scholarly journals Methods for extracting genomic DNA from whole blood samples: current perspectives

2014 ◽  
pp. 1 ◽  
Author(s):  
Lyn Griffiths ◽  
Diego Chacon-Cortes
Keyword(s):  
Whole Blood ◽  
Genomic Dna ◽  
Blood Samples ◽  
Whole Blood Samples

scholarly journals Comparison of commercial DNA extraction kits for the detection of bacterial genomic DNA from whole-blood samples using a broad-range PCR

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 4) ◽  
pp. P13
Author(s):  
B Krulova ◽  
E Nemcova ◽  
B Zaloudikova ◽  
P Nemec ◽  
T Freiberger
Keyword(s):  
Dna Extraction ◽  
Whole Blood ◽  
Genomic Dna ◽  
Blood Samples ◽  
Whole Blood Samples

scholarly journals An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 79-84 ◽  
Author(s):  
David J Clark ◽  
Catherine M Moore ◽  
Marc Flanagan ◽  
Katrien Van Bocxlaer ◽  
Evangelia-Theophano Piperaki ◽  
...  
Keyword(s):  
Whole Blood ◽  
Genomic Dna ◽  
Leishmania Donovani ◽  
Small Volume ◽  
Small Sample ◽  
Blood Samples ◽  
Novel Technology ◽  
Spin Column ◽  
Whole Blood Samples ◽  
Pathogen Dna

The aim of this study was to assess pathogen DNA extraction with a new spin column-based method (DNA-XT). DNA from either whole-blood samples spiked with Plasmodium falciparum or  Leishmania donovani amastigote culture was extracted with DNA-XT and compared with that produced by a commercial extraction kit (DNeasy®). Eluates from large and small sample volumes were assessed by PCR and spectroscopy. Using a small volume (5 μl) of blood, the DNA-XT and DNeasy methods produced eluates with similar DNA concentrations (0.63 vs 1.06 ng/μl, respectively). The DNA-XT method produced DNA with lower PCR inhibition than DNeasy. The new technique was also twice as fast and required fewer plastics and manipulations but had reduced total recovered DNA compared with DNeasy.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

Sign in / Sign up

Export Citation Format

Share Document