scholarly journals Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis

2019 ◽  
Vol Volume 11 ◽  
pp. 10719-10729
Author(s):  
Pihong Li ◽  
Qiaolin Wu ◽  
Yihan Sun ◽  
Xiaoyu Pan ◽  
Yifan Han ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Molecular Mechanism ◽  
Papillary Thyroid Cancer ◽  
In Silico ◽  
In Silico Analysis ◽  
Papillary Thyroid ◽  
Qrt Pcr ◽  
Silico Analysis

scholarly journals METTL14 promotes tumorigenesis by regulating lncRNA OIP5-AS1/miR-98/ADAMTS8 signaling in papillary thyroid cancer

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Xiaoping Zhang ◽  
Dan Li ◽  
Chengyou Jia ◽  
Haidong Cai ◽  
Zhongwei Lv ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Xenograft Model ◽  
Luciferase Reporter ◽  
Papillary Thyroid ◽  
Qrt Pcr ◽  
The Relationship

Abstract Background Papillary thyroid cancer (PTC) is the most common type of cancer of the endocrine system. Long noncoding RNAs (lncRNAs) are emerging as a novel class of gene expression regulators associated with tumorigenesis. Through preexisting databases available for differentially expressed lncRNAs in PTC, we uncovered that lncRNA OIP5-AS1 was significantly upregulated in PTC tissues. However, the function and the underlying mechanism of OIP5-AS1 in PTC are poorly understood. Methods Expression of lncRNA OIP5-AS1 and miR-98 in PTC tissue and cells were measured by quantitative real-time PCR (qRT-PCR). And expression of METTL14 and ADAMTS8 in PTC tissue and cells were measured by qRT-PCR and western blot. The biological functions of METTL14, OIP5-AS1, and ADAMTS8 were examined using MTT, colony formation, transwell, and wound healing assays in PTC cells. The relationship between METTL14 and OIP5-AS1 were evaluated using RNA immunoprecipitation (RIP) and RNA pull down assay. And the relationship between miR-98 and ADAMTS8 were examined by luciferase reporter assay. For in vivo experiments, a xenograft model was used to investigate the effects of OIP5-AS1 and ADAMTS8 in PTC. Results Functional validation revealed that OIP5-AS1 overexpression promotes PTC cell proliferation, migration/invasion in vitro and in vivo, while OIP5-AS1 knockdown shows an opposite effect. Mechanistically, OIP5-AS1 acts as a target of miR-98, which activates ADAMTS8. OIP5-AS1 promotes PTC cell progression through miR-98/ADAMTS8 and EGFR, MEK/ERK pathways. Furthermore, RIP and RNA pull down assays identified OIP5-AS1 as the downstream target of METTL14. Overexpression of METTL14 suppresses PTC cell proliferation and migration/invasion through inhibiting OIP5-AS1 expression and regulating EGFR, MEK/ERK pathways. Conclusions Collectively, our findings demonstrate that OIP5-AS1 is a METTL14-regulated lncRNA that plays an important role in PTC progression and offers new insights into the regulatory mechanisms underlying PTC development.

scholarly journals In silico analysis of the molecular mechanism of postmenopausal osteoporosis

2015 ◽  
Vol 12 (5) ◽  
pp. 6584-6590 ◽  
Author(s):  
YANQING LIU ◽  
YUEQIU WANG ◽  
NAILONG YANG ◽  
SUNING WU ◽  
YANHUA LV ◽  
...  
Keyword(s):  
Postmenopausal Osteoporosis ◽  
Molecular Mechanism ◽  
In Silico ◽  
In Silico Analysis ◽  
Silico Analysis

In Silico Analysis of RET Variants in Medullary Thyroid Cancer

2015 ◽  
Vol 152 (4) ◽  
pp. 650-654 ◽  
Author(s):  
Thomas E. Heineman ◽  
Rohan Joshi ◽  
Marc A. Cohen ◽  
William I. Kuhel ◽  
David I. Kutler
Keyword(s):  
Thyroid Cancer ◽  
In Silico ◽  
Medullary Thyroid Cancer ◽  
In Silico Analysis ◽  
Medullary Thyroid ◽  
Silico Analysis

miR-744/eNOS/NO axis: A novel target to halt triple negative breast cancer progression

2021 ◽  
pp. 1-9
Author(s):  
Farah Hady El Kilany ◽  
Rana Ahmed Youness ◽  
Reem Amr Assal ◽  
Mohamed Zakaria Gad
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
In Silico ◽  
In Silico Analysis ◽  
No Production ◽  
Cellular Viability ◽  
Griess Reagent ◽  
Normal Tissues ◽  
Qrt Pcr ◽  
Silico Analysis

BACKGROUND: Nitric oxide (NO) may have a dual role in cancer. At low concentrations, endogenous NO promotes tumor growth and proliferation. However, at very high concentrations, it mediates cancer cell apoptosis and inhibits cancer growth. High levels of NO have been observed in blood of breast cancer (BC) patients, which increases tumor blood flow and promotes angiogenesis. To date, the regulation of NO-synthesizing enzyme, eNOS, by miRNAs has not been adequately investigated in BC. Therefore, the main aim of this study is to unravel the possible regulation of eNOS by miRNAs in BC and to examine their influence on NO production and BC progression. METHODS: Expression profile of eNOS in Egyptian BC patients and MDA-MB-231 cell lines was investigated using qRT-PCR. In-silico analysis was performed to predict a putative upstream regulator of eNOS. miR-744-5p was selected and its expression was quantified in BC tissues using qRT-PCR. MDA-MB-231 cells were cultured and transfected with miR-744-5p using lipofection method. NO levels were determined using Griess Reagent. Cellular viability and colony-forming ability were assessed using MTT and colony-forming assays; respectively. RESULTS: eNOS and miR-744-5p were significantly up-regulated in BC tissues compared to paired normal tissues. In-silico analysis revealed that miR-744-5p putatively binds to eNOS transcript with high binding scores. Transfection of MDA-MB-231 cells with miR-744-5p mimics resulted in a significant up-regulation of eNOS and consequently NO levels. In addition, miR-744-5p transfection led to an increase in cellular viability and colony-forming ability of the MDA-MB-231. CONCLUSION: miR-744-5p acts as an upstream positive regulator of the NO synthesizing enzyme, eNOS which in turn elevates NO levels. Furthermore, miR-744-5p is a novel oncogenic miRNA in BC. Thus, targeting miR-744/eNOS/NO axis may act as a therapeutic tool in TNBC.

The gene expression of GPER1 is low in fresh samples of papillary thyroid carcinoma (PTC), and in silico analysis

2021 ◽  
pp. 111397
Author(s):  
Ana Paula Santin Bertoni ◽  
Patrícia de Araujo Manfroi ◽  
Joelson Tomedi ◽  
Beatriz Maria Assis-Brasil ◽  
Erika Laurini de Souza Meyer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
In Silico ◽  
In Silico Analysis ◽  
Papillary Thyroid ◽  
Silico Analysis

Polymorphisms of the Highly Expressed IL-6 Gene in the Papillary Thyroid Cancer Susceptibility Among Chinese

2019 ◽  
Vol 19 (6) ◽  
pp. 443-451 ◽  
Author(s):  
Honghui Li ◽  
Hao Dai ◽  
Huajing Li ◽  
Baiya Li ◽  
Yuan Shao
Keyword(s):  
Risk Factor ◽  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Cancer Susceptibility ◽  
Additive Model ◽  
In Silico Analysis ◽  
The Cancer Genome Atlas ◽  
Dominant Model ◽  
Nucleotide Polymorphisms ◽  
Papillary Thyroid

Background: Papillary thyroid cancer (PTC) is the cardinal histologic type of thyroid cancer, which is the most prevalent kind of endocrine malignancy. The expression of IL-6 is found higher in thyroid carcinoma (THCA) samples than paired normal tissues based on The Cancer Genome Atlas (TCGA) and Genotype-Tissue expression (GTEx) database. In this study, we aimed to investigate the association between interleukin-6 (IL-6) polymorphisms and the PTC risk. Methods: A case-control study was designed using the following data: 241 PTC patients and 463 healthy controls. Five single nucleotide polymorphisms (SNPs) in IL-6 were selected and genotyped using Agena MassARRAY technology. Results: Our results revealed that SNP rs1800796 was associated with an increased PTC risk in co-dominant model (p = 0.042) and dominant model (p = 0.027). Rs1524107 was also a risk factor for PTC susceptibility in co-dominant model (p = 0.003), dominant model (p = 0.002) and log-additive model (p = 0.044). Moreover, rs2066992 significantly increased the PTC risk in co-dominant model and dominant model (p = 0.011, p = 0.009, respectively). Additionally, rs2069837 variant elevated the PTC risk based on dominant model (p = 0.041). In silico analysis, GTEx results for rs1800796, rs1524107 and rs2066992 variants are known to be associated with IL-6 gene expression. Using HaploReg, we found rs1800796, rs1524107 and rs2066992 in LD with functional importance. Conclusion: Our study indicates that IL-6 variants may be a risk factor involved in the pathogenesis and development of PTC.

scholarly journals Molecular Mechanism Underlying Hesperetin-induced Apoptosis by in silico Analysis and in Prostate Cancer PC-3 Cells

2013 ◽  
Vol 14 (7) ◽  
pp. 4347-4352 ◽  
Author(s):  
Shanmugam Sambantham ◽  
Mahendran Radha ◽  
Arumugam Paramasivam ◽  
Balakrishnan Anandan ◽  
Ragunathan Malathi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Molecular Mechanism ◽  
In Silico ◽  
In Silico Analysis ◽  
Induced Apoptosis ◽  
Silico Analysis
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