scholarly journals Molecular Detection Of Multidrug-Resistant Salmonella Isolated From Livestock Production Systems In South Africa

2019 ◽  
Vol Volume 12 ◽  
pp. 3537-3548 ◽  
Author(s):  
Thobeka P Mthembu ◽  
Oliver T Zishiri ◽  
Mohamed E El Zowalaty
Keyword(s):  
South Africa ◽  
Molecular Detection ◽  
Production Systems ◽  
Multidrug Resistant ◽  
Livestock Production

scholarly journals Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 124 ◽  
Author(s):  
Mthembu ◽  
Zishiri ◽  
Zowalaty
Keyword(s):  
South Africa ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Production Systems ◽  
Livestock Production ◽  
Small Scale ◽  
Foodborne Diseases ◽  
Sampling Location ◽  
Salmonella Spp ◽  
Zoonotic Infections

Livestock are an important source of protein and food for humans, however opportunistic pathogens such as Salmonella spp. turn livestock into vehicles of foodborne diseases. This study investigated the prevalence of virulence genes in Salmonella spp. isolated from livestock production systems in two provinces of South Africa. During the period from May to August, 2018, a total of 361 faecal (189), oral (100), environmental (soil (36) and water (27)) and feed (9) samples were randomly collected from different animals (cattle, sheep, goats, pigs, ducks and chickens) that were housed in small-scale livestock production systems from Eastern Cape and KwaZulu-Natal Provinces in South Africa. Salmonella spp. were isolated and identified using microbiological and DNA molecular methods. Salmonella spp. were present in 29.0% of the samples of which 30.2% belonged to the Salmonella enterica species as confirmed by the positive amplification of the species specific iroB gene. Virulence genes that were screened from livestock-associated Salmonella were invA, iroB, spiC, pipD and int1. Statistically significant associations (p < 0.05) were established between the virulence genes, sampling location, animal host as well as the season when samples were collected. Furthermore, statistically significant (p < 0.05) positive correlations were observed between most of the virulence genes investigated. This is one of the recent studies to detect and investigate livestock-associated Salmonella spp. in South Africa. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae. It also demonstrated the detection and prevalence of virulent Salmonella spp. harbored by livestock in South Africa. This study demonstrated the potential risks of pathogenic Salmonella enterica to cause foodborne diseases and zoonotic infections from farm-to-fork continuum using the global one-health approach.

Benefits obtained from cattle by smallholder farmers: a case study of Limpopo Province, South Africa

2008 ◽  
Vol 48 (7) ◽  
pp. 825 ◽  
Author(s):  
A. Stroebel ◽  
F. J. C. Swanepoel ◽  
N. D. Nthakheni ◽  
A. E. Nesamvuni ◽  
G. Taylor
Keyword(s):  
South Africa ◽  
Production Systems ◽  
Smallholder Farmers ◽  
Meat Consumption ◽  
Livestock Production ◽  
Limpopo Province ◽  
Wealth Status ◽  
Livestock Farmers ◽  
Vhembe District

A survey of livestock production systems was conducted in the Vhembe District, located between 22°85′ latitude and 30°71′ longitude in the Limpopo Province of South Africa. The objective of the study was to investigate the benefits obtained from, and key functions of, cattle production, as well as related breeding objectives in smallholder livestock production in the Limpopo Province of South Africa. Data were collected by means of a general survey questionnaire and a participatory rural appraisal (PRA) exercise. The results indicate that almost 60% of farmers own < 10 cattle. Female animals constituted the largest component of the herd (55.02%). Although the bull : cow ratio was extremely high (1 : 3.7), the calving rate was low at 35.60%, with an extremely high herd mortality of 15.70% and a low off-take of 8.70%. In a pairwise ranking, the benefits obtained from cattle by smallholder livestock farmers are as follows: selling and meat consumption (4), wealth, status and savings (3), socio-cultural activities (2) and draught power (1). The ‘cattle complex’, where cattle are kept for prestige and status, is still appropriate, but cattle also make significant contributions in respect to selling and meat consumption, and arable inputs such as draft and fertiliser. It is concluded that benefits obtained from cattle form the basis of decision-making by smallholder cattle owners with respect to livestock production.

scholarly journals Evaluating management options that use climate forecasts: modelling livestock production systems in the semi-arid zone of South Africa

2004 ◽  
Vol 26 ◽  
pp. 33-42 ◽  
Author(s):  
PK Thornton ◽  
RH Fawcett ◽  
KA Galvin ◽  
RB Boone ◽  
JW Hudson ◽  
...  
Keyword(s):  
South Africa ◽  
Production Systems ◽  
Arid Zone ◽  
Livestock Production ◽  
Management Options ◽  
Climate Forecasts ◽  
Semi Arid

scholarly journals Multidrug-Resistant Listeria Species Shows Abundance in Environmental Waters of a Key District Municipality in South Africa

2021 ◽  
Vol 18 (2) ◽  
pp. 481
Author(s):  
Liyabona Mpondo ◽  
Kingsley Ehi Ebomah ◽  
Anthony Ifeanyi Okoh
Keyword(s):  
South Africa ◽  
Surface Waters ◽  
Multidrug Resistant ◽  
Eastern Cape ◽  
Environmental Waters ◽  
Potential Health ◽  
Phenotypic Resistance ◽  
Listeria Species ◽  
Polymerase Chain ◽  
Identification And Characterization

The prevalence of bacteria with multidrug-resistance (MDR) is a significant threat to public health globally. Listeria spp. are naturally ubiquitous, with L. monocytogenes particularly being ranked as important foodborne disease-causing microorganisms. This study aimed to evaluate the incidence and determine the antimicrobial resistance (AMR) profiles of multidrug-resistant Listeria spp. (MDRL) isolated from different environmental samples (river and irrigation water) in the Sarah Baartman District Municipality (SBDM), Eastern Cape Province (ECP), South Africa. Molecular identification and characterization were carried out using polymerase chain reaction (PCR) and isolates that exhibited phenotypic resistance were further screened for relevant antimicrobial-resistant genes (ARGs). Findings revealed a total of 124 presumptive Listeria isolates; 69 were molecularly confirmed Listeria species. Out of the confirmed species, 41 isolates (59%) were classified as L. monocytogenes while 9 (13%) were classified as L. welshimeri. All Listeria spp. exhibited phenotypic resistance against ampicillin, penicillin, and trimethoprim-sulphamethoxazole and further screening revealed ARGs in the following proportions: sulI (71%), blaTEM (66%), tetA (63%), and blaCIT (33%). Results confirmed the occurrence of ARGs among Listeria inhabiting surface waters of ECP. The present study indicates that the river water samples collected from SBDM are highly contaminated with MDRL, hence, constituting a potential health risk.

Conservation of animal genetic resources: approaches and technologies for in situ and ex situ conservation

2008 ◽  
Vol 42 ◽  
pp. 71-85 ◽  
Author(s):  
J.A. Woolliams ◽  
O. Matika ◽  
J. Pattison
Keyword(s):  
Rural Communities ◽  
Genetic Resources ◽  
Large Scale ◽  
Production Systems ◽  
Regional Distribution ◽  
Livestock Production ◽  
Ex Situ ◽  
Animal Genetic Resources ◽  
The Face ◽  
Livestock Products

SummaryLivestock production faces major challenges through the coincidence of major drivers of change, some with conflicting directions. These are:1. An unprecedented global change in demands for traditional livestock products such as meat, milk and eggs.2. Large changes in the demographic and regional distribution of these demands.3. The need to reduce poverty in rural communities by providing sustainable livelihoods.4. The possible emergence of new agricultural outputs such as bio-fuels making a significant impact upon traditional production systems.5. A growing awareness of the need to reduce the environmental impact of livestock production.6. The uncertainty in the scale and impact of climate change. This paper explores these challenges from a scientific perspective in the face of the large-scale and selective erosion of our animal genetic resources, and concludes thai there is a stronger and more urgent need than ever before to secure the livestock genetic resources available to humankind through a comprehensive global conservation programme.

Comparison of Prevalence, Antimicrobial Resistance, and Occurrence of Multidrug-Resistant Salmonella in Antimicrobial-Free and Conventional Pig Production

2006 ◽  
Vol 69 (4) ◽  
pp. 743-748 ◽  
Author(s):  
WONDWOSSEN A. GEBREYES ◽  
SIDDHARTHA THAKUR ◽  
W. E. MORGAN MORROW
Keyword(s):  
Antimicrobial Resistance ◽  
Production Systems ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Swine Production ◽  
Significant Difference ◽  
Conventional Farms ◽  
Resistance Patterns ◽  
Conventional Systems ◽  
On Farm

Conventional swine production evolved to routinely use antimicrobials, and common occurrence of antimicrobial-resistant Salmonella has been reported. There is a paucity of information on the antimicrobial resistance of Salmonella in swine production in the absence of antimicrobial selective pressure. Therefore, we compared the prevalence and antimicrobial resistance of Salmonella isolated from antimicrobial-free and conventional production systems. A total of 889 pigs and 743 carcasses were sampled in the study. Salmonella prevalence was significantly higher among the antimicrobial-free systems (15.2%) than the conventional systems (4.2%) (odds ratio [OR] = 4.23; P &lt; 0.05). Antimicrobial resistance was detected against 10 of the 12 antimicrobials tested. The highest frequency of resistance was found against tetracycline (80%), followed by streptomycin (43.4%) and sulfamethoxazole (36%). Frequency of resistance to most classes of antimicrobials (except tetracycline) was significantly higher among conventional farms than antimicrobial-free farms, with ORs ranging from 2.84 for chloramphenicol to 23.22 for kanamycin at the on-farm level. A total of 28 antimicrobial resistance patterns were detected. A resistance pattern with streptomycin, sulfamethoxazole, and tetracycline (n = 130) was the most common multidrug resistance pattern. There was no significant difference in the proportion of isolates with this pattern between the conventional (19.5%) and the antimicrobial-free systems (18%) (OR = 1.8; P &gt; 0.05). A pentaresistance pattern with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline was strongly associated with antimicrobial-free groups (OR = 5.4; P = 0.01). While showing the higher likelihood of finding antimicrobial resistance among conventional herds, this study also implies that specific multidrug-resistant strains may occur on antimicrobial-free farms. A longitudinal study with a representative sample size is needed to reach more conclusive results of the associations detected in this study.

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