scholarly journals Characterization of Extended-Spectrum β-Lactamase-Producing Uropathogenic Escherichia coli Among Iranian Kidney Transplant Patients

2020 ◽  
Vol Volume 13 ◽  
pp. 1429-1437 ◽  
Author(s):  
Mehrdad Halaji ◽  
Shahrzad Shahidi ◽  
Abdolamir Atapour ◽  
Behrooz Ataei ◽  
Awat Feizi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kidney Transplant ◽  
Transplant Patients ◽  
Extended Spectrum ◽  
Kidney Transplant Patients

scholarly journals Molecular epidemiology of blaCTX-M gene-producing uropathogenic Escherichia coli among Iranian kidney transplant patients: clonal dissemination of CC131 and CC10

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Mehrdad Halaji ◽  
Shahrzad Shahidi ◽  
Behrooz Ataei ◽  
Abdolamir Atapour ◽  
Awat Feizi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kidney Transplant ◽  
Phylogenetic Group ◽  
Control Group ◽  
M Gene ◽  
Mlst Analysis ◽  
Phylogenetic Characterization ◽  
Transplant Patients ◽  
Kidney Transplant Patients

Abstract Background This study aimed to investigate the phylogenetic characterization and virulence traits of uropathogenic Escherichia coli (UPEC) isolated from kidney transplant patients (KTPs) as well as non-KTPs and analyze the clonal distribution of Extended spectrum β-lactamases (ESBLs)-producing UPEC containing blaCTX-M gene. Methods To this end, we determined virulence marker and the phylogenetic characterization of UPEC in non-KTPs (n = 65) and KTPs (n = 46). The non-KTPs were considered the control group of the study. Also, according to the Achtman scheme, we performed multilocus sequence typing to assess the relationship between twenty-nine of ESBL-producing isolates containing blaCTX-M gene. Results According to the results of PCR assay, the prevalence of virulence factor genes ranged from 0% (cnf and papG III) to 93.7% (fimH). Also, KTP isolates significantly differed from non-KTP isolates only in terms of the prevalence of pap GI elements. Moreover, the most frequent UPEC isolates were in phylogenetic group B2, followed by group D (18.9%), and group A (13.5%). Furthermore, except for phylogenetic group C, there was no significant correlation between phylogenetic distribution in KTPs and non-KTPs. Additionally, MLST analysis of blaCTX-M carrying isolates identified 18 unique sequence types (ST) the most common of which was ST131 (24.1%), followed by ST1193 (10.3%), while fourteen STs were detected only once. Conclusions The results further revealed significant differences between the UPEC isolates from KTPs and non-KTPs regarding the phylogroups C and PAI gene. Based on MLST analysis, we also observed a relatively high diversity in UPEC isolates obtained from KTPs and non-KTPs. Moreover, clonal complex (CC) 131 and ST131 were found to be the most prevalent clones and ST types, respectively. Besides, for the first time, ST8503 were reported in KTPs. These results suggested regular studies on characterization of UPEC isolates among KTPs.

scholarly journals Characterization of Plasmid-Mediated Quinolone Resistance and Serogroup Distributions of Uropathogenic Escherichia coli among Iranian Kidney Transplant Patients

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Amin Sadeghi ◽  
Mehrdad Halaji ◽  
Amirhossein Fayyazi ◽  
Seyed Asghar Havaei
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Kidney Transplant ◽  
Uropathogenic Escherichia Coli ◽  
Quinolone Resistance ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Transplant Patients ◽  
Kidney Transplant Patients ◽  
Plasmid Genes

Introduction. Urinary tract infection (UTI) is one of the most frequent infections in kidney transplant patients (KTPs). This infection is mainly caused by uropathogenic Escherichia coli (UPEC). Plasmid-mediated quinolone resistance (PMQR) was also increasingly identified in UPEC. This study proposed to investigate the frequency of quinolone-resistance plasmid genes and the O-antigen serogroup among UPEC isolated from KTPs and non-KTP with UTI. Methods. Totally, 114 UPEC isolates from 49 KTPs and 65 non-KTPs patients diagnosed with an UPEC-associated UTI were obtained from June 2019 to December 2019 at three laboratory centers in Isfahan, Iran. The isolates were confirmed through phenotypic and genotypic methods. Moreover, the antimicrobial susceptibility test to nalidixic acid, ciprofloxacin, norfloxacin, and ofloxacin was performed using a disk diffusion method. The presence of the qnr gene as well as the serogroup distribution was identified using the PCR method. Result. According to data, the distribution of O1, O2, O4, O16, and O25 serogroups were 3.5%, 2.6, 3.5, 3.5, and 20.2%, respectively. Antibiotic susceptibility pattern revealed that the highest and lowest resistance rates were to nalidixic acid (69.3%) and norfloxacin (43.9%), respectively. Also, the frequency of qnrS and qnrB genes were 33.3% and 15.8%, respectively, while none of the isolates was found to be positive for the qnrA gene. There was no significant association between the presence of qnr genes and higher antibiotic resistance. Conclusion. This study recognized that the qnrS gene, O25 serotype, and resistance to nalidixic acid had the highest frequencies in UPEC strains isolated from UTI patients.

Characterization of CD28−CD4+ T cells in living kidney transplant patients with long-term allograft acceptance

2001 ◽  
Vol 62 (12) ◽  
pp. 1335-1345 ◽  
Author(s):  
Masashi Kato ◽  
Tetsuya Matsuguchi ◽  
Yoshinari Ono ◽  
Ryohei Hattori ◽  
Shinichi Ohshima ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplant ◽  
Cd4 T Cells ◽  
Transplant Patients ◽  
Kidney Transplant Patients

CHARACTERIZATION OF CMVpp65-SPECIFIC CD8+ T LYMPHOCYTES USING MHC TETRAMERS IN KIDNEY TRANSPLANT PATIENTS AND HEALTHY PARTICIPANTS1

2000 ◽  
Vol 69 (11) ◽  
pp. 2243-2250 ◽  
Author(s):  
Mats Engstrand ◽  
Claire Tournay ◽  
Marie A. Peyrat ◽  
Britt-Marie Eriksson ◽  
Jonas Wadstr??m ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Kidney Transplant ◽  
Transplant Patients ◽  
Cd8 T Lymphocytes ◽  
Mhc Tetramers ◽  
Kidney Transplant Patients

Detection and Characterization of Atypical Renal Cysts and Solid Renal Masses in Kidney Transplant Patients by Use of Dual-Energy CT Iodine Maps

2019 ◽  
Vol 213 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Floriane L'Hostis ◽  
Jeremie Vial ◽  
Florine Dallery ◽  
Raphael Pichois ◽  
Nicolas Deleval ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Renal Cysts ◽  
Dual Energy ◽  
Dual Energy Ct ◽  
Renal Masses ◽  
Transplant Patients ◽  
Kidney Transplant Patients ◽  
Iodine Maps
Sign in / Sign up

Export Citation Format

Share Document