scholarly journals Tools for the analysis and characterization of therapeutic protein species

Biosimilars ◽  
2016 ◽  
pp. 17 ◽  
Author(s):  
Hartmut Schluter ◽  
Marceline Fuh ◽  
Pascal Steffen
Keyword(s):  
Therapeutic Protein ◽  
Protein Species

Identification and characterization of heat shock protein 27 protein species in human myocardial two-dimensional electrophoresis patterns

1997 ◽  
Vol 18 (15) ◽  
pp. 2823-2831 ◽  
Author(s):  
Christian Scheler ◽  
Eva-Christina Müller ◽  
Joachim Stahl ◽  
Ursula Müller-Werdan ◽  
Johann Salnikow ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 27 ◽  
Two Dimensional ◽  
Protein Species ◽  
Two Dimensional Electrophoresis ◽  
Identification And Characterization ◽  
Dimensional Electrophoresis

Purification and Characterization of the Sand Crab (Ovalipes bipustulatus) Coagulogen (Fibrinogen)

1981 ◽  
Vol 45 (01) ◽  
pp. 077-081 ◽  
Author(s):  
F Madaras ◽  
M Y Chew ◽  
J D Parkin
Keyword(s):  
Human Factor ◽  
Major Protein ◽  
Protein Species ◽  
Thiol Groups ◽  
Purification And Characterization ◽  
Panulirus Interruptus ◽  
Cell Homogenate ◽  
Lysine Residues ◽  
Human Thrombin

SummaryFrom the coagulocytes (amoebocytes) coagulogen (fibrinogen) was isolated, and purified on Sephacryl S-200.The cell homogenate contained one major protein species with a minimum molecular weight of 70,000. This protein clotted in the presence of human thrombin, human factor XIII and Ca++.The coagulogen contained no free thiol groups, however these were detectable in the reduced protein. Using the cyanoethylation procedure, it was estimated that one coagulogen molecule contained two lysine residues which participated in the cross-linking reaction. The total amino add composition of the crab coagulogen and coagulin (fibrin) was estimated and compared with the amino acid composition of the Limulus polyphemus, lobster (Panulirus interruptus) and porcine fibrinogen.

Characterization of a cathepsin D protease from CHO cell-free medium and mitigation of its impact on the stability of a recombinant therapeutic protein

2017 ◽  
Vol 34 (1) ◽  
pp. 120-129 ◽  
Author(s):  
Amareth Lim ◽  
Brandon L. Doyle ◽  
Gerard M. Kelly ◽  
Angelia M. Reed-Bogan ◽  
Lawrence H. Breen ◽  
...  
Keyword(s):  
Cathepsin D ◽  
Therapeutic Protein ◽  
Free Medium ◽  
Cho Cell ◽  
Recombinant Therapeutic Protein ◽  
The Stability

Adaptation of proteomic techniques for the identification and characterization of protein species from murine heart

Amino Acids ◽  
2010 ◽  
Vol 41 (2) ◽  
pp. 401-414 ◽  
Author(s):  
Karima Schwab ◽  
Boris Neumann ◽  
Christian Scheler ◽  
Peter R. Jungblut ◽  
Franz Theuring
Keyword(s):  
Protein Species ◽  
Identification And Characterization ◽  
Proteomic Techniques

scholarly journals Establishment and characterization of new human cell lines for recombinant therapeutic protein production

2015 ◽  
Vol 9 (Suppl 9) ◽  
pp. P3
Author(s):  
Yuichi Inoue ◽  
Akira Iwamoto ◽  
Aiko Inoue ◽  
Hiroharu Kawahara
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Protein Production ◽  
Therapeutic Protein ◽  
Human Cell Lines ◽  
Recombinant Therapeutic Protein
Sign in / Sign up

Export Citation Format

Share Document