scholarly journals Small-Scale Preparation of the Single-Copy Bacterial Artificial Chromosome Vector pBeloBAC11

BioTechniques ◽  
1998 ◽  
Vol 24 (4) ◽  
pp. 568-572 ◽  
Author(s):  
Daniel Tillett ◽  
Brett A. Neilan
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Single Copy ◽  
Small Scale ◽  
Bacterial Artificial Chromosome Vector ◽  
Scale Preparation

A Modular, Positive Selection Bacterial Artificial Chromosome Vector with Multiple Cloning Sites

Genomics ◽  
1999 ◽  
Vol 58 (3) ◽  
pp. 250-253 ◽  
Author(s):  
Eirik Frengen ◽  
Dieter Weichenhan ◽  
Baohui Zhao ◽  
Kazutoyo Osoegawa ◽  
Michel van Geel ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Positive Selection ◽  
Artificial Chromosome ◽  
Bacterial Artificial Chromosome Vector ◽  
Multiple Cloning Sites

scholarly journals Novel Bacterial Artificial Chromosome Vector pUvBBAC for Use in Studies of the Functional Genomics of Listeria spp.

2008 ◽  
Vol 74 (6) ◽  
pp. 1892-1901 ◽  
Author(s):  
Torsten Hain ◽  
Sonja Otten ◽  
Ulrich von Both ◽  
Som S. Chatterjee ◽  
Ulrike Technow ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Functional Genomics ◽  
Bac Library ◽  
Virulence Gene ◽  
Artificial Chromosome ◽  
Broad Host Range ◽  
Actin Assembly ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Bacterial Artificial Chromosome Vector

ABSTRACT Bacterial artificial chromosome (BAC) vectors are important tools for microbial genome research. We constructed a novel BAC vector, pUvBBAC, for replication in both gram-negative and gram-positive bacterial hosts. The pUvBBAC vector was used to generate a BAC library for the facultative intracellular pathogen Listeria monocytogenes EGD-e. The library had insert sizes ranging from 68 to 178 kb. We identified two recombinant BACs from the L. monocytogenes pUvBBAC library that each contained the entire virulence gene cluster (vgc) of L. monocytogenes and transferred them to a nonpathogenic Listeria innocua strain. Recombinant L. innocua strains harboring pUvBBAC+vgc1 and pUvBBAC+vgc2 produced the vgc-specific listeriolysin (LLO) and actin assembly protein ActA and represent the first reported cloning of the vgc locus in its entirety. The use of the novel broad-host-range BAC vector pUvBBAC extends the versatility of this technology and provides a powerful platform for detailed functional genomics of gram-positive bacteria as well as its use in explorative functional metagenomics.

Construction of a marsupial bacterial artificial chromosome library from the model Australian marsupial, the tammar wallaby (Macropus eugenii)

2005 ◽  
Vol 53 (6) ◽  
pp. 389 ◽  
Author(s):  
Natasha Sankovic ◽  
Wayne Bawden ◽  
John Martyn ◽  
Jennifer A. M. Graves ◽  
Kurt Zuelke
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Positional Cloning ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Single Copy ◽  
Tammar Wallaby ◽  
Average Insert Size ◽  
Insert Size ◽  
Macropus Eugenii ◽  
Australian Marsupial

With the accelerating recognition of the power of comparative genomics, there is now enormous interest in sequencing the genomes of a broad range of species. Marsupials diverged at an important evolutionary time. The model Australian marsupial, the tammar wallaby (Macropus eugenii), has long been a resource for biological and genetic studies of marsupials, and the availability of a bacterial artificial chromosome (BAC) library will be a valuable resource in these studies. A tammar wallaby BAC library was constructed using pRazorBAC vector. It contains 55 296 clones with an average insert size of 108 kb, representing 2.2 times coverage of the wallaby genome (based on an estimated 2.7 × 109 bp haploid genome size). The library was arrayed in 384-well plates, and spotted in duplicate onto nylon membranes. Screening these membranes has yielded clones containing 34 single-copy genes distributed over the genome, while it failed for only one gene. Each probe isolated 1–12 BAC clones and, to date, no chimeric clones have been found. This BAC library will constitute an invaluable resource for creating physical maps, positional cloning of genes and other sequences in the tammar wallaby, as well as comparative mapping studies in mammals.

scholarly journals Targeting CreERT2 expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis

2012 ◽  
Vol 21 (5) ◽  
pp. 1117-1123 ◽  
Author(s):  
Li Zhang ◽  
Boyu Zhang ◽  
Sang Jun Han ◽  
Amy N. Shore ◽  
Jeffrey M. Rosen ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Keratin 8

scholarly journals Revising the Chromosome-Specific Probes of White Hawk (Leucopternis albicollis)

Proceedings ◽  
2020 ◽  
Vol 76 (1) ◽  
pp. 1
Author(s):  
Ivanete Furo ◽  
Rafael Kretschmer ◽  
Jorge Pereira ◽  
Darren Griffin ◽  
Rebecca O’Connor ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Bird Of Prey ◽  
Inaccurate Data ◽  
Intrachromosomal Rearrangements

Leucopternis albicollis is a diurnal bird of prey with extensive karyotype reorganization. Chromosome-specific probes from this species have been used successfully to detect intrachromosomal rearrangements in different species of bird since 2010. However, some gaps were detected in this first set of probes. Here, we have obtained a new set of whole chromosome probes in order to improve the previous one; also, we have performed experiments using bacterial artificial chromosome (BAC) from chicken microchromosomes. Our results demonstrated that the microchromosomes were involved in fusion events. In addition, a new nomenclature has been proposed for the new set of probes and some inaccurate data were corrected.

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