scholarly journals High-Yield Production of Recombinant Antibody Fragments in HEK-293 Cells Using Sodium Butyrate

BioTechniques ◽  
2003 ◽  
Vol 34 (5) ◽  
pp. 968-972 ◽  
Author(s):  
Jürgen Grünberg ◽  
Karin Knogler ◽  
Robert Waibel ◽  
Ilse Novak-Hofer
Keyword(s):  
Sodium Butyrate ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
High Yield ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
Yield Production ◽  
293 Cells ◽  
Recombinant Antibody Fragments ◽  
High Yield Production

Process intensification for high yield production of influenza H1N1 Gag virus-like particles using an inducible HEK-293 stable cell line

Vaccine ◽  
2017 ◽  
Vol 35 (33) ◽  
pp. 4220-4228 ◽  
Author(s):  
Alina Venereo-Sanchez ◽  
Melanie Simoneau ◽  
Stéphane Lanthier ◽  
Parminder Chahal ◽  
Lucie Bourget ◽  
...  
Keyword(s):  
Cell Line ◽  
Process Intensification ◽  
High Yield ◽  
Stable Cell Line ◽  
Hek 293 ◽  
Virus Like Particles ◽  
Yield Production ◽  
High Yield Production ◽  
Influenza H1n1

scholarly journals High-yield expression of periplasmic single-chain variable fragments by solid Escherichia coli cultures

BioTechniques ◽  
2021 ◽  
Author(s):  
Yoshiro Hanyu ◽  
Mieko Kato
Keyword(s):  
Escherichia Coli ◽  
Liquid Culture ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
High Yield ◽  
Single Chain ◽  
E Coli ◽  
Recombinant Antibody Fragments ◽  
Single Chain Variable Fragments ◽  
High Yields

High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in Escherichia coli using liquid cultures; however, their yields and quality are often poor. Here the authors expressed a single-chain variable fragment in E. coli cultivated on the wet surface of a solid support. Compared with a liquid culture, the authors obtained 2.5-times more single-chain variable fragments with membrane-cultivated E. coli. This method has two important advantages: it enables high yields of periplasmic single-chain variable fragments compared with liquid culture and offers simple and rapid expression and extraction.

scholarly journals Review: Managing sheep and goats for sustainable high yield production

animal ◽  
2021 ◽  
pp. 100293
Author(s):  
J. Simões ◽  
J.A. Abecia ◽  
A. Cannas ◽  
J.A. Delgadillo ◽  
D. Lacasta ◽  
...  
Keyword(s):  
High Yield ◽  
Sheep And Goats ◽  
Yield Production ◽  
High Yield Production

scholarly journals High‐Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum “magneticum”

2021 ◽  
pp. 2101017
Author(s):  
Frank Mickoleit ◽  
Sabine Rosenfeldt ◽  
Mauricio Toro‐Nahuelpan ◽  
Miroslava Schaffer ◽  
Anna S. Schenk ◽  
...  
Keyword(s):  
Rhodospirillum Rubrum ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

High yield production of extracellular recombinant levansucrase by Bacillus megaterium

2012 ◽  
Vol 97 (8) ◽  
pp. 3343-3353 ◽  
Author(s):  
Claudia Korneli ◽  
Rebekka Biedendieck ◽  
Florian David ◽  
Dieter Jahn ◽  
Christoph Wittmann
Keyword(s):  
Bacillus Megaterium ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

scholarly journals Characteristics and requirements of basal autophagy in HEK 293 cells

Autophagy ◽  
2013 ◽  
Vol 9 (9) ◽  
pp. 1407-1417 ◽  
Author(s):  
Patience Musiwaro ◽  
Matthew Smith ◽  
Maria Manifava ◽  
Simon A. Walker ◽  
Nicholas T. Ktistakis
Keyword(s):  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells
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