scholarly journals Analysis of Multiple Gene Expression Array Experiments after Repetitive Hybridizations on Nylon Membranes

BioTechniques ◽  
2002 ◽  
Vol 33 (1) ◽  
pp. 108-117 ◽  
Author(s):  
Jorrit J. Hornberg ◽  
Richard R. de Haas ◽  
Henk Dekker ◽  
Jan Lankelma
Keyword(s):  
Gene Expression ◽  
Gene Expression Array ◽  
Expression Array ◽  
Multiple Gene

Sa1165 - A Gene Expression Array as a Diagnostic Test in Pediatric Eosinophilic Esophagitis

2018 ◽  
Vol 154 (6) ◽  
pp. S-265-S-266
Author(s):  
Christopher Foster ◽  
Sydney Chatfield ◽  
Todd Jensen ◽  
Zhu Wang ◽  
Christine Finck ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diagnostic Test ◽  
Eosinophilic Esophagitis ◽  
Gene Expression Array ◽  
Expression Array

scholarly journals Solar simulated ultraviolet radiation inactivates HCoV-NL63 and SARS-CoV-2 coronaviruses at environmentally relevant doses

2021 ◽  
Author(s):  
Georg T. Wondrak ◽  
Jana Jandova ◽  
Spencer J. Williams ◽  
Dominik Schenten
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Uv Light ◽  
Gene Expression Array ◽  
Array Analysis ◽  
Expression Array ◽  
Response Gene ◽  
Full Spectrum ◽  
Solar Uv ◽  
Stress Response Gene

The germicidal properties of short wavelength ultraviolet C (UVC) light are well established and used to inactivate many viruses and other microbes. However, much less is known about germicidal effects of terrestrial solar UV light, confined exclusively to wavelengths in the UVA and UVB regions. Here, we have explored the sensitivity of the human coronaviruses HCoV-NL63 and SARS-CoV-2 to solar-simulated full spectrum ultraviolet light (sUV) delivered at environmentally relevant doses. First, HCoV-NL63 coronavirus inactivation by sUV-exposure was confirmed employing (i) viral plaque assays, (ii) RT-qPCR detection of viral genome replication, and (iii) infection-induced stress response gene expression array analysis. Next, a detailed dose-response relationship of SARS-CoV-2 coronavirus inactivation by sUV was elucidated, suggesting a half maximal suppression of viral infectivity at low sUV doses. Likewise, extended sUV exposure of SARS-CoV-2 blocked cellular infection as revealed by plaque assay and stress response gene expression array analysis. Moreover, comparative (HCoV-NL63 versus SARS-CoV-2) single gene expression analysis by RT-qPCR confirmed that sUV exposure blocks coronavirus-induced redox, inflammatory, and proteotoxic stress responses. Based on our findings, we estimate that solar ground level full spectrum UV light impairs coronavirus infectivity at environmentally relevant doses. Given the urgency and global scale of the unfolding SARS-CoV-2 pandemic, these prototype data suggest feasibility of solar UV-induced viral inactivation, an observation deserving further molecular exploration in more relevant exposure models.

scholarly journals Detection ofCEBPADouble Mutants in Acute Myeloid Leukemia Using a Custom Gene Expression Array

2013 ◽  
Vol 17 (5) ◽  
pp. 395-400 ◽  
Author(s):  
Martin H. van Vliet ◽  
Pia Burgmer ◽  
Linda de Quartel ◽  
Jaap P.L. Brand ◽  
Leonie C.M. de Best ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Gene Expression Array ◽  
Expression Array ◽  
Acute Myeloid

Demonstration of diet-induced decoupling of fatty acid and cholesterol synthesis by combining gene expression array and 2H2O quantification

2012 ◽  
Vol 302 (2) ◽  
pp. E209-E217 ◽  
Author(s):  
Kristian K. Jensen ◽  
Stephen F. Previs ◽  
Lei Zhu ◽  
Kithsiri Herath ◽  
Sheng-Ping Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Expression Analysis ◽  
Cholesterol Synthesis ◽  
Gene Expression Analysis ◽  
Cholesterol Biosynthesis ◽  
Acid Synthesis ◽  
Gene Expression Array ◽  
Expression Array ◽  
High Carbohydrate

The liver is a crossroad for metabolism of lipid and carbohydrates, with acetyl-CoA serving as an important metabolic intermediate and a precursor for fatty acid and cholesterol biosynthesis pathways. A better understanding of the regulation of these pathways requires an experimental approach that provides both quantitative metabolic flux measurements and mechanistic insight. Under conditions of high carbohydrate availability, excess carbon is converted into free fatty acids and triglyceride for storage, but it is not clear how excessive carbohydrate availability affects cholesterol biosynthesis. To address this, C57BL/6J mice were fed either a low-fat, high-carbohydrate diet or a high-fat, carbohydrate-free diet. At the end of the dietary intervention, the two groups received 2H2O to trace de novo fatty acid and cholesterol synthesis, and livers were collected for gene expression analysis. Expression of lipid and glucose metabolism genes was determined using a custom-designed pathway focused PCR-based gene expression array. The expression analysis showed downregulation of cholesterol biosynthesis genes and upregulation of fatty acid synthesis genes in mice receiving the high-carbohydrate diet compared with the carbohydrate-free diet. In support of these findings, 2H2O tracer data showed that fatty acid synthesis was increased 10-fold and cholesterol synthesis was reduced by 1.6-fold in mice fed the respective diets. In conclusion, by applying gene expression analysis and tracer methodology, we show that fatty acid and cholesterol synthesis are differentially regulated when the carbohydrate intake in mice is altered.

Identification of differentially expressed genes in organ-confined prostate cancer by gene expression array

The Prostate ◽  
2001 ◽  
Vol 47 (2) ◽  
pp. 132-140 ◽  
Author(s):  
Albert Chetcuti ◽  
Sienna Margan ◽  
Stephen Mann ◽  
Peter Russell ◽  
David Handelsman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Gene Expression Array ◽  
Expression Array
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