scholarly journals Rapid Identification of KL49 Acinetobacter baumannii Associated with Clinical Mortality

2020 ◽  
Vol Volume 13 ◽  
pp. 4125-4132
Author(s):  
Qiuyang Deng ◽  
Jinyong Zhang ◽  
Min Zhang ◽  
Zhou Liu ◽  
Yuxin Zhong ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Rapid Identification

scholarly journals Rapid identification of Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii with a multiplex PCR assay

2014 ◽  
Vol 63 (9) ◽  
pp. 1154-1159 ◽  
Author(s):  
Te-Li Chen ◽  
Yi-Tzu Lee ◽  
Shu-Chen Kuo ◽  
Su-Pen Yang ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Acinetobacter Calcoaceticus ◽  
Rapid Identification ◽  
23S Rrna ◽  
Pcr Assay ◽  
Complex Species ◽  
Multiplex Pcr Assay ◽  
Acinetobacter Pittii ◽  
Reference Strains

Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus–A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. In this study, we investigated the use of a multiplex PCR-based assay designed to detect internal fragments of the 16S–23S rRNA intergenic region and the gyrB and recA genes. The assay was capable of differentiating A. baumannii, A. nosocomialis and A. pittii in a reliable manner. In 23 different reference strains and 89 clinical isolates of Acinetobacter species, the assay accurately identified clinically relevant Acb complex species except those ‘between 1 and 3’ or ‘close to 13TU’. None of the non-Acb complex species was misidentified. In an analysis of 1034 positive blood cultures, the assay had a sensitivity of 92.4 % and specificity of 98.2 % for Acb complex identification. Our results show that a single multiplex PCR assay can reliably differentiate clinically relevant Acb complex species. Thus, this method may be used to better understand the clinical differences between infections caused by these species.

scholarly journals Rapid ultrasensitive diagnosis of pneumonia caused by Acinetobacter baumannii using a combination of enrichment and phage-based qPCR assay

2020 ◽  
Author(s):  
Jun Luo ◽  
Mengwei Jiang ◽  
Jin Xiong ◽  
Junhua Li ◽  
Hongping Wei ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Enrichment Culture ◽  
Detection System ◽  
Sputum Sample ◽  
High Sensitivity ◽  
Rapid Identification ◽  
Clinical Method ◽  
Real Time Quantitative Pcr ◽  
Live Bacteria ◽  
Infection Patient

Abstract Background Accurate and rapid identification of ventilator associated or nosocomial pneumonia caused by Acinetobacter baumannii ( A. baumannii ) could improve the treatment.Methods In current study, we developed a phage-based real-time quantitative PCR (qPCR) combined with enrichment culture for rapid and specific detection of viable A. baumannii in sputum from lung infections. Through short-term plate incubation, bacteria can be enriched and the DNA polymerase reaction disturbance of sputum can be decreased greatly. This approach is based on detecting phage replication in live A. baumannii cells through Taqman qPCR.Results Through the built detection system, down to 1 CFU of A. baumannii can be detected within 6 h in spiked sputum samples without any steps of bacteria isolation and DNA extraction. The established method was then applied to detecting both A. baumannii in simulated sputum with 100% agreement with the spiked amount of the bacteria and one clinical sputum sample from an 80-year-old male lung infection patient caused by A. baumannii with perfect accuracy, demonstrating that the assay developed in this study has the merits of high rapidity, high sensitivity, good specificity and being able to detect live bacteria not dead bacteria.Conclusions The assay is a potentially clinical method for diagnosis of bacterial pneumonia infection caused by A. baumannii or other bacterial infection in sputum or complicated samples through switching to other types of phages.

scholarly journals A Tractable Drosophila Cell System Enables Rapid Identification of Acinetobacter baumannii Host Factors

2020 ◽  
Vol 10 ◽  
Author(s):  
Qing-Ming Qin ◽  
Jianwu Pei ◽  
Gabriel Gomez ◽  
Allison Rice-Ficht ◽  
Thomas A. Ficht ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Rapid Identification ◽  
Cell System ◽  
Host Factors ◽  
Drosophila Cell

scholarly journals Strategies for Rapid Identification of Acinetobacter baumannii Membrane Proteins and Polymyxin B’s Effects

2021 ◽  
Vol 11 ◽  
Author(s):  
Yun Lu ◽  
Xinxin Hu ◽  
Tongying Nie ◽  
Xinyi Yang ◽  
Congran Li ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sample Preparation ◽  
Acinetobacter Baumannii ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Rapid Identification ◽  
Label Free ◽  
Membrane Proteome ◽  
Antibiotic Development ◽  
Digestion Methods

Acinetobacter baumannii, especially multidrug resistant Acinetobacter baumannii, is a notable source of pressure in the areas of public health and antibiotic development. To overcome this problem, attention has been focused on membrane proteins. Different digestion methods and extraction detergents were examined for membrane proteome sample preparation, and label-free quantitative and targeted proteome analyses of the polymyxin B-induced Acinetobacter baumannii ATCC 19606 membrane proteome were performed based on nano LC-MS/MS. Ultracentrifugation of proteins at a speed of 150,000×g, digestion by trypsin, filter-aided sample preparation, and detergents such as lauryldimethylamine-N-oxide were proved as a fast and effective way for identification of membrane proteome by nano LC-MS/MS. Upon treatment with polymyxin B, expression levels of 15 proteins related to membrane structure, transporters, cell surface, and periplasmic space were found to be significantly changed. Furthermore, targeted proteome was also used to confirm these changes. A relatively rapid membrane proteome preparation method was developed, and a more comprehensive view of changes in the Acinetobacter baumannii membrane proteome under polymyxin B pressure was obtained.

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