scholarly journals Recalibrating SARS-CoV-2 Antigen Rapid Lateral Flow Test Relative Sensitivity from Validation Studies to Absolute Sensitivity for Indicating Individuals Shedding Transmissible Virus

2021 ◽  
Vol Volume 13 ◽  
pp. 935-940
Author(s):  
Irene Petersen ◽  
Alexander Crozier ◽  
Iain Buchan ◽  
Michael J Mina ◽  
Jonathan W Bartlett
Keyword(s):  
Validation Studies ◽  
Relative Sensitivity ◽  
Lateral Flow ◽  
Absolute Sensitivity ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Recalibrating SARS-CoV-2 antigen rapid lateral flow test relative sensitivity from validation studies to absolute sensitivity for detecting individuals with live virus

2021 ◽  
Author(s):  
Irene Petersen ◽  
Alexander Crozier ◽  
Iain Buchan ◽  
Michael J Mina ◽  
Jonathan W Bartlett
Keyword(s):  
Validation Studies ◽  
Genetic Material ◽  
Relative Sensitivity ◽  
Lateral Flow ◽  
Relative Performance ◽  
Viral Kinetics ◽  
Live Virus ◽  
Predictive Values ◽  
Absolute Sensitivity ◽  
Performance Statistics

Testing for SARS-CoV-2 internationally has focused on COVID-19 diagnosis among symptomatic individuals using reverse transcriptase polymerase chain reaction (PCR) tests. Recently, however, SARS-CoV-2 antigen rapid lateral flow tests (LFT) have been rolled out in several countries for testing asymptomatic individuals in public health programmes. Validation studies for LFT have been largely cross-sectional, reporting sensitivity, specificity and predictive values of LFT relative to PCR. However, because PCR detects genetic material left behind for a long period when the individual is no longer infectious, these statistics can under-represent sensitivity of LFT for detecting infectious individuals, especially when sampling asymptomatic populations. LFTs (intended to detect individuals with live virus) validated against PCR (intended to diagnose infection) are not reporting against a gold standard of equivalent measurements. Instead, these validation studies have reported relative performance statistics that need recalibrating to the purpose for which LFT is being used. We present an approach to this recalibration. We derive a formula for recalibrating relative performance statistics from LFT vs PCR validation studies to give likely absolute sensitivity of LFT for detecting individuals with live virus. We show the differences between widely reported apparent sensitivities of LFT and its absolute sensitivity as a test of presence of live virus. After accounting for within-individual viral kinetics and epidemic dynamics within asymptomatic populations we show that a highly performant test of live virus should show a LFT-to-PCR relative sensitivity of less than 50% in conventional validation studies, which after re-calibration would be an absolute sensitivity of more than 80%. Further studies are needed to ascertain the absolute sensitivity of LFT as a test of infectiousness in COVID-19 responses. These studies should include sampling for viral cultures and longitudinal series of LFT and PCR, ideally in cohorts sampled from both contacts of cases and the general population.

Rapid and visual detection of benzothiostrobin residue in strawberry using quantum dot-based lateral flow test strip

2019 ◽  
Vol 283 ◽  
pp. 222-229 ◽  
Author(s):  
JiaKai Wu ◽  
JingWei Ma ◽  
Hong Wang ◽  
DongMei Qin ◽  
Li An ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Visual Detection ◽  
Test Strip ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Lateral flow test for visual detection of silver(I) based on cytosine-Ag(I)-cytosine interaction in C-rich oligonucleotides

2017 ◽  
Vol 184 (11) ◽  
pp. 4243-4250 ◽  
Author(s):  
Zebin Guo ◽  
Yafeng Zheng ◽  
Hui Xu ◽  
Baodong Zheng ◽  
Wanwei Qiu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

Time-Dependent Decay of Detectable Antibodies Against SARS-CoV-2: A Comparison of ELISA with Two Batches of a Lateral-Flow Test

2020 ◽  
Author(s):  
Mariangela Freitas Silveira ◽  
Marilia Mesenburg ◽  
Odir Antonio Dellagostin ◽  
Natasha R. Oliveira ◽  
Mara Andrade Colares Maia ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Time Dependent ◽  
Flow Test ◽  
Lateral Flow Test
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