scholarly journals In Situ Trypan Blue Staining of Monolayer Cell Cultures for Permanent Fixation and Mounting

BioTechniques ◽  
1997 ◽  
Vol 22 (6) ◽  
pp. 1020-1024 ◽  
Author(s):  
Seth W. Perry ◽  
Leon G. Epstein ◽  
Harris A. Gelbard
Keyword(s):  
Cell Cultures ◽  
Trypan Blue ◽  
Blue Staining ◽  
Monolayer Cell ◽  
Trypan Blue Staining ◽  
Permanent Fixation

Trypan blue staining in vitreoretinal surgery

Ophthalmology ◽  
2004 ◽  
Vol 111 (8) ◽  
pp. 1622-1623 ◽  
Author(s):  
Gema Rebolleda ◽  
Francisco José Muñoz Negrete ◽  
Marta Suarez-Figueroa
Keyword(s):  
Trypan Blue ◽  
Vitreoretinal Surgery ◽  
Blue Staining ◽  
Trypan Blue Staining

Use of trypan blue staining to assess the quality of ovarian cryopreservation

2007 ◽  
Vol 87 (5) ◽  
pp. 1200-1207 ◽  
Author(s):  
Patricia Fauque ◽  
Anis Ben Amor ◽  
Christiane Joanne ◽  
Germain Agnani ◽  
Jean Luc Bresson ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Blue Staining ◽  
Trypan Blue Staining

scholarly journals Inadvertent Trypan Blue Staining of Posterior Capsule during Cataract Surgery Associated with “Argentinian Flag” Event

2016 ◽  
Vol 2016 ◽  
pp. 1-3
Author(s):  
Robert A. Prinzi ◽  
Neeti M. Alapati ◽  
Shawn S. Gappy ◽  
Jason S. Dilly
Keyword(s):  
Cataract Surgery ◽  
Trypan Blue ◽  
Rare Complication ◽  
Posterior Capsule ◽  
Blue Staining ◽  
Anterior Surface ◽  
Anterior Capsule ◽  
Trypan Blue Staining ◽  
First Case

Trypan blue is common in visualizing the anterior capsule during cataract surgery. Inadvertent staining of the posterior capsule during phacoemulsification is a rare complication and there are few reports in the literature. The proposed mechanism of posterior capsule staining in previous reports includes a compromised zonular apparatus or iris retractors facilitating the posterior flow of trypan blue. We report the first case of trypan blue staining of the posterior capsule associated with the “Argentinian flag” sign. In our case, the “Argentinian flag” allowed the trypan blue to seep between the posterior capsule and the lens, staining the anterior surface of the posterior capsule.

Finding a Novel Threadlike Structure on the Intra-abdominal Organ Surface of Small Pigs by Using In Vivo Trypan Blue Staining

2011 ◽  
pp. 63-69 ◽  
Author(s):  
Ayati M. Hossein ◽  
Tian Yu-Ying ◽  
Huang Tao ◽  
Zhang Yu-Qing ◽  
Che Yong-Zhe ◽  
...  
Keyword(s):  
Trypan Blue ◽  
Blue Staining ◽  
Abdominal Organ ◽  
Trypan Blue Staining

scholarly journals Trypan Blue - Adapting a Dye Used for Labelling Dead Cells to Visualize Pinocytosis in Viable Cells.

2021 ◽  
Vol 55 (S1) ◽  
pp. 171-184
Keyword(s):  
Cell Death ◽  
Ion Channels ◽  
Silver Nitrate ◽  
Trypan Blue ◽  
Blue Staining ◽  
Fluorescence Signal ◽  
Hypotonic Solution ◽  
Trypan Blue Staining ◽  
Cell Death Pathway ◽  
Viable Cells

BACKGROUND/AIMS: Trypan blue is routinely used in cell culture experiments to distinguish between dead cells, which are labelled by trypan blue, and viable cells, which are apparently free of any staining. The assumption that trypan blue labelling is restricted to dead cells derives from the observation that rupture of the plasma membrane correlates with intense trypan blue staining. However, decades ago, trypan blue has been used to trace fluid uptake by viable macrophage-like cells in animals. These studies contributed to the concept of the reticuloendothelial system in vertebrates. Trypan blue itself does not show a fluorescence signal, but trypan blue-labelled proteins do. Therefore, intracellular localization of trypan blue-labelled proteins could give a clue to the entrance pathway of the dye in viable cells. METHODS: We used fluorescence microscopy to visualize trypan blue positive structures and to evaluate whether the bactericide, silver, enhances cellular trypan blue uptake in the brain macrophage-like cell line, BV-2. The pattern of chromatin condensation, visualized by DAPI staining, was used to identify the cell death pathway. RESULTS: We observed that silver nitrate at elevated concentrations (≥ 10 µM) induced in most cells a necrotic cell death pathway. Necrotic cells, identified by pycnotic nuclei, showed an intense and homogenous trypan blue staining. Apoptotic cells, characterized by crescent-like nuclear chromatin condensations, were not labelled by trypan blue. At lower silver nitrate concentrations, most cells were viable, but they showed trypan blue labelling. Viable cells showed a cell-type specific distribution of heterochromatin and revealed a perinuclear accumulation of bright trypan blue-labelled vesicles and, occasionally, a faint homogenous trypan blue labelling of the cytoplasm and nucleus. Amiloride, which prevents macropinocytosis by blocking the Na+ / H+ exchange, suppressed perinuclear accumulation of dye-labelled vesicles. Swelling of cells in a hypotonic solution induced an intense intracellular accumulation of trypan blue. Cells exposed to a hypotonic solution in the presence of 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), which blocks volume-regulated ion channels, prevented labelling of the cytoplasm and nucleus but did not affect labelling of perinuclear vesicles. CONCLUSION: In viable cells trypan blue-labelled vesicles indicate trypan blue uptake by macropinocytosis and trypan blue-labelled cytosol could indicate a further entry pathway for the dye, like activated volume-regulated channels. Accordingly, fluorescence microscopic analysis of trypan blue-labelled cells allows not only a discrimination between necrotic and apoptotic cell death pathway but also a discrimination between the mode of trypan blue uptake in viable cells - via pinocytosis or via activated volume-regulated ion channels - in the same preparation at the single cell level.

Trypan blue staining method for quenching the autofluorescence of RPE cells for improving protein expression analysis

2011 ◽  
Vol 89 (s248) ◽  
pp. 0-0
Author(s):  
GK SRIVASTAVA ◽  
R REINOSO ◽  
AK SINGH ◽  
A CORELL ◽  
I FERNANDEZ‐BUENO1 ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Analysis ◽  
Trypan Blue ◽  
Staining Method ◽  
Blue Staining ◽  
Rpe Cells ◽  
Trypan Blue Staining

Plant Tissue Trypan Blue Staining During Phytopathogen Infection

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (24) ◽  
Author(s):  
Nuria Fernández-Bautista ◽  
José Domínguez-Núñez ◽  
M. Mar Moreno ◽  
Marta Berrocal-Lobo
Keyword(s):  
Plant Tissue ◽  
Trypan Blue ◽  
Blue Staining ◽  
Trypan Blue Staining
Sign in / Sign up

Export Citation Format

Share Document