scholarly journals Phosphorylation of the PDH complex precedes HIF-1-mediated effects and PDK1 upregulation during the first hours of hypoxic treatment in HCC cells

Hypoxia ◽  
2016 ◽  
Vol Volume 4 ◽  
pp. 135-145 ◽  
Author(s):  
Claude Haan ◽  
Geoffroy Walbrecq ◽  
Ines Kozar ◽  
Iris Behrmann ◽  
Andreas David Zimmer
Keyword(s):  
Mediated Effects ◽  
Hypoxic Treatment ◽  
Hcc Cells

scholarly journals Matrix stiffness-mediated effects on stemness characteristics occurring in HCC cells

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32221-32231 ◽  
Author(s):  
Yang You ◽  
Qiongdan Zheng ◽  
Yinying Dong ◽  
Xiaoying Xie ◽  
Yaohui Wang ◽  
...  
Keyword(s):  
Matrix Stiffness ◽  
Mediated Effects ◽  
Hcc Cells

scholarly journals Long noncoding RNA MACC1-AS1 promotes the stemness of hepatocellular carcinoma cells by antagonizing miR-145

2020 ◽  
Vol 48 (4) ◽  
pp. 030006052092041
Author(s):  
Yuling Guo ◽  
Jiuhong Zhong ◽  
Fang Wu ◽  
Zhengyu Zhan
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Carcinoma Cells ◽  
Luciferase Reporter ◽  
Spheroid Formation ◽  
Master Regulators ◽  
Real Time Quantitative Pcr ◽  
Mediated Effects ◽  
Hcc Cells

Objectives This work aimed to investigate the roles of long noncoding (lnc)RNA MACC1-AS1 (MACC1 antisense RNA 1) in progression of hepatocellular carcinoma (HCC). Methods Real-time quantitative PCR, western blot, spheroid formation, aldehyde dehydrogenase isoform 1 (ALDH1) activity analysis, luciferase reporter assay, and RNA pull-down analysis were used to examine MACC1-AS1–mediated effects on HCC cell stemness. Results MACC1-AS1 was highly expressed in HCC tissues and cells. MACC1-AS1 positively regulated the expression of stemness master regulators and inhibited spheroid-forming ability and ALDH1 activity. Furthermore, MACC1-AS1 promoted the stemness of HCC cells by antagonizing microRNA (miR)-145 activity. Overexpression of miR-145 also attenuated HCC cell stemness. Conclusions This work revealed a novel MACC1-AS1/miR-145 axis that regulates the stemness of HCC cells.

Protective Effect of Vitamin E on Immune Triggered, Granulocyte Mediated Endothelial Injury

1984 ◽  
Vol 51 (01) ◽  
pp. 089-092 ◽  
Author(s):  
M A Boogaerts ◽  
J Van de Broeck ◽  
H Deckmyn ◽  
C Roelant ◽  
J Vermylen ◽  
...  
Keyword(s):  
Vitamin E ◽  
Protective Effect ◽  
Umbilical Vein ◽  
Endothelial Damage ◽  
Endothelial Injury ◽  
Mediated Effects ◽  
Anti Oxidant ◽  
Endothelial Cell Cultures ◽  
Vitro Model

SummaryThe effect of alfa-tocopherol on the cell-cell interactions at the vessel wall were studied, using an in vitro model of human umbilical vein endothelial cell cultures (HUEC). Immune triggered granulocytes (PMN) will adhere to and damage HUEC and platelets enhance this PMN mediated endothelial injury. When HUEC are cultured in the presence of vitamin E, 51Cr-leakage induced by complement stimulated PMN is significantly decreased and the enhanced cytotoxicity by platelets is completely abolished (p <0.001).The inhibition of PMN induced endothelial injury is directly correlated to a diminished adherence of PMN to vitamin E- cultured HUEC (p <0.001), which may be mediated by an increase of both basal and stimulated endogenous prostacyclin (PGI2) from alfa-tocopherol-treated HUEC (p <0.025). The vitamin E-effect is abolished by incubation of HUEC with the irreversible cyclo-oxygenase inhibitor, acetylsalicylic acid, but the addition of exogenous PGI2 could not reproduce the vitamin E-mediated effects.We conclude that vitamin E exerts a protective effect on immune triggered endothelial damage, partly by increasing the endogenous anti-oxidant potential, partly by modulating intrinsic endothelial prostaglandin production. The failure to reproduce vitamin E-protection by exogenously added PGI2 may suggest additional, not yet elucidated vitamin E-effects on endothelial metabolism.

Cannabinoid Cb1 receptor-mediated effects on white and brown adipocytes

2006 ◽  
Vol 114 (S 1) ◽  
Author(s):  
N Perwitz ◽  
B Meier ◽  
M Drenckhan ◽  
M Fasshauer ◽  
J Klein
Keyword(s):  
Cb1 Receptor ◽  
Cannabinoid Cb1 Receptor ◽  
Brown Adipocytes ◽  
Mediated Effects
Sign in / Sign up

Export Citation Format

Share Document