scholarly journals Aptamer-dependent full-length cDNA synthesis by overlap extension PCR

BioTechniques ◽  
2004 ◽  
Vol 37 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Yasumasa Mitani ◽  
Takayuki Nakayama ◽  
Matthias Harbers ◽  
Yoshihide Hayashizaki
Keyword(s):  
Full Length ◽  
Cdna Synthesis ◽  
Full Length Cdna ◽  
Overlap Extension Pcr ◽  
Overlap Extension

High-efficiency cloning of full-length cDNA

1982 ◽  
Vol 2 (2) ◽  
pp. 161-170
Author(s):  
H Okayama ◽  
P Berg
Keyword(s):  
Plasmid Dna ◽  
High Efficiency ◽  
Full Length ◽  
Mrna Sequence ◽  
Beta Globin ◽  
Cdna Synthesis ◽  
Globin Mrna ◽  
Full Length Cdna ◽  
Coding Regions ◽  
Entire Nucleotide Sequence

A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per microgram of rabbit reticulocyte mRNA, about 10% contained a complete alpha- of beta-globin mRNA sequence, and at least 30 to 50%, but very likely more, contained the entire globin coding regions. We attribute the high efficiency of cloning full- or nearly full-length cDNA to (i) the fact that the plasmid DNA vector itself serves as the primer for first- and second-strand cDNA synthesis, (ii) the lack of any nuclease treatment of the products, and (iii) the fact that one of the steps in the procedure results in preferential cloning of recombinants with full-length cDNA's over those with truncated cDNA's.

scholarly journals Full-Length cDNA Synthesis for Long-Distance RT-PCR of Large mRNA Transcripts

BioTechniques ◽  
2003 ◽  
Vol 34 (4) ◽  
pp. 768-773 ◽  
Author(s):  
Phillip R. Hawkins ◽  
Pei Jin ◽  
Glenn K. Fu
Keyword(s):  
Full Length ◽  
Rt Pcr ◽  
Cdna Synthesis ◽  
Long Distance ◽  
Full Length Cdna ◽  
Mrna Transcripts

scholarly journals High-efficiency cloning of full-length cDNA.

1982 ◽  
Vol 2 (2) ◽  
pp. 161-170 ◽  
Author(s):  
H Okayama ◽  
P Berg
Keyword(s):  
Plasmid Dna ◽  
High Efficiency ◽  
Full Length ◽  
Mrna Sequence ◽  
Beta Globin ◽  
Cdna Synthesis ◽  
Globin Mrna ◽  
Full Length Cdna ◽  
Coding Regions ◽  
Entire Nucleotide Sequence

A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per microgram of rabbit reticulocyte mRNA, about 10% contained a complete alpha- of beta-globin mRNA sequence, and at least 30 to 50%, but very likely more, contained the entire globin coding regions. We attribute the high efficiency of cloning full- or nearly full-length cDNA to (i) the fact that the plasmid DNA vector itself serves as the primer for first- and second-strand cDNA synthesis, (ii) the lack of any nuclease treatment of the products, and (iii) the fact that one of the steps in the procedure results in preferential cloning of recombinants with full-length cDNA's over those with truncated cDNA's.

Establishment and Identification of a Normalized Full-Length cDNA Library of CCRI 36

2009 ◽  
Vol 35 (4) ◽  
pp. 602-607 ◽  
Author(s):  
Dong WU ◽  
Jun-Jie LIU ◽  
Shu-Xun YU ◽  
Shu-Li FAN ◽  
Mei-Zhen SONG
Keyword(s):  
Cdna Library ◽  
Full Length ◽  
Full Length Cdna

Full length cDNA cloning and tissue expression of PRLR in Cyprinus carpio var. jian

2013 ◽  
Vol 20 (4) ◽  
pp. 722-732
Author(s):  
Bing LI ◽  
Jie WANG ◽  
Chengfeng ZHANG ◽  
Jian ZHU
Keyword(s):  
Cdna Cloning ◽  
Cyprinus Carpio ◽  
Tissue Expression ◽  
Full Length ◽  
Full Length Cdna

scholarly journals UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast

2020 ◽  
Vol 20 (4) ◽  
pp. 523-536
Author(s):  
Mohamad Al kadi ◽  
Nicolas Jung ◽  
Shingo Ito ◽  
Shoichiro Kameoka ◽  
Takashi Hishida ◽  
...  
Keyword(s):  
Full Length ◽  
Cdna Sequencing ◽  
Full Length Cdna ◽  
Novel Transcripts ◽  
Automated Pipeline
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