scholarly journals First molecular characterization of related cases of healthcare-associated infections involving multidrug-resistant Enterococcus faecium vanA in Algeria

2018 ◽  
Vol Volume 11 ◽  
pp. 1483-1490 ◽  
Author(s):  
Sonia Benammar ◽  
Alix Pantel ◽  
Fabien Aujoulat ◽  
Messaoud Benmehidi ◽  
René Courcol ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Multidrug Resistant ◽  
Healthcare Associated Infections ◽  
Healthcare Associated

scholarly journals Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock

2019 ◽  
Vol 98 (11) ◽  
pp. 5892-5899 ◽  
Author(s):  
Yeong Bin Kim ◽  
Kwang Won Seo ◽  
Jong Bo Shim ◽  
Se hyun Son ◽  
Eun Bi Noh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Parent Stock

scholarly journals Molecular Characterization of Ampicillin-Resistant Enterococcus faecium Isolates from Hospitalized Patients in Norway

2003 ◽  
Vol 41 (6) ◽  
pp. 2330-2336 ◽  
Author(s):  
R. Jureen ◽  
J. Top ◽  
S. C. Mohn ◽  
S. Harthug ◽  
N. Langeland ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecium ◽  
Hospitalized Patients

scholarly journals Molecular Characterization of Pathogenicity Locus (PaLoc) and tcdC Genetic Diversity Among tcdA+B+ Clostridioides Difficile Clinical Isolates in Tehran, Iran

2020 ◽  
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Disease Severity ◽  
Genetic Variants ◽  
Rflp Analysis ◽  
Clostridioides Difficile ◽  
Pcr Rflp ◽  
The Common ◽  
Healthcare Associated

Abstract Background: Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized the toxinotypes and the tcdC genotypes from a collection of Iranian clinical C. difficile tcdA+B+ isolates with known ribotypes (RTs).Methods: Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. Toxinotyping was carried out based on a PCR-RFLP analysis of a 19.6 kb region encompassing the PaLoc. Genetic diversity of the tcdC gene was determined by the sequencing of the gene.Results: Of the 50 C. difficile isolates investigated, five distinct toxinotypes were recognized. Toxinotypes 0 (33/50, 66%) and V (11/50, 22%) were the most frequently found. C. difficile isolates of the toxinotype 0 mostly belonged to RT 001 (12/33, 36.4%), whereas toxinotype V consisted of RT 126 (9/11, 81.8%). The tcdC sequencing showed six variants (35/50, 70%); tcdC-sc3 (24%), tcdC-A (22%), tcdC-sc9 (18%), tcdC-B (2%), tcdC-sc14 (2%), and tcdC-sc15 (2%). The remaining isolates were wild-types (15/50, 30%) in the tcdC gene.Conclusions: The present study demonstrates that the majority of clinical tcdA+B+ isolates of C. difficile frequently harbor tcdC genetic variants. We also found that the RT 001/ toxinotype 0 and the RT 126/ toxinotype V are the most common types among Iranian isolates. Further studies are needed to investigate the putative association of various tcdC genotypes with CDI severity and its recurrence.

scholarly journals Current Pattern and Clinico-Bacteriological Profile of Healthcare Associated Infections (HAI) in an ICU Setting: An Observational Study

2021 ◽  
Author(s):  
Mradul Kumar Daga ◽  
Govind Mawari ◽  
Saman Wasi ◽  
Naresh Kumar ◽  
Udbhav Sharma ◽  
...  
Keyword(s):  
Age Groups ◽  
Healthcare Facility ◽  
Central Line ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Healthcare Associated Infections ◽  
Acinetobacter Species ◽  
Gram Negative ◽  
Causative Agents ◽  
Healthcare Associated

Abstract Objective To understand the pattern and types of healthcare associated infections (HAI) at our healthcare facility, and to determine the common causative agents and their antibiotic susceptibility profile. Methods One hundred consecutive patients diagnosed with HAI were enrolled and monitored; the causative organisms isolated on culture were recorded and their sensitivity profile was generated. Results Of the 100 patients diagnosed with HAI (mean age ± SD being 42 ± 17 years), there were a total of 110 hospital acquired infections with 10 patients having two infections each. Out of 100 patients with HAI, 69 patients had ventilator associated pneumonia (VAP), 21 patients had catheter associated urinary tract infection (CAUTI) patients, and 20 patients had central line associated bloodstream infection (CLABSI). There were 10 patients with both VAP and CAUTI. All of the HAIs were device associated. A total of 76 pathogens were isolated on culture. No organism was isolated in 40 HAI. Majority (94.7%) of the organisms isolated from HAIs were gram-negative bacteria and all were multidrug resistant. Seventy-seven of the enrolled patients expired while 23 were discharged from the hospital Conclusions Our study demonstrated that HAIs occur in patients of all age groups; younger patients are not spared. Majority of the HAIs were caused by multidrug resistant gram-negative bacteria and were associated with high patient mortality. Acinetobacter species was the most common organism associated with HAI.

scholarly journals Increased Involvement of Klebsiellapneumoniae and Enterococcusfaecium in Healthcare-Associated Infections of Intensive Care Units in Taiwan

Healthcare ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1349
Author(s):  
Yu-Ren Lin ◽  
Yen-Yue Lin ◽  
Chia-Peng Yu ◽  
Ya-Sung Yang ◽  
Chun-Gu Cheng ◽  
...  
Keyword(s):  
Intensive Care ◽  
Infection Control ◽  
Intensive Care Units ◽  
Enterococcus Faecium ◽  
Healthcare Providers ◽  
Incidence Density ◽  
Healthcare Associated Infections ◽  
Chi Square ◽  
Control Protocols ◽  
Healthcare Associated

Background: Healthcare-associated infections (HAIs) cause increases in length of stay, mortality, and healthcare costs. A previous study conducted in Taiwan obtained similar results to those reported in Korea and Japan in 2015. Changes in microorganisms have been noted in recent years. Understanding the recent condition of HAIs in intensive care units (ICUs) can enable healthcare providers to develop effective infection control protocols to reduce HAIs. Methods: We used the Taiwan Nosocomial Infection Surveillance System to evaluate the incidence densities of HAIs, the proportions of causative pathogens, and the proportions of antimicrobial resistance (AMR). The Poisson regression model was constructed to incidence density, and the chi-square test was used to assess proportion. Results: The incidence density of HAIs decreased 5.7 to 5.4 per 1000 person-days. However, the proportions of Klebsiella pneumoniae and Enterococcus faecium significantly increased. In addition, the proportions of carbapenem-resistant K. pneumoniae and vancomycin-resistant Enterococcus faecium significantly increased over time. Conclusion: Analysis of the microorganisms involved in HAIs in ICUs showed elevated proportions of K. pneumoniae and E. faecium with AMR. Infection control protocols have been implemented for several years and require improvements regarding environmental cleanliness and medical staff prevention.

scholarly journals Isolation, Identification and Molecular Characterization of Nocardiopsaceae Producing Novel Antibiotics From Soil Samples in Bingol, Turkey

2020 ◽  
Vol 11 (2) ◽  
pp. 94
Author(s):  
Samer Abuzerr ◽  
Kate Zinszer ◽  
Syamand Ahmed Qadir ◽  
Ekrem Atalan ◽  
Halgord Ali M. Farag ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gene Sequence ◽  
Multidrug Resistant ◽  
Physicochemical Analysis ◽  
Soil Samples ◽  
Morphological Properties ◽  
16S Rdna Gene ◽  
Life Threatening ◽  
Immense Potential

There is growing interest in the antimicrobial discovery of life-threatening multidrug-resistant pathogens. The study was undertaken to isolate, identify, and characterize antibiotic-producing actinomycetaceae, particularly nocadisosaceae, from soil samples of Bingol, Turkey. Soil samples were collected from three different regions of Bingol, Turkey. The physicochemical analysis of the soil samples was immediately measured using standard methods. This was followed by isolation of Nocardiopsaceae, nutritional tests, chemotaxonomic analysis, and molecular characterization. The isolated organisms showed morphological properties consistent with the Nocardiopsaceae soil bacteria.  The 16s rDNA gene sequence indicated a similarity between the strains with 99.86% which was Nocardiopsaceae synnemata-formans. The BLAST hits had a significant e-value of 0.005. The results of the present study revealed that soil Nocardiopsaceae of Bingol appears to have immense potential as a source of antibacterial compounds.

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