scholarly journals The cytochrome P450 isoenzyme and some new opportunities for the prediction of negative drug interaction in vivo

2018 ◽  
Vol Volume 12 ◽  
pp. 1147-1156 ◽  
Author(s):  
Dmitriy Sychev ◽  
Ghulam Md Ashraf ◽  
Andrey Svistunov ◽  
Maksim Maksimov ◽  
Vadim Tarasov ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drug Interaction ◽  
Cytochrome P450 Isoenzyme

In vitro and in vivo assessment of cytochrome P450-mediated herb–drug interaction of Ssang-hwa-tang

2013 ◽  
Vol 136 (2) ◽  
pp. 450-457 ◽  
Author(s):  
Sang Yoon Lee ◽  
Ji-Yoon Lee ◽  
Wonku Kang ◽  
Kwang-il Kwon ◽  
Soo Jin Oh ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Drug Interaction ◽  
In Vivo Assessment

Methadone Toxicity Due to Smoking Cessation—A Case Report on the Drug-Drug Interaction Involving Cytochrome P450 Isoenzyme IA2

2011 ◽  
Vol 45 (6) ◽  
pp. e34-e34 ◽  
Author(s):  
Joy Wahawisan ◽  
Srikanth Kolluru ◽  
Tu Nguyen ◽  
Christina Molina ◽  
Joseph Speake
Keyword(s):  
Smoking Cessation ◽  
Cytochrome P450 ◽  
Drug Interaction ◽  
Case Report ◽  
Cytochrome P450 Isoenzyme ◽  
Drug Drug Interaction

Evaluation of Nigerian Medicinal Plants Extract on Human P-glycoprotein and Cytochrome P450 Enzyme Induction: Implications for Herb-Drug Interaction

2021 ◽  
Vol 23 ◽  
Author(s):  
Ogochukwu Amaeze ◽  
Emily S. Marques ◽  
Wei Wei ◽  
Sarah Lazzaro ◽  
Nathaniel Johnson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Enzyme Activity ◽  
Cytochrome P450 ◽  
Drug Interaction ◽  
Medicinal Plants ◽  
Mrna Expression ◽  
Enzyme Induction ◽  
Methanol Extracts ◽  
P Glycoprotein

Background: Herbal medicine represents a significant component of disease prevention and therapy in most African countries. Herb-drug interactions (HDI) can arise from the co-administration of herbal and orthodox medicines. Objective: This study assessed the potential for HDI of V. amygdalina, O. gratissimum, M. oleifera, A. indica, and P. nitida extracts using in vitro assays. Little is known about these medicinal plants' potential for drug interaction despite their extensive use in Nigeria for several disease conditions. Method: The medicinal plant crude extracts were evaluated for Cytochrome P450 (CYP) enzyme induction using cryopreserved human hepatocytes. Enzyme activity was determined by quantifying probe substrate metabolism and metabolite formation using liquid chromatography-mass spectrometry/mass spectrometry. The extracts were evaluated for the potential to inhibit P-glycoprotein (P-gp) activity using human embryonic kidney membrane vesicles over-expressing human P-gp. The herbal extracts in vivo drug interaction potential was predicted based on the USFDA drug interaction guidance. Result: O. gratissimum and P. nitida methanol extracts induced CYP1A2 enzyme activity by greater than 3-fold. P. nitida methanol extracts showed over 2-fold induction of CYP1A2 mRNA expression. O. gratissimum methanol extract induced CYP2B6 mRNA expression over 2-fold. P. nitida and A. indica methanol extracts showed potent inhibition of P-gp activity (IC50: 3.8 and 5.4 µg/mL), respectively, while V. amygdalina and M. oleifera methanol extracts showed moderate P-gp inhibition (IC50: 12.1 and 37.2 µg/mL, respectively). Conclusion: Our studies suggested that the medicinal plants’ extracts can modulate CYP enzymes and P-gp activity with the potential to cause herb-drug interaction in vivo.

Pharmacogenetics of Clozapine: In Vivo Role of Cytochrome P450 Isoforms and P-Glycoprotein

2008 ◽  
Vol 41 (05) ◽  
Author(s):  
E Jaquenoud-Sirot ◽  
B Knezevic ◽  
G Perla Morena ◽  
P Baumann ◽  
CB Eap
Keyword(s):  
Cytochrome P450 ◽  
P Glycoprotein ◽  
Cytochrome P450 Isoforms

In vivo Phenotyping Methods: Cytochrome P450 Probes with Emphasis on the Cocktail Approach

2017 ◽  
Vol 23 (14) ◽  
Author(s):  
Guillermo Alberto Keller ◽  
María Laura Ferreirós Gago ◽  
Roberto Alejandro Diez ◽  
Guillermo Di Girolamo
Keyword(s):  
Cytochrome P450 ◽  
Cocktail Approach
Sign in / Sign up

Export Citation Format

Share Document