scholarly journals Removal of PolyA Tails from Full-Length cDNA Libraries for High-Efficiency Sequencing

BioTechniques ◽  
2001 ◽  
Vol 31 (5) ◽  
pp. 1042-1049 ◽  
Author(s):  
Y. Shibata ◽  
P. Carninci ◽  
K. Sato ◽  
N. Hayatsu ◽  
T. Shiraki ◽  
...  
Keyword(s):  
High Efficiency ◽  
Full Length ◽  
Cdna Libraries ◽  
Full Length Cdna

scholarly journals High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper

1998 ◽  
Vol 15 (5) ◽  
pp. 707-720 ◽  
Author(s):  
Motoaki Seki ◽  
Piero Carninci ◽  
Yoko Nishiyama ◽  
Yoshihide Hayashizaki ◽  
Kazuo Shinozaki
Keyword(s):  
High Efficiency ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts

BioTechniques ◽  
2003 ◽  
Vol 35 (3) ◽  
pp. 510-518 ◽  
Author(s):  
Tomoko Hirozane-Kishikawa ◽  
Toshiyuki Shiraki ◽  
Kazunori Waki ◽  
Mari Nakamura ◽  
Takahiro Arakawa ◽  
...  
Keyword(s):  
Full Length ◽  
Cdna Libraries ◽  
Full Length Cdna

High-efficiency cloning of full-length cDNA

1982 ◽  
Vol 2 (2) ◽  
pp. 161-170
Author(s):  
H Okayama ◽  
P Berg
Keyword(s):  
Plasmid Dna ◽  
High Efficiency ◽  
Full Length ◽  
Mrna Sequence ◽  
Beta Globin ◽  
Cdna Synthesis ◽  
Globin Mrna ◽  
Full Length Cdna ◽  
Coding Regions ◽  
Entire Nucleotide Sequence

A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per microgram of rabbit reticulocyte mRNA, about 10% contained a complete alpha- of beta-globin mRNA sequence, and at least 30 to 50%, but very likely more, contained the entire globin coding regions. We attribute the high efficiency of cloning full- or nearly full-length cDNA to (i) the fact that the plasmid DNA vector itself serves as the primer for first- and second-strand cDNA synthesis, (ii) the lack of any nuclease treatment of the products, and (iii) the fact that one of the steps in the procedure results in preferential cloning of recombinants with full-length cDNA's over those with truncated cDNA's.

A novel method for development of malaria vaccines using full-length cDNA libraries

Vaccine ◽  
2005 ◽  
Vol 23 (34) ◽  
pp. 4359-4366 ◽  
Author(s):  
Akiko Shibui ◽  
Takashi Shiibashi ◽  
Sadao Nogami ◽  
Sumio Sugano ◽  
Junichi Watanabe
Keyword(s):  
Full Length ◽  
Cdna Libraries ◽  
Full Length Cdna ◽  
Malaria Vaccines ◽  
Novel Method
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