scholarly journals Cosmid Vector for the Cloning and Study of Complex GC-Rich Genomes

BioTechniques ◽  
1997 ◽  
Vol 23 (5) ◽  
pp. 812-814
Author(s):  
Sirinart Ananvoranich ◽  
Claire Simard
Keyword(s):  
Cosmid Vector

scholarly journals Complete nucleotide sequence of the cosmid vector pWE15A

1992 ◽  
Vol 20 (14) ◽  
pp. 3786-3786 ◽  
Author(s):  
Donald Seto ◽  
Ben F. Koop ◽  
Jason Seto ◽  
Leroy Hood
Keyword(s):  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Cosmid Vector

Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector.

1987 ◽  
Vol 6 (2) ◽  
pp. 355-361 ◽  
Author(s):  
D. Kioussis ◽  
F. Wilson ◽  
C. Daniels ◽  
C. Leveton ◽  
J. Taverne ◽  
...  
Keyword(s):  
Tumour Necrosis Factor ◽  
Tumour Necrosis ◽  
Human Tumour ◽  
Factor Gene ◽  
Human Tumour Necrosis Factor ◽  
Cosmid Vector ◽  
Necrosis Factor

Cloning and expression of Xylella fastidiosa antigens in Escherichia coli and Erwinia stewartii

1989 ◽  
Vol 35 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul H. Goodwin
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Genomic Dna ◽  
Xylella Fastidiosa ◽  
Gene Bank ◽  
Cloning And Expression ◽  
Western Blots ◽  
E Coli ◽  
Cosmid Vector ◽  
Erwinia Stewartii

Xylella fastidiosa DNA, partially digested with Sau3A, was ligated into the cosmid vector, pUCD615. Approximately 4500 ampicillin-resistant Escherichia coli colonies were obtained. The frequency of complementation of leucine auxotrophy in transfected E. coli indicated that the cosmid gene bank was representative of X. fastidiosa genomic DNA. Colonies were lysed directly onto nitrocellulose membranes using a thermo-inducible λ lysogen and screened for expression of X. fastidiosa antigens. Approximately 16.5% of a random sample of clones were found to express X. fastidiosa antigens as determined by Western blots. These proteins comigrated with proteins of X. fastidiosa and ranged in molecular weight from 10 000 to 160 000. Conjugation of several of the plasmids into Erwinia stewartii resulted in expression of the similar molecular weight cloned proteins with similar levels of expression as in E. coli.Key words: Xylella fastidiosa, Pierce's disease, immunological clone screening, thermo-inducible lysogeny.

Construction and functional screening of a metagenomic library using a T7 RNA polymerase-based expression cosmid vector

2010 ◽  
Vol 38 (9) ◽  
pp. 1321-1328 ◽  
Author(s):  
François-Xavier Lussier ◽  
Olivier Chambenoit ◽  
Amélie Côté ◽  
Jean-François Hupé ◽  
François Denis ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Metagenomic Library ◽  
T7 Rna Polymerase ◽  
Functional Screening ◽  
Cosmid Vector
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