scholarly journals MiR-10a-5p: A Promising Biomarker for Early Diagnosis and Prognosis Evaluation of Bladder Cancer

2021 ◽  
Vol Volume 13 ◽  
pp. 7841-7850
Author(s):  
Lei Yang ◽  
Hong-Fang Sun ◽  
Lin-Qing Guo ◽  
Hai-Bing Cao
Keyword(s):  
Bladder Cancer ◽  
Early Diagnosis ◽  
Diagnosis And Prognosis ◽  
Prognosis Evaluation

scholarly journals Hsp74, a Potential Bladder Cancer Marker, Has Direct Interaction with Keratin 1

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ling Chen ◽  
YaRong Wang ◽  
Le Zhao ◽  
Wei Chen ◽  
Chunhui Dong ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Early Diagnosis ◽  
Western Blot ◽  
Cancer Progression ◽  
Molecular Basis ◽  
Direct Interaction ◽  
Molecular Target ◽  
Therapeutic Monitoring ◽  
Diagnosis And Prognosis ◽  
Keratin 1

Early diagnosis and prognosis monitoring are very important for the survival of patients with bladder cancer. To identify candidate biomarkers of bladder cancer, we used a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, and immunohistochemistry. Hsp74 was identified with high expression in bladder cancer. The cellular location of expression products of gene Hsp74 showed that they were distributed into cytoplasm and keratin 1 was found to be associated with Hsp74. The results provide a new idea to understand the molecular basis of bladder cancer progression and pinpoint new potential molecular target for early diagnosis and therapeutic monitoring of bladder cancer.

scholarly journals Value of serum sTREM-1,D-lactate and I-FABP in diagnosis and prognosis evaluation in liver cirrhotic patients with sepsis

2020 ◽  
Author(s):  
Danhong Yang ◽  
Qiang Zhang ◽  
Weiping Wang ◽  
Yicheng Huang ◽  
Hongying Pan ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
No Value ◽  
Sepsis Group ◽  
Negative Effects ◽  
Fatty Acid Binding ◽  
Diagnosis And Prognosis ◽  
Increasing Trend ◽  
Cirrhotic Patients ◽  
Early Diagnose ◽  
Prognosis Evaluation

Abstract BackgroundSepsis is can cause serious negative effects among patients with cirrhosis.Finding reliable biomarker in early diagnose and prognosis in septic patients with liver cirrhosis is very important.We assessed value of serumsoluble triggering receptor expressed on myeloid cells-1 ( sTREM-1),D-lactate and Intestinal fatty acid binding protein(I-FABP) in diagnosis and prognosis evaluation in liver cirrhotic patients with sepsis.MethodsA prospective observational study of 158 cirrhosis patients was conducted. A total of 158cirrhosis patients were enrolled, 83 of whom were diagnosed with sepsis and 75 patients without sepsis during hospitalization.We evaluated value of early diagnostic and prognosis of cells-1(sTREM-1),D-lactate and I-FABP in cirrhotic patients with sepsis.Receiver operating characteristic curves (ROCs) were used to assess the ability of tested biomarkers to diagnose and prognosis cirrhotic patients with sepsis.ResultsOur results showed the CRP，PCT，sTREM-1, D-lactate and I-FABP were significantly higher in the sepsis group than in the no-sepsis group.,but only levels of serum sTREM-1 showed increasing trend with the severity of the cirrhotic patients with sepsis. The cutoff value of sTREM-1was 123.62 pg/mL and showed 84.3% sensitivity and 92.0% specificity and high accuracy in the early diagnosis for cirrhotic patients with sepsis.The AUC of sTREM-1 was 0.959(95%CI: 0.934–0. 985) and higher than that of CRP,PCT,D-lactate and I-FABP( P <0.001, P =0.002, P <0.001, P <0.001 respectively).Total 31(37.35%) cirrhotic patients with sepsis cases died during 90-day follow-up.Only sTREM-1 level had significance between survivors and non-survivors(263.99±213.79 vs 672.24±191.44,P=0.031).The AUCs of sTREM-1 for predicting 90-day mortality was 0.904( 95% CI:0.836-.971 ) and higher than that of CRP,PCT,D-lactate (P=0.001,p=0.003, P <0.001 respectively) The AUCs of I-FABP is only 0.482 and had no value of predicting prognosis.ConclusionSerum sTREM-1 is a valuable new biomarker for early diagnosis and prognosis in cirrhosis with sepsis, proving more accurate than CRP,PCT, D - lactate and I - FABP.Serum D-lactate and I-FABP levels can identify early cirrhosis with sepsis, but they is little relationship with the prognosis of patients.

scholarly journals The New Biomarker for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Based on Public Database Mining

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Hao Ding ◽  
Xiao-Xing Xiong ◽  
Guan-Lan Fan ◽  
Yue-Xiong Yi ◽  
Yu-Rou Chen ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Early Diagnosis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cervical Squamous Cell Carcinoma ◽  
Public Database ◽  
Endocervical Adenocarcinoma ◽  
Cerna Network ◽  
Diagnosis And Prognosis ◽  
Prognosis Evaluation

To reconstruct the ceRNA biological network of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) and to select an appropriate mRNA as a biomarker that could be used for CESC early diagnosis and prognosis evaluation. We downloaded CESC data from the TCGA public database, and statistical analysis was conducted with the R software to find out differential expressed genes encoding for lncRNAs, miRNAs, and mRNAs. The differentially expressed mRNAs (DEmRNAs) screened in the ceRNA network were analyzed for survival to find the mRNAs with significantly linked to the survival prognosis. These mRNAs were searched in the Pathological Atlas to identify the final appropriate mRNAs. Differential expression analysis revealed 773 lncRNAs, 94 miRNAs, and 2466 mRNAs. Survival analysis of DEmRNAs in the ceRNA network indicated that ADGRF4, ANXA8L1, HCAR3, IRF6, and PDE2A (P<0.05) were negatively correlated with survival time. Verification of these six DEmRNAs in the Pathology Atlas indicated that PDE2A was a possible biomarker for CESC patients. PDE2A might be a biomarker for early diagnosis and prognosis evaluation of CESC patients, but due to the lack of available data, further studies may be needed for confirmation.

scholarly journals Screening the genome for HCC-specific CpG methylation signatures as biomarkers for diagnosis and prognosis evaluation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Rui-kun Zhang ◽  
Jia-lin Liu
Keyword(s):  
Cox Regression ◽  
Malignant Tumors ◽  
Gene Expression Omnibus ◽  
The Cancer Genome Atlas ◽  
Survival Prediction ◽  
Training Set ◽  
Cpg Sites ◽  
Diagnosis And Prognosis ◽  
Patient Prognosis ◽  
Prognosis Evaluation

Abstract Background Hepatocellular carcinoma (HCC) is one of the most common and invasive malignant tumors in the world. The change in DNA methylation is a key event in HCC. Methods Methylation datasets for HCC and 17 other types of cancer were downloaded from The Cancer Genome Atlas (TCGA). The CpG sites with large differences in methylation between tumor tissues and paracancerous tissues were identified. We used the HCC methylation dataset downloaded from the TCGA as the training set and removed the overlapping sites among all cancer datasets to ensure that only CpG sites specific to HCC remained. Logistic regression analysis was performed to select specific biomarkers that can be used to diagnose HCC, and two datasets—GSE157341 and GSE54503—downloaded from GEO as validation sets were used to validate our model. We also used a Cox regression model to select CpG sites related to patient prognosis. Results We identified 6 HCC-specific methylated CpG sites as biomarkers for HCC diagnosis. In the training set, the area under the receiver operating characteristic (ROC) curve (AUC) for the model containing all these sites was 0.971. The AUCs were 0.8802 and 0.9711 for the two validation sets from the GEO database. In addition, 3 other CpG sites were analyzed and used to create a risk scoring model for patient prognosis and survival prediction. Conclusions Through the analysis of HCC methylation datasets from the TCGA and Gene Expression Omnibus (GEO) databases, potential biomarkers for HCC diagnosis and prognosis evaluation were ascertained.

Intelligent diagnosis and analysis of brain lymphoma based on imaging features

2021 ◽  
pp. 1-11
Author(s):  
Yipu Mao ◽  
Muliang Jiang ◽  
Fanyu Zhao ◽  
Liling Long
Keyword(s):  
Imaging Techniques ◽  
Experimental Studies ◽  
Diagnostic Value ◽  
Imaging Features ◽  
Flow Properties ◽  
Brain Lymphoma ◽  
Diagnosis And Prognosis ◽  
Primary Brain Lymphoma ◽  
Set Up ◽  
Prognosis Evaluation

Currently, DSC has been extensively studied in the diagnosis, differential diagnosis and prognosis evaluation of brain lymphoma, but it has not obtained a uniform standard. By combining DSC imaging features, this study investigated the imaging features and diagnostic value of several types of tumors such as primary brain lymphoma. At the same time, this study obtained data from brain lymphoma patients by data collection and set up different groups to conduct experimental studies to explore the correlation between IVIM-MRI perfusion parameters and DSC perfusion parameters in brain lymphoma. Through experimental research, it can be seen that the combination of two perfusion imaging techniques can more fully reflect the blood flow properties of the lesion, which is beneficial to determine the nature of the lesion.

Protein biomarkers in breast cancer-derived extracellular vesicles for use in liquid biopsies

2021 ◽  
Author(s):  
Dan Li ◽  
Wenjia Lai ◽  
Di Fan ◽  
Qiaojun Fang
Keyword(s):  
Breast Cancer ◽  
Early Diagnosis ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Breast Cancer Diagnosis ◽  
Extracellular Vesicle ◽  
Detection Methods ◽  
Protein Markers ◽  
Liquid Biopsies ◽  
Diagnosis And Prognosis

Breast cancer is the most common malignant disease in women worldwide. Early diagnosis and treatment can greatly improve the management of breast cancer. Liquid biopsies are becoming convenient detection methods for diagnosing and monitoring breast cancer due to their non-invasiveness and ability to provide real-time feedback. A range of liquid biopsy markers, including circulating tumor proteins, circulating tumor cells, and circulating tumor nucleic acids, have been implemented for breast cancer diagnosis and prognosis, with each having its own advantages and limitations. Circulating extracellular vesicles are messengers of intercellular communication that are packed with information from mother cells and are found in a wide variety of bodily fluids; thus, they are emerging as ideal candidates for liquid biopsy biomarkers. In this review, we summarize extracellular vesicle protein markers that can be potentially used for the early diagnosis and prognosis of breast cancer or determining its specific subtypes.

Ratiometric fluorescence analysis for miR-141 detection with hairpin DNA-templated silver nanoclusters

2021 ◽  
Author(s):  
Meiqing Liu ◽  
Ren Shen ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Fluorescence Analysis ◽  
Silver Nanoclusters ◽  
Ratiometric Fluorescence ◽  
Hairpin Dna ◽  
Robust Detection ◽  
Diagnosis And Prognosis

MicroRNAs (miRNAs) are important biomarkers for diseases diagnosis and prognosis. Accurate and robust detection of miRNAs greatly facilitates the early diagnosis and progress assessment of certain cancers. The current miRNAs...

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