scholarly journals Detection of Alexandrium tamarensis by Rapid PCR Analysis

BioTechniques ◽  
1999 ◽  
Vol 26 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Sheean T. Haley ◽  
Jane F. Cavender ◽  
Thomas E. Murray
Keyword(s):  
Pcr Analysis ◽  
Rapid Pcr

scholarly journals Rapid PCR analysis of the St14 (DXS52) VNTR

1991 ◽  
Vol 19 (8) ◽  
pp. 1944-1944 ◽  
Author(s):  
B. Richards ◽  
R. Heilig ◽  
I. Oberlé ◽  
L. Storjohann ◽  
G.T. Horn
Keyword(s):  
Pcr Analysis ◽  
Rapid Pcr

Development of Battery-Powered, Portable Instrumentation for Rapid PCR Analysis

2002 ◽  
Author(s):  
Phillip Belgrader ◽  
Bill Benett ◽  
Dean Hadley ◽  
Ray Mariella Jr ◽  
M Allen Northrup ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Portable Instrumentation ◽  
Rapid Pcr

scholarly journals Microarrays for Public Health: Genomic Epidemiology of Tuberculosis

2002 ◽  
Vol 3 (4) ◽  
pp. 362-365 ◽  
Author(s):  
Jamila Shafi ◽  
Peter W. Andrew ◽  
Michael R. Barer
Keyword(s):  
Genomic Analysis ◽  
Pcr Analysis ◽  
Outbreak Strain ◽  
Outbreak Management ◽  
Genomic Epidemiology ◽  
School Based ◽  
Rapid Pcr ◽  
Initial Comparison ◽  
Microarray Studies ◽  
Public Health Genomic

In response to a large local school-based outbreak of tuberculosis, we have been evaluating the utility of microarray bacterial genomic analysis in outbreak management. After initial comparison of the isolate from the index case withMycobacterium tuberculosisH37Rv, it was possible to design robust PCRs directed towards strain-specific deletions. Rapid PCR analysis of isolates proved valuable in determining whether or not other isolates were compatible with the outbreak strain and further microarray studies revealed genetic markers that could be used to discriminate between locally circulating strains.We suggest that this approach forms the basis for developing rapid local genotyping schemes applicable toM. tuberculosisand that application to other pathogens warrants consideration.

scholarly journals Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies

2002 ◽  
Vol 68 (5) ◽  
pp. 2209-2213 ◽  
Author(s):  
Masaru Nomura ◽  
Miho Kobayashi ◽  
Takashi Okamoto
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Lactococcus Lactis ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Variants ◽  
Glutamate Decarboxylase ◽  
Fragment Length Polymorphism ◽  
Pcr Analysis ◽  
Rapid Pcr ◽  
Restriction Fragment Length

ABSTRACT A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of different L. lactis strains resulted in specific fragments. The length polymorphism of the PCR fragments allowed a clear distinction of the L. lactis subspecies. The amplified fragment length polymorphism with the primers and the restriction fragment length polymorphism of the amplified products agreed perfectly with the identification based on genotypic and phenotypic analyses, respectively. Isolates from cheese starters were investigated by this method, and amplified fragments of genetic variants were found to be approximately 40 bp shorter than the typical L. lactis subsp. cremoris fragments.

Characterization of Partial Gene Deletions in Type III von Willebrand Disease with Alloantibody Inhibitors

1994 ◽  
Vol 72 (02) ◽  
pp. 180-185 ◽  
Author(s):  
David J Mancuso ◽  
Elodee A Tuley ◽  
Ricardo Castillo ◽  
Norma de Bosch ◽  
Pler M Mannucci ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Von Willebrand Disease ◽  
Pcr Analysis ◽  
Gene Deletions ◽  
Factor Gene ◽  
Type Iii ◽  
Large Deletions ◽  
Von Willebrand ◽  
Willebrand Factor

Summaryvon Willebrand factor gene deletions were characterized in four patients with severe type III von Willebrand disease and alloantibodies to von Willebrand factor. A PCR-based strategy was used to characterize the boundaries of the deletions. Identical 30 kb von Willebrand factor gene deletions which include exons 33 through 38 were identified in two siblings of one family by this method. A small 5 base pair insertion (CCTGG) was sequenced at the deletion breakpoint. PCR analysis was used to detect the deletion in three generations of the family, including two family members who are heterozygous for the deletion. In a second family, two type III vWD patients, who are distant cousins, share an -56 kb deletion of exons 22 through 43. The identification and characterization of large vWF gene deletions in these type III vWD patients provides further support for the association between large deletions in both von Willebrand factor alleles and the development of inhibitory alloantibodies.

REP-PCR Analysis of Erwinia Genus Bacteria – Infectious Agents of Apple Trees Diseases in Ukraine

2019 ◽  
Vol 81 (6) ◽  
pp. 45-57
Author(s):  
L.A. Dankevych ◽  
◽  
F.V. Muchnyk ◽  
V.P. Patyka ◽  
◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Infectious Agents ◽  
Apple Trees

S-allele identification by PCR analysis in Lithuanian sweet cherries

Biologija ◽  
2008 ◽  
Vol 54 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Vidmantas Stanys ◽  
Rugilė Stanytė ◽  
Gražina Stanienė ◽  
Jurgita Vinskienė
Keyword(s):  
Pcr Analysis ◽  
Sweet Cherries ◽  
S Allele ◽  
Allele Identification

Cloning and Expression of Recombinant Human Insulin Gene in Pichia pastoris

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Pichia Pastoris ◽  
Human Insulin ◽  
Expression Vector ◽  
Expression System ◽  
Insulin Gene ◽  
Cloning And Expression ◽  
Pcr Analysis ◽  
Major Band ◽  
Human Insulin Gene ◽  
Pichia Pastoris Expression System

The main goal of the current study was cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand, by using two sets of specific primers contain EcoR1 and Notl restriction sites. The amplified preproinsulin gene fragment was double digested with EcoRI and Not 1 restriction enzymes, then inserted into pPIC9K expression vector. The new pPIC9K-hpi constructive expression vector was transformed by the heat-shock method into the E.coli DH5α competent cells. pPic9k –hpi, which was propagated in the positive transformant E. coli cells, was isolated from cells and then linearised by restriction enzyme SalI, then transformed into Pichia pastoris GS115 using electroporation method. Genomic DNA of His+ transformants cell was extracted and used as a template for PCR analysis. The results showed, that the pPic9k – hpi was successfully integrated into the P. pastoris genome, for selected His+ transformants clones on the anticipated band at 330 bp, which is corresponded to the theoretical molecular size of the human insulin gene. To follow the insulin expression in transformans, Tricine–SDS gel electrophoresis and Western blot analysis were conducted. The results showed a successful expression of recombinant protein was detected by the presence of a single major band with about (5.8 KDa) on the gel. These bands correspond well with the size of human insulin with the theoretical molecular weight (5.8 KDa).

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