scholarly journals Laser Microdissection and Laser Pressure Catapulting for the Generation of Chromosome-Specific Paint Probes

BioTechniques ◽  
1999 ◽  
Vol 27 (2) ◽  
pp. 362-367 ◽  
Author(s):  
L. Schermelleh ◽  
S. Thalhammer ◽  
W. Heckl ◽  
H. Pösl ◽  
T. Cremer ◽  
...  
Keyword(s):  
Laser Microdissection ◽  
Laser Pressure Catapulting

Deep proteomic characterization of fiber types of murine muscles by laser microdissection and mass spectrometry

2019 ◽  
Author(s):  
B Eggers ◽  
K Schork ◽  
R Schröder ◽  
CS Clemen ◽  
C Berwanger ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Microdissection ◽  
Fiber Types

scholarly journals Erratum: Velada et al. Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA. Biology 2021, 10, 209

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 718
Author(s):  
Isabel Velada ◽  
Esther Menéndez ◽  
Rita Teresa Teixeira ◽  
Hélia Cardoso ◽  
Augusto Peixe
Keyword(s):  
Laser Microdissection ◽  
High Quality ◽  
Stem Base ◽  
Rna Biology

The author wishes to make an erratum to the published version of the paper [...]

scholarly journals Sample preparation for laser-microdissection of soybean shoot apical meristem

2012 ◽  
Vol 3 (1) ◽  
pp. 3 ◽  
Author(s):  
Chui E. Wong ◽  
Mohan B. Singh ◽  
Prem L. Bhalla
Keyword(s):  
Sample Preparation ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Laser Microdissection ◽  
Specific Cell ◽  
Legume Crop ◽  
Continuous Formation ◽  
Molecular Events ◽  
Depth Analysis ◽  
Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

scholarly journals Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

10.3791/50231 ◽  
2013 ◽  
Author(s):  
Dorothée Sturm ◽  
Lorella Marselli ◽  
Florian Ehehalt ◽  
Daniela Richter ◽  
Marius Distler ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Laser Microdissection ◽  
Human Pancreatic Islets

Laser microdissection-based analysis of hypoxia- and thioredoxin-related genes in human stable carotid plaques

2009 ◽  
Vol 18 (5) ◽  
pp. 294-300 ◽  
Author(s):  
Nobuya Okami ◽  
Takakazu Kawamata ◽  
Gou Yamamoto ◽  
Yoshikazu Okada ◽  
Tomokatsu Hori ◽  
...  
Keyword(s):  
Laser Microdissection ◽  
Carotid Plaques
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