scholarly journals Comprehensive comparison of MetAP2 tissue and cellular expression pattern in lean and obese rodents

2018 ◽  
Vol Volume 11 ◽  
pp. 565-577 ◽  
Author(s):  
Jing Han ◽  
Yang Tang ◽  
Mingjian Lu ◽  
Haiqing Hua
Keyword(s):  
Expression Pattern ◽  
Cellular Expression ◽  
Comprehensive Comparison

Localization of the cellular expression pattern of cdc25NEF and ras in the juvenile rat brain

1993 ◽  
Vol 19 (4) ◽  
pp. 339-344 ◽  
Author(s):  
Wen Wei ◽  
Steven S. Schreiber ◽  
Michel Baudry ◽  
Georges Tocco ◽  
Daniel Broek
Keyword(s):  
Rat Brain ◽  
Expression Pattern ◽  
Cellular Expression

Analysis of the cellular expression pattern of β-CGRP in α-CGRP-deficient mice

2004 ◽  
Vol 476 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Burkhard Schütz ◽  
Daniela Mauer ◽  
Anne-Marie Salmon ◽  
Jean-Pierre Changeux ◽  
Andreas Zimmer
Keyword(s):  
Expression Pattern ◽  
Cellular Expression ◽  
Deficient Mice

scholarly journals Wnt Signaling Alteration in the Spinal Cord of Amyotrophic Lateral Sclerosis Transgenic Mice: Special Focus on Frizzled-5 Cellular Expression Pattern

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0155867 ◽  
Author(s):  
Carlos González-Fernández ◽  
Renzo Mancuso ◽  
Jaume del Valle ◽  
Xavier Navarro ◽  
Francisco Javier Rodríguez
Keyword(s):  
Spinal Cord ◽  
Amyotrophic Lateral Sclerosis ◽  
Transgenic Mice ◽  
Wnt Signaling ◽  
Expression Pattern ◽  
Special Focus ◽  
Cellular Expression ◽  
Lateral Sclerosis

scholarly journals Comparative analysis of cellular expression pattern of schizophrenia risk genes in human versus mouse cortex

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hai-Long Zhang ◽  
Jia-Wen Long ◽  
Wei Han ◽  
Jiuzhou Wang ◽  
Weichen Song ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Neuronal Cells ◽  
Gabaergic Neurons ◽  
Differentially Expressed ◽  
Risk Genes ◽  
Glutamatergic Neurons ◽  
Cellular Expression ◽  
Mouse Cortex ◽  
V1 Cortex ◽  
Human And Mouse

Abstract Background Schizophrenia is a common psychiatric disease with high hereditary. The identification of schizophrenia risk genes (SRG) has shed light on its pathophysiological mechanisms. Mouse genetic models have been widely used to study the function of SRG in the brain with a cell type specific fashion. However, whether the cellular expression pattern of SRG is conserved between human and mouse brain is not thoroughly studied. Results We analyzed the single-cell transcription of 180 SRG from human and mouse primary visual cortex (V1). We compared the percentage of glutamatergic, GABAergic and non-neuronal cells that express each SRG between mouse and human V1 cortex. Thirty percent (54/180) of SRG had significantly different expression rate in glutamatergic neurons between mouse and human V1 cortex. By contrast, only 5.6% (10/180) of SRG showed significantly different expression in GABAergic neurons, which is similar with the ratio of SRG (15/180) with species difference in total cell populations. Strikingly, the percentage of non-neuronal cells expressing all SRG are indistinguishable between human and mouse V1 cortex. We further analyzed the biological significance of differentially expressed SRG by gene ontology. The species-different SRG in glutamatergic neurons are highly expressed in dendrite and axon. They are enriched in the biological process of response to stimulus. However, the differentially expressed SRG in GABAergic neurons are enriched in the regulation of organelle organization. Conclusion GABAergic neurons are more conserved in the expression of SRG than glutamatergic neurons while the non-neuronal cells show the species conservation for the expression of all SRG. It should be cautious to use mouse models to study those SRG which show different cellular expression pattern between human and mouse cortex.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

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