scholarly journals An inexpensive air stream temperature controller and its use to facilitate temperature-controlled behavior inDrosophila

BioTechniques ◽  
2019 ◽  
Vol 66 (3) ◽  
pp. 159-161
Author(s):  
Ryan Sangston ◽  
Jay Hirsh
2018 ◽  
Author(s):  
Ryan Sangston ◽  
Jay Hirsh

AbstractControlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adultDrosophilamelanogaster. Utilizing flies expressing the temperature inducible channel dTrpA1 in dopaminergic neurons, we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature modulated biological reagents.Method SummaryWe develop widely applicable and affordable solution to rapidly changing temperature within an enclosed chamber using commercially available components.


Author(s):  
Erick Kim ◽  
Kamjou Mansour ◽  
Gil Garteiz ◽  
Javeck Verdugo ◽  
Ryan Ross ◽  
...  

Abstract This paper presents the failure analysis on a 1.5m flex harness for a space flight instrument that exhibited two failure modes: global isolation resistances between all adjacent traces measured tens of milliohm and lower resistance on the order of 1 kiloohm was observed on several pins. It shows a novel method using a temperature controlled air stream while monitoring isolation resistance to identify a general area of interest of a low isolation resistance failure. The paper explains how isolation resistance measurements were taken and details the steps taken in both destructive and non-destructive analyses. In theory, infrared hotspot could have been completed along the length of the flex harness to locate the failure site. However, with a field of view of approximately 5 x 5 cm, this technique would have been time prohibitive.


2014 ◽  
Vol 29 (6) ◽  
pp. 1110-1118 ◽  
Author(s):  
Chuanhui Gu ◽  
William P. Anderson ◽  
Jeffrey D. Colby ◽  
Christopher L. Coffey

2006 ◽  
Vol 61 (17) ◽  
pp. 5654-5663 ◽  
Author(s):  
Morteza Khanahmadi ◽  
Reza Roostaazad ◽  
David A. Mitchell ◽  
Mokhtar Miranzadeh ◽  
Ramin Bozorgmehri ◽  
...  

1992 ◽  
Vol 2 (5) ◽  
pp. 503-510 ◽  
Author(s):  
F. Carmona ◽  
E. Valot ◽  
L. Servant ◽  
M. Ricci

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