scholarly journals Plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli and Klebsiella pneumoniae isolated from market retail fruits in Guangzhou, China

2019 ◽  
Vol Volume 12 ◽  
pp. 385-389 ◽  
Author(s):  
Fan Yang ◽  
Cong Shen ◽  
Xiaobin Zheng ◽  
Yan Liu ◽  
Mohamed Abd El-Gawad El-Sayed Ahmed ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Gene ◽  
Colistin Resistance

Prevalence of the colistin resistance gene mcr-1 in colistin-resistant Escherichia coli and Klebsiella pneumoniae isolated from humans in Thailand

2018 ◽  
Vol 15 ◽  
pp. 32-35 ◽  
Author(s):  
Warawan Eiamphungporn ◽  
Sakda Yainoy ◽  
Chakornpat Jumderm ◽  
Rachanis Tan-arsuwongkul ◽  
Surapee Tiengrim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Gene ◽  
Colistin Resistance

scholarly journals Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella pneumoniae

2016 ◽  
Vol 16 (3) ◽  
pp. 285-286 ◽  
Author(s):  
Nicole Stoesser ◽  
Amy J Mathers ◽  
Catrin E Moore ◽  
Nicholas PJ Day ◽  
Derrick W Crook
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Resistance Gene ◽  
Colistin Resistance

Detection of chromosomal and plasmid-mediated mechanisms of colistin resistance in Escherichia coli and Klebsiella pneumoniae from Indian food samples

2019 ◽  
Vol 16 ◽  
pp. 48-52 ◽  
Author(s):  
Abdul Ghafur ◽  
Chaitra Shankar ◽  
Palani GnanaSoundari ◽  
Manigandan Venkatesan ◽  
Deepa Mani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Food Samples ◽  
Colistin Resistance ◽  
Indian Food

scholarly journals Comparison of the Superpolymyxin and ChromID Colistin R Screening Media for the Detection of Colistin-Resistant Enterobacteriaceae from Spiked Rectal Swabs

2018 ◽  
Vol 63 (1) ◽  
Author(s):  
Delphine Girlich ◽  
Thierry Naas ◽  
Laurent Dortet
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Confidence Interval ◽  
Enterobacter Cloacae ◽  
Bacterial Isolates ◽  
Colistin Resistance ◽  
Gram Negative ◽  
Content Type ◽  
Rectal Swabs ◽  
Stool Samples

ABSTRACT The dissemination of carbapenemase-producing Enterobacteriaceae (CPE) has led to the increased use of colistin, which has resulted in the emergence of colistin-resistant Enterobacteriaceae worldwide. One of the most threatening scenarios is the dissemination of colistin resistance in CPE, particularly the plasmid-encoded resistance element MCR. Thus, it has now become mandatory to possess reliable media to screen for colistin-resistant Gram-negative bacterial isolates, especially Enterobacteriaceae. In this study, we evaluated the performances of the Superpolymyxin medium (ELITechGroup) and the ChromID Colistin R medium (bioMérieux) to screen for colistin-resistant Enterobacteriaceae from spiked rectal swabs. Stool samples were spiked with a total of 94 enterobacterial isolates (Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Enterobacter cloacae), including 53 colistin-resistant isolates. ESwabs (Copan Diagnostics) were then inoculated with those spiked fecal suspensions, and culture proceeded as recommended by both manufacturers. The sensitivity of detection of colistin-resistant Enterobacteriaceae was 86.8% (95% confidence interval [95% CI] = 74.0% to 94.0%) using both the Superpolymyxin medium and the ChromID Colistin R plates. Surprisingly, the isolates that were not detected were not the same for both media. The specificities were high for both media, at 97.9% (95% CI = 87.3% to 99.9%) for the Superpolymyxin medium and 100% (95% CI = 90.4% to 100%) for the ChromID Colistin R medium. Both commercially available media, ChromID Colistin R and Superpolymyxin, provide useful tools to screen for colistin-resistant Enterobacteriaceae from patient samples (rectal swabs) regardless of the level and mechanism of colistin resistance.

First report of Escherichia coli carrying the mobile colistin resistance gene mcr-1 in Turkey

2018 ◽  
Vol 15 ◽  
pp. 169-170 ◽  
Author(s):  
Cemil Kurekci ◽  
Muhsin Aydin ◽  
Ozkan Ufuk Nalbantoglu ◽  
Aycan Gundogdu
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Colistin Resistance ◽  
First Report

scholarly journals Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Bijaya Muktan ◽  
Upendra Thapa Shrestha ◽  
Binod Dhungel ◽  
Bagish Chandra Mishra ◽  
Nabaraj Shrestha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Colistin Resistance ◽  
Clinical Specimens ◽  
E Coli ◽  
Resistant Genes ◽  
Rectal Swabs

Abstract Background Plasmid-mediated resistance to the last-resort drugs: carbapenems and colistin is an emerging public health threat. The studies on the prevalence and co-expression of resistant genes among livestock and human pathogens are rare in Nepal. This is the first study in Nepal exploring the prevalence and co-existence of colistin resistance gene, mcr-1 along with carbapenemase resistance gene, OXA-48 in Escherichia coli isolated from poultry and clinical specimens. Methods A total of 240 rectal swabs from chickens of five different poultry farms of Kathmandu valley and 705 mid-stream urine samples from human subjects attending Kantipur Hospital, Kathmandu were collected between August, 2018 and March, 2019. Rectal swabs and urine specimens were cultured. E. coli isolated from the specimens were screened for antimicrobial susceptibility testing (AST) using disk diffusion method’. Minimum inhibitory concentration (MIC) of colistin was determined by agar dilution method using 0.5 µg/ml to 32 µg/ml. The E. coli isolates were first screened for mcr-1 followed by screening for OXA-48 genes using conventional Polymerase chain reaction (PCR). Results Of the total samples analyzed, E. coli was isolated from 31.7% (76/240) of poultry and 7.9% (56/705) of clinical specimens. In AST, 80% (61/76) of E. coli from poultry and 79% (44/56) from clinical specimens were MDR. The phenotypic prevalence of colistin resistance in poultry specimens were 31.6% (24/76) and clinical specimens were 21.4% (12/56). In PCR assay, 27.6% (21/76) of poultry and 19.6% (11/56) of clinical isolates had colistin resistant mcr-1 gene. MICs value of E. coli isolates ranged from 4 to 32 (µg/ml) in both clinical and poultry isolates. Prevalence of co-existing carbapenem resistance gene, OXA-48, among colistin resistant mcr-1 positive isolates was 38% (8/21) in poultry specimens and 18.2% (2/11) in clinical specimens. Conclusions The high prevalence of colistin and carbapenem resistant genes, and their co-existence in plasmid DNA of E. coli isolates in this study suggests the possible spread to other animal, human and environmental pathogens. Molecular methods in addition to the conventional diagnostics in laboratories can help in early diagnosis, effective management and control of their potential transmission.

scholarly journals Occurrence of the mobile colistin resistance gene mcr-3 in Escherichia coli from household pigs in rural areas

2018 ◽  
Vol 73 (6) ◽  
pp. 1721-1723 ◽  
Author(s):  
Jiyun Li ◽  
Anette Hulth ◽  
Lennart E Nilsson ◽  
Stefan Börjesson ◽  
Baoli Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Rural Areas ◽  
Colistin Resistance
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