scholarly journals LncRNA miR503HG Regulates the Drug Resistance of Recurrent Cervical Squamous Cell Carcinoma Cells by Regulating miR-155/Caspase-3

2020 ◽  
Vol Volume 12 ◽  
pp. 1579-1585
Author(s):  
Shanshan Zhao ◽  
Mingxin Yu ◽  
Lei Wang
Keyword(s):  
Drug Resistance ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Caspase 3 ◽  
Cervical Squamous Cell Carcinoma ◽  
Carcinoma Cells

scholarly journals DEPTOR promotes survival of cervical squamous cell carcinoma cells and its silencing induces apoptosis through downregulating PI3K/AKT and by up-regulating p38 MAP kinase

Oncotarget ◽  
2016 ◽  
Vol 7 (17) ◽  
pp. 24154-24171 ◽  
Author(s):  
Kalanghad Puthankalam Srinivas ◽  
Remadevi Viji ◽  
Vipin Mohan Dan ◽  
Indira Sukumaran Sajitha ◽  
Rajappan Prakash ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Map Kinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cervical Squamous Cell Carcinoma ◽  
P38 Map Kinase ◽  
Carcinoma Cells

scholarly journals The primary growth of laryngeal squamous cell carcinoma cells in vitro is effectively supported by paired cancer-associated fibroblasts alone

Tumor Biology ◽  
2017 ◽  
Vol 39 (5) ◽  
pp. 101042831770551 ◽  
Author(s):  
Mei Wang ◽  
Chunping Wu ◽  
Yu Guo ◽  
Xiaojuan Cao ◽  
Wenwei Zheng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Laryngeal Cancer ◽  
Squamous Cell ◽  
Caspase 3 ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Carcinoma Cells ◽  
Cancer Associated Fibroblasts ◽  
Protein Levels

Most primarily cultured laryngeal squamous cell carcinoma cells are difficult to propagate in vitro and have a low survival rate. However, in our previous work to establish a laryngeal squamous cell carcinoma cell line, we found that laryngeal cancer-associated fibroblasts appeared to strongly inhibit the apoptosis of primarily cultured laryngeal squamous cell carcinoma cells in vitro. In this study, we investigated whether paired laryngeal cancer-associated fibroblasts alone can effectively support the growth of primarily cultured laryngeal squamous cell carcinoma cells in vitro. In all, 29 laryngeal squamous cell carcinoma specimens were collected and primarily cultured. The laryngeal squamous cell carcinoma cells were separated from cancer-associated fibroblasts by differential trypsinization and continuously subcultured. Morphological changes of the cultured laryngeal squamous cell carcinoma cells were observed. Immunocytofluorescence was used to authenticate the identity of the cancer-associated fibroblasts and laryngeal squamous cell carcinoma cells. Flow cytometry was used to quantify the proportion of apoptotic cells. Western blot was used to detect the protein levels of caspase-3. Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells. AMD3100 (a chemokine (C-X-C motif) receptor 4 antagonist) and an anti–chemokine (C-X-C motif) ligand 7 antibody were used to block the tumor-supporting capacity of cancer-associated fibroblasts. Significant apoptotic changes were detected in the morphology of laryngeal squamous cell carcinoma cells detached from cancer-associated fibroblasts. The percentage of apoptotic laryngeal squamous cell carcinoma cells and the protein levels of caspase-3 increased gradually in subsequent subcultures. In contrast, no significant differences in the proliferation capacity of laryngeal squamous cell carcinoma cells cocultured with cancer-associated fibroblasts were detected during subculturing. High level of chemokine (C-X-C motif) ligand 12 was detected in the culture supernatant of cancer-associated fibroblasts. The tumor-supporting effect of cancer-associated fibroblasts was significantly inhibited by AMD3100. Our findings demonstrate that the paired laryngeal cancer-associated fibroblasts alone are sufficient to support the primary growth of laryngeal squamous cell carcinoma cells in vitro and that the chemokine (C-X-C motif) ligand 12/chemokine (C-X-C motif) receptor 4 axis is one of the major contributors.

scholarly journals Overexpression of fibronectin confers cell adhesion-mediated drug resistance (CAM-DR) against 5-FU in oral squamous cell carcinoma cells

2014 ◽  
Vol 44 (4) ◽  
pp. 1376-1384 ◽  
Author(s):  
YOSHIHIRO NAKAGAWA ◽  
HIDEKI NAKAYAMA ◽  
MASASHI NAGATA ◽  
RYOJI YOSHIDA ◽  
KENTA KAWAHARA ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Squamous Cell Carcinoma ◽  
Cell Adhesion ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Carcinoma Cells
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