scholarly journals PNA-Based Light-Up Probes for Real-Time Detection of Sequence-Specific PCR Products

BioTechniques ◽  
2001 ◽  
Vol 31 (4) ◽  
pp. 766-771 ◽  
Author(s):  
Petra Wolffs ◽  
Rickard Knutsson ◽  
Robert Sjöback ◽  
Peter Rådström
Keyword(s):  
Real Time ◽  
Specific Pcr ◽  
Pcr Products ◽  
Real Time Detection

Statistical Diagnostics Emerging from External Quality Control of Real-Time PCR

2004 ◽  
Vol 19 (2) ◽  
pp. 141-146 ◽  
Author(s):  
E. Marubini ◽  
P. Verderio ◽  
C. Casini Raggi ◽  
M. Pazzagli ◽  
C. Orlando
Keyword(s):  
Quality Control ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Charts ◽  
Reference Curve ◽  
External Quality Control ◽  
External Quality ◽  
Standard Curve ◽  
Pcr Products ◽  
Real Time Detection

Besides the application of conventional qualitative PCR as a valuable tool to enrich or identify specific sequences of nucleic acids, a new revolutionary technique for quantitative PCR determination has been introduced recently. It is based on real-time detection of PCR products revealed as a homogeneous accumulating signal generated by specific dyes. However, as far as we know, the influence of the variability of this technique on the reliability of the quantitative assay has not been thoroughly investigated. A national program of external quality assurance (EQA) for real-time PCR determination involving 42 Italian laboratories has been developed to assess the analytical performance of real-time PCR procedures. Participants were asked to perform a conventional experiment based on the use of an external reference curve (standard curve) for real-time detection of three cDNA samples with different concentrations of a specific target. In this paper the main analytical features of the standard curve have been investigated in an attempt to produce statistical diagnostics emerging from external quality control. Specific control charts were drawn to help biochemists take technical decisions aimed at improving the performance of their laboratories. Overall, our results indicated a subset of seven laboratories whose performance appeared to be markedly outside the limits for at least one of the standard curve features investigated. Our findings suggest the usefulness of the approach presented here for monitoring the heterogeneity of results produced by different laboratories and for selecting those laboratories that need technical advice on their performance.

Qualitative and event-specific PCR real-time detection methods for StarLink maize

2003 ◽  
Vol 216 (3) ◽  
pp. 259-263 ◽  
Author(s):  
Pieter Windels ◽  
Sophie Bertrand ◽  
Ann Depicker ◽  
William Moens ◽  
Erik Van Bockstaele ◽  
...  
Keyword(s):  
Real Time ◽  
Detection Methods ◽  
Specific Pcr ◽  
Real Time Detection

Real-Time Detection of Drowsiness Related Lane Departures Using Steering Wheel Angle

2012 ◽  
Author(s):  
Anthony D. McDonald ◽  
Chris Schwarz ◽  
John D. Lee ◽  
Timothy L. Brown
Keyword(s):  
Real Time ◽  
Steering Wheel ◽  
Real Time Detection
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