scholarly journals Real-Time Assay of the Interaction of a GST Fusion Protein with a Protein Ligate Using Resonant Mirror Technique

BioTechniques ◽  
1997 ◽  
Vol 22 (2) ◽  
pp. 269-271 ◽  
Author(s):  
Ignacio Rubio ◽  
Philip Buckle ◽  
Hans Trutnau ◽  
Reinhard Wetzker
Keyword(s):  
Fusion Protein ◽  
Real Time ◽  
Gst Fusion Protein

Kinetics of interaction of HIV fusion protein (gp41) with lipid membranes studied by real-time AFM imaging

2010 ◽  
Vol 110 (6) ◽  
pp. 694-700 ◽  
Author(s):  
Arkady Bitler ◽  
Naama Lev ◽  
Yael Fridmann-Sirkis ◽  
Lior Blank ◽  
Sidney R. Cohen ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Real Time ◽  
Lipid Membranes ◽  
Afm Imaging ◽  
Protein Gp41 ◽  
Kinetics Of

Production of Recombinant Melittin by Auto-Induction in Escherichia coli

2013 ◽  
Vol 798-799 ◽  
pp. 1007-1012
Author(s):  
Wen He Zhu ◽  
Wei Zhang ◽  
Yan Li ◽  
Jun Jie Xu ◽  
Shi Jie Lv
Keyword(s):  
Fusion Protein ◽  
Large Scale ◽  
Expression Vector ◽  
Human Cancer ◽  
Expression System ◽  
Scale Production ◽  
Recognition Sequence ◽  
Large Scale Production ◽  
N Terminus ◽  
Gst Fusion Protein

Melittin is a novel peptide of biological activity isolated from bee venom. It has potential application value in medicine and agriculture. Here we encoded melittin gene with the EK recognition sequence in the N-terminus into expression vector pGEX-2T.The expressed fusion protein, which is about 29KDa, identified by Western Blot. To facilitate large-scale production of recombinant GST-fusion protein, we optimized different expression conditions to increase the overall production of the fusion protein. The production of the protein had increased about 10-fold when we used an auto-inducing medium. The GST fusion protein showed an equivalent activity with the natural melittin after digested by EK and can inhibited the proliferations of several human cancer lines. The expression system described in this study provides a feasible way for producing melittin in further studies.

scholarly journals Expression in Bacteria of the Gene Encoding the gp43 Antigen of Paracoccidioides brasiliensis: Immunological Reactivity of the Recombinant Fusion Proteins

2002 ◽  
Vol 9 (6) ◽  
pp. 1200-1204 ◽  
Author(s):  
Susana N. Diniz ◽  
Kátia C. Carvalho ◽  
Patrícia S. Cisalpino ◽  
José F. Silveira ◽  
Luiz R. Travassos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Fusion Protein ◽  
Fusion Proteins ◽  
Inclusion Bodies ◽  
Paracoccidioides Brasiliensis ◽  
Immunological Reactivity ◽  
Gene Encoding ◽  
Recombinant Fusion Proteins ◽  
Gst Fusion Protein

ABSTRACT gp43 is the major diagnostic antigen of Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis (PCM) in humans. In the present study, cDNA of the gp43 gene (PbGP43) was obtained by reverse transcriptase PCR, inserted into a pGEX vector in frame with the glutathione S-transferase (GST) gene, and expressed in Escherichia coli as inclusion bodies. Immunoblotting showed that all sera from patients with chronic pulmonary and acute lymphatic forms of PCM reacted with the recombinant fusion protein of the mature gp43 (381 amino acids). Reactivity with fusion proteins containing subfragments of the N-terminal, internal, or C-terminal regions occurred eventually, and the C-terminal region was the most antigenic. Lack of reactivity with the subfragments may be due to the conformational nature of the gp43 epitopes. Sera from patients with aspergillosis, candidiasis, and histoplasmosis did not react with the gp43-GST fusion protein. Our results suggest that recombinant gp43 corresponding to the processed antigen can be a useful tool in the diagnosis of PCM.

scholarly journals Isolation of Dihydroflavonol 4-Reductase cDNA Clones from Angelonia x angustifolia and Heterologous Expression as GST Fusion Protein in Escherichia coli

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107755 ◽  
Author(s):  
Christian Gosch ◽  
Karthik Mudigere Nagesh ◽  
Jana Thill ◽  
Silvija Miosic ◽  
Sylvia Plaschil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Heterologous Expression ◽  
Cdna Clones ◽  
Gst Fusion Protein

Glutathione (GSH)-decorated magnetic nanoparticles for binding glutathione-S-transferase (GST) fusion protein and manipulating live cells

2011 ◽  
Vol 2 (5) ◽  
pp. 945 ◽  
Author(s):  
Yue Pan ◽  
Marcus J. C. Long ◽  
Xinming Li ◽  
Junfeng Shi ◽  
Lizbeth Hedstrom ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Fusion Protein ◽  
Live Cells ◽  
Glutathione S Transferase ◽  
Gst Fusion Protein
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