scholarly journals Simultaneous Expression of Multi-Subunit Proteins in Mammalian Cells Using a Convenient Set of Mammalian Cell Expression Vectors

BioTechniques ◽  
1997 ◽  
Vol 23 (3) ◽  
pp. 402-407 ◽  
Author(s):  
M.I. Cockett ◽  
R. Ochalski ◽  
K. Benwell ◽  
R. Franco ◽  
J. Wardwell-Swanson
Keyword(s):  
Mammalian Cell ◽  
Mammalian Cells ◽  
Expression Vectors ◽  
Mammalian Cell Expression ◽  
Cell Expression ◽  
Simultaneous Expression

Comparison of mammalian cell expression vectors with and without an EBV-replicon

1988 ◽  
Vol 103 (3-4) ◽  
pp. 157-166 ◽  
Author(s):  
A. Jalanko ◽  
A. Kallio ◽  
I. Ulmanen
Keyword(s):  
Mammalian Cell ◽  
Expression Vectors ◽  
Mammalian Cell Expression ◽  
Cell Expression

scholarly journals DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss

2021 ◽  
Vol 15 (5) ◽  
pp. e0009374
Author(s):  
Kit Man Chai ◽  
Tsai-Teng Tzeng ◽  
Kuan-Yin Shen ◽  
Hung-Chun Liao ◽  
Jhe-Jhih Lin ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Neutralizing Antibodies ◽  
Protective Immunity ◽  
Dna Vaccination ◽  
Spike Protein ◽  
Expression Vectors ◽  
Global Public Health ◽  
Protein Vaccine ◽  
Mammalian Cell Expression ◽  
Cell Expression

The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic acid-based vaccine is a novel option, beyond the traditional inactivated virus vaccine or recombinant protein vaccine. Here, we report a DNA vaccine containing the spike gene for delivery via electroporation. The spike genes of SARS-CoV and SARS-CoV-2 were codon optimized for mammalian cell expression and then cloned into mammalian cell expression vectors, called pSARS-S and pSARS2-S, respectively. Spike protein expression was confirmed by immunoblotting after transient expression in HEK293T cells. After immunization, sera were collected for antigen-specific antibody and neutralizing antibody titer analyses. We found that both pSARS-S and pSARS2-S immunization induced similar levels of antibodies against S2 of SARS-CoV-2. In contrast, only pSARS2-S immunization induced antibodies against the receptor-binding domain of SARS-CoV-2. We further found that pSARS2-S immunization, but not pSARS-S immunization, could induce very high titers of neutralizing antibodies against SARS-CoV-2. We further analyzed SARS-CoV-2 S protein-specific T cell responses and found that the immune responses were biased toward Th1. Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. These data suggest that DNA vaccination could be a promising approach for protecting against COVID-19.

scholarly journals High Sensitivity and Low-Cost Flavin luciferase (FLUX)-based Reporter Gene for Mammalian Cell Expression

2021 ◽  
Author(s):  
Jittima Phonbuppha ◽  
Ruchanok Tinikul ◽  
Yoshihiro Ohmiya ◽  
Pimchai Chaiyen
Keyword(s):  
Reporter Gene ◽  
Mammalian Cell ◽  
Mammalian Cells ◽  
Low Cost ◽  
High Sensitivity ◽  
Firefly Luciferase ◽  
Nf Kappa B ◽  
Mammalian Cell Expression ◽  
Reporter Assays ◽  
Cell Expression

Luciferase-based gene reporters generating bioluminescence signals are important tools for biomedical research. Amongst the luciferases, flavin-dependent enzymes use the most common, and thus most economical chemicals. However, their applications in mammalian cells are limited due to their low signals compared to other systems. Here, we constructed Flavin Luciferase for Mammalian Cell Expression (FLUX) by engineering luciferase from Vibrio campbellii (the most thermostable bacterial luciferase reported to date) and optimizing its expression and reporter assays in mammalian cells. We found that the FLUX reporter gene can be overexpressed in various cell lines and showed outstanding signal-to-background in HepG2 cells, significantly higher than that of firefly luciferase (Fluc). The combined use of FLUX/Fluc as target/control vectors gave the most stable signals, better than the standard set of Fluc(target)/Rluc(control). We demonstrated that FLUX can be used for testing inhibitors of the NF-kappa-B signaling pathway, validating FLUX applications for various assays in the future.

Mammalian cell expression of an active site mutant of Pseudomonas exotoxin disrupts LRP1 maturation

2008 ◽  
Vol 15 (4) ◽  
pp. 427-439 ◽  
Author(s):  
Diana V. Pastrana ◽  
Cheol H. Yun ◽  
Marian L. McKee ◽  
David J. FitzGerald
Keyword(s):  
Mammalian Cell ◽  
Active Site ◽  
Pseudomonas Exotoxin ◽  
Mammalian Cell Expression ◽  
Cell Expression

scholarly journals Analysis of the molecular event of ICAM‐1 interaction with LFA‐1 during leukocyte adhesion using a reconstituted mammalian cell expression model

2001 ◽  
Vol 5 (3) ◽  
pp. 253-262
Author(s):  
Weon‐Cheol Han ◽  
Kwon‐Seop Kim ◽  
Jae‐Seung Park ◽  
Sung‐Yeoun Hwang ◽  
Hyung‐Bae Moon ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Leukocyte Adhesion ◽  
Molecular Event ◽  
Mammalian Cell Expression ◽  
Expression Model ◽  
Cell Expression
Sign in / Sign up

Export Citation Format

Share Document