scholarly journals More Than Skin Deep – the Effects of Ultraviolet Radiation on Cathepsin K and Progerin Expression in Cultured Dermal Fibroblasts

2021 ◽  
Vol Volume 14 ◽  
pp. 1561-1568
Author(s):  
Igor Aleksander Bednarski ◽  
Magdalena Ciążyńska ◽  
Jacek Kabziński ◽  
Ireneusz Majsterek ◽  
Dorota Sobolewska-Sztychny ◽  
...  
Keyword(s):  
Ultraviolet Radiation ◽  
Cathepsin K ◽  
Dermal Fibroblasts

scholarly journals Fernblock Prevents Dermal Cell Damage Induced by Visible and Infrared A Radiation

2018 ◽  
Vol 19 (8) ◽  
pp. 2250 ◽  
Author(s):  
Alicia Zamarrón ◽  
Silvia Lorrio ◽  
Salvador González ◽  
Ángeles Juarranz
Keyword(s):  
Cell Damage ◽  
Cathepsin K ◽  
Aging Effect ◽  
Dermal Fibroblasts ◽  
Skin Damage ◽  
Protective Capacity ◽  
Dermal Cell ◽  
Extracellular Matrix Components ◽  
Fibrillin 1 ◽  
Polypodium Leucotomos

Sun overexposure leads to higher risk of photoaging and skin cancer. The contribution of infrared (IR) and visible light (VIS) radiation is currently being taken into account in their pathogenesis. Erythema, hyperpigmentation, genotoxicity or the increase of matrix metalloproteinases (MMPs) expression are some of the effects induced by these types of radiation. Extracts of various botanicals endowed with antioxidant activity are emerging as new photoprotective compounds. A natural extract from Polypodium leucotomos (Fernblock®, FB) has antioxidant and photoprotective properties and exhibits a strong anti-aging effect. In this study, we evaluated the protective capacity of FB against the detrimental effects of infrared A (IRA) and VIS radiation in human dermal fibroblasts. We analyzed the effects of FB on the morphology, viability, cell cycle and expression of extracellular matrix components of fibroblasts subjected to VIS and IRA. Our results indicate that FB prevents cell damage caused by VIS and IRA. Moreover, it reduces the increase in MMP-1 and cathepsin K expression induced by both VIS and IRA radiation, and curbs alterations in fibrillin 1, fibrillin 2 and elastin expression. All these findings support FB as a feasible approach to prevent or treat skin damage caused by IRA or VIS exposure.

scholarly journals Fibroblast-like cells change gene expression of bone remodelling markers in transwell cultures

2020 ◽  
Vol 25 (1) ◽  
Author(s):  
Eliza S. Hartmann ◽  
Sabine Schluessel ◽  
Miriam I. Köhler ◽  
Felicitas Beck ◽  
Julia I. Redeker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Remodeling ◽  
Mrna Level ◽  
Three Dimensional ◽  
Cathepsin K ◽  
Culture Conditions ◽  
Dermal Fibroblasts ◽  
Monolayer Cultures ◽  
Bone Remodeling Markers

Abstract Introduction Periprosthetic fibroblast-like cells (PPFs) play an important role in aseptic loosening of arthroplasties. Various studies have examined PPF behavior in monolayer culture systems. However, the periprosthetic tissue is a three-dimensional (3D) mesh, which allows the cells to interact in a multidirectional way. The expression of bone remodeling markers of fibroblast-like cells in a multilayer environment changes significantly versus monolayer cultures without the addition of particles or cytokine stimulation. Gene expression of bone remodeling markers was therefore compared in fibroblast-like cells from different origins and dermal fibroblasts under transwell culture conditions versus monolayer cultures. Methods PPFs from periprosthetic tissues (n = 12), osteoarthritic (OA) synovial fibroblast-like cells (SFs) (n = 6), and dermal fibroblasts (DFs) were cultured in monolayer (density 5.5 × 103/cm2) or multilayer cultures (density 8.5 × 105/cm2) for 10 or 21 days. Cultures were examined via histology, TRAP staining, immunohistochemistry (anti-S100a4), and quantitative real-time PCR. Results Fibroblast-like cells (PPFs/SFs) and dermal fibroblasts significantly increased the expression of RANKL and significantly decreased the expression of ALP, COL1A1, and OPG in multilayer cultures. PPFs and SFs in multilayer cultures further showed a higher expression of cathepsin K, MMP-13, and TNF-α. In multilayer PPF cultures, the mRNA level of TRAP was also found to be significantly increased. Conclusion The multilayer cultures are able to induce significant expression changes in fibroblast-like cells depending on the nature of cellular origin without the addition of any further stimulus. This system might be a useful tool to get more in vivo like results regarding fibroblast-like cell cultures.

scholarly journals Ultraviolet A-Induced Cathepsin K Expression Is Mediated via MAPK/AP-1 Pathway in Human Dermal Fibroblasts

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102732 ◽  
Author(s):  
Qingfang Xu ◽  
Wei Hou ◽  
Yue Zheng ◽  
Chen Liu ◽  
Zijian Gong ◽  
...  
Keyword(s):  
Cathepsin K ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A

Effect of Ultraviolet Radiation on the Expression of Drug Transporters in In Vitro Skin Models

2020 ◽  
Author(s):  
Manon Barthe ◽  
Jean-Paul Thénot ◽  
Hanan Osman-Ponchet
Keyword(s):  
Ultraviolet Radiation ◽  
Uv Radiation ◽  
Drug Transporters ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Slc Transporters ◽  
Skin Cancers ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts

The majority of skin cancers are caused by over exposure to ultraviolet (UV) radiation. The effects of UV radiation on the expression of drug transporters expressed in human skin has never been studied. In this the effects of UVA and UVB irradiation on the expression of ATP-binding cassette (ABC) transporters and Solute carrier (SLC) transporters was evaluated in normal human epidermal keratinocytes (NHEK) and normal human dermal fibroblasts (NHDF) in primary culture. First experiments were intended to validate the inflammatory reaction in response to stimulation by lipopolysaccharide (LPS) in NHEK, NHDF and 3D-reconstructed human epidermis (3D-RHE) model. LPS treatment has shown to increase the expression of IL-8 and TNF-alpha in all three in vitro models. Expression of the most expressed ABC and SLC transporters was then measured in NHEK and NHDF after UVA (30 J/m²) and UVB (40 mJ/m²) irradiation. The most striking result was a significant 29-fold increase of the expression of SLCO4A1 in normal human dermal fibroblasts. In summary, this study shows for the first time a significant regulation of the expression of SLCO4A1 in human dermal fibroblasts induced by UVA irradiation. This finding is of particular interest as most of skin cancers are caused by over exposure to ultraviolet radiation and need to be considered in pharmacokinetic evaluation of topical drugs.

scholarly journals Ultraviolet Radiation Increases Tropoelastin Accumulation by a Post-Transcriptional Mechanism in Dermal Fibroblasts

1995 ◽  
Vol 105 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Elaine Schwartz ◽  
Erika Feinberg ◽  
Mark Lebwohl ◽  
Thomas J. Mariani ◽  
Charles D. Boyd
Keyword(s):  
Ultraviolet Radiation ◽  
Dermal Fibroblasts

Impact of Ultraviolet Radiation on Expression of Transforming Growth Factor β, Smad2, Metalloproteinases-1, -3, -8, -9, Cathepsin K and Progerin

2018 ◽  
Vol 94 (2) ◽  
pp. 362-369 ◽  
Author(s):  
Magdalena Ciążyńska ◽  
Igor A. Bednarski ◽  
Karolina Wódz ◽  
Joanna Narbutt ◽  
Michał Sobjanek ◽  
...  
Keyword(s):  
Growth Factor ◽  
Ultraviolet Radiation ◽  
Transforming Growth Factor ◽  
Cathepsin K ◽  
Transforming Growth Factor Β

Applications of Photoelectron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 506-507
Author(s):  
William J. Baxter
Keyword(s):  
Electron Microscopy ◽  
Thermal Decomposition ◽  
Chemical Composition ◽  
Ultraviolet Radiation ◽  
Thin Layer ◽  
Edge Effects ◽  
Electron Optics ◽  
Surface Layers ◽  
Crystalline Orientation ◽  
Fluorescent Screen

In this form of electron microscopy, photoelectrons emitted from a metal by ultraviolet radiation are accelerated and imaged onto a fluorescent screen by conventional electron optics. image contrast is determined by spatial variations in the intensity of the photoemission. The dominant source of contrast is due to changes in the photoelectric work function, between surfaces of different crystalline orientation, or different chemical composition. Topographical variations produce a relatively weak contrast due to shadowing and edge effects.Since the photoelectrons originate from the surface layers (e.g. ∼5-10 nm for metals), photoelectron microscopy is surface sensitive. Thus to see the microstructure of a metal the thin layer (∼3 nm) of surface oxide must be removed, either by ion bombardment or by thermal decomposition in the vacuum of the microscope.

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