scholarly journals Quantitative immunohistochemistry using an antibody-fused bioluminescent protein

BioTechniques ◽  
2020 ◽  
Vol 69 (4) ◽  
pp. 302-306
Author(s):  
Ke-Yong Wang ◽  
Chun Wu ◽  
Shohei Shimajiri ◽  
Toshiteru Enomoto ◽  
Hidehiro Kubota ◽  
...  
Keyword(s):  
Detection Method ◽  
Imaging System ◽  
Light Emitting Diode ◽  
Protein Microarray ◽  
Photon Number ◽  
Light Signal ◽  
Quantitative Detection ◽  
Target Antigen ◽  
Light Emitting ◽  
Bioluminescent Protein

We established a quantitative detection method for immunohistochemistry based on a reference standard light-emitting diode, protein microarray and antibody-fused bioluminescent protein. In this procedure, we calibrated the bioluminescence imaging system and prepared the calibration curve between antigen and antibody-fused bioluminescent protein using a protein microarray. Then we converted the detecting light signal to antigen count via absolute photon number in the bioluminescent images; there was a resulting threefold difference in the target antigen number between normal and cancerous tissues. Our technique can easily compare immunohistological images and evaluate tumor progression in quantitative pathological diagnosis.

scholarly journals Light-Emitting Diode Based Photoacoustic Imaging System

2020 ◽  
Author(s):  
Ebrahim Najafzadeh ◽  
Parastoo Farnia ◽  
Alireza Ahmadian ◽  
Hossein Ghadiri
Keyword(s):  
Imaging System ◽  
Photoacoustic Imaging ◽  
Light Emitting Diode ◽  
Imaging Modality ◽  
Optical Excitation ◽  
Poly Vinyl Alcohol ◽  
Ultrasound Images ◽  
Light Emitting ◽  
Non Invasive ◽  
Wide Range

Purpose: A Photoacoustic Imaging (PAI) as a non-invasive hybrid imaging modality has the potential to be used in a wide range of pre-clinical and clinical applications. There are different optical excitation sources that affect the performance of PAI systems. Our goal is proving the capability of the Light-Emitting Diode (LED) based PAI system for imaging of objects in different depths. Materials and Methods: In this study the Full Width of Half Maximum (FWHM) and Contrast to Noise Ratio (CNR) of LED-based PAI system is evaluated using agar, and Poly-Vinyl Alcohol Cryogel (PVA-C) phantoms. Results: The results show that axial and lateral FWHM of the photoacoustic image in agar phantom 1%, are 0.59 and 1.16 mm, respectively. It is capable of distinguishing objects about 250 µm. Furthermore, one of the main improvements of photoacoustic images is achieved by proposed LED-based system that is a 26% higher CNR versus the ultrasound images. Conclusion: Therefore, the provided technical characteristics in this study have made designed LED-based PAI system as a suitable tool for preclinical and clinical imaging.

scholarly journals LightCue: An Innovative Far-Red Light Emitter for Locally Modifying the Spectral Cue in Outdoor Conditions with Global Consequences on Plant Architecture

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2483
Author(s):  
Alain Fortineau ◽  
Didier Combes ◽  
Céline Richard-Molard ◽  
Ela Frak ◽  
Alexandra Jullien
Keyword(s):  
Plant Architecture ◽  
Light Emitting Diode ◽  
Red Light ◽  
Inhibitory Effect ◽  
Photosynthetic Photon Flux Density ◽  
High Irradiance ◽  
Light Signal ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Emitting

Plasticity of plant architecture is a promising lever to increase crop resilience to biotic and abiotic damage. Among the main drivers of its regulation are the spectral signals which occur via photomorphogenesis processes. In particular, branching, one of the yield components, is responsive to photosynthetic photon flux density (PPFD) and to red to far-red ratio (R:FR), both signals whose effects are tricky to decorrelate in the field. Here, we developed a device consisting of far-red light emitting diode (LED) rings. It can reduce the R:FR ratio to 0.14 in the vicinity of an organ without changing the PPFD in outdoor high irradiance fluctuating conditions, which is a breakthrough as LEDs have been mostly used in non-fluctuant controlled conditions at low irradiance over short periods of time. Applied at the base of rapeseed stems during the whole bolting-reproductive phase, LightCue induced an expected significant inhibitory effect on two basal targeted axillary buds and a strong unexpected stimulatory effect on the overall plant aerial architecture. It increased shoot/root ratio while not modifying the carbon balance. LightCue therefore represents a promising device for progress in the understanding of light signal regulation in the field.

Wide-band suppression of photon-number fluctuations in a high-speed light-emitting diode driven by a constant-current source

1998 ◽  
Vol 72 (3) ◽  
pp. 284-286 ◽  
Author(s):  
M. Kobayashi ◽  
M. Kohno ◽  
Y. Kadoya ◽  
M. Yamanishi ◽  
J. Abe ◽  
...  
Keyword(s):  
High Speed ◽  
Light Emitting Diode ◽  
Photon Number ◽  
Current Source ◽  
Wide Band ◽  
Constant Current ◽  
Constant Current Source ◽  
Light Emitting
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