selective tetracycline pressure, plasmid pCF10 was transferred from ingested E. faecalis cells to other E. faecalis organisms already present in the intestinal environment and that the plasmid subsequently persisted in the intestine.

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